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Effect of sodium butyrate on ABC transporters in lung cancer A549 and colorectal cancer HCT116 cells

  • Authors:
    • Bin Shi
    • Fang‑Fang Xu
    • Cai‑Ping Xiang
    • Ru Jia
    • Chun-Hong Yan
    • Se-Qing Ma
    • Ning Wang
    • An-Jiao Wang
    • Ping Fan
  • View Affiliations / Copyright

    Affiliations: Department of Anorectal Surgery, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230000, P.R. China, Department of Anorectal Surgery, The Second Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, Anhui 230000, P.R. China
    Copyright: © Shi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 148
    |
    Published online on: August 24, 2020
       https://doi.org/10.3892/ol.2020.12011
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Abstract

Histone deacetylase (HDAC) inhibitors and DNA alkylators are effective components of combination chemotherapy. The aim of the present study was to investigate the possible mechanism of their synergism by detecting the effect of HDAC inhibitors on the expression levels of drug transporters that export DNA alkylators. It was demonstrated that the HDAC inhibitor sodium butyrate (NaB) induced the differential expression of multidrug resistant ATP‑binding cassette (ABC) transporters in lung cancer and colorectal cancer cells. Specifically, NaB increased the mRNA expression levels of ABC subfamily B member 1 (ABCB1), ABCC10 and ABCC12, and protein expression levels of multidrug resistance‑1 (MDR1), multidrug resistance‑associated protein 7 (MRP7) and MRP9. Moreover, NaB decreased the expression levels of ABCC1, ABCC2 and ABCC3 mRNAs, as well as those of MRP1, MRP2 and MRP3 proteins. The molecular mechanism underlying this process was subsequently investigated. NaB decreased the expression of HDAC4, but not HDAC1, HDAC2 or HDAC3. In addition, NaB promoted histone H3 acetylation and methylation at lysine 9, as well as MDR1 acetylation, suggesting that acetylation and methylation may be involved in NaB‑mediated ABC transporter expression. Thus, the present results indicated that the synergism of the HDAC inhibitors with the DNA alkylating agents may due to the inhibitory effect of MRPs by HDAC inhibitors. The findings also suggested the possibility of antagonistic effects following the combined treatment of HDAC inhibitors with MDR1 ligands.
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Copy and paste a formatted citation
Spandidos Publications style
Shi B, Xu FF, Xiang CP, Jia R, Yan C, Ma S, Wang N, Wang A and Fan P: Effect of sodium butyrate on ABC transporters in lung cancer A549 and colorectal cancer HCT116 cells. Oncol Lett 20: 148, 2020.
APA
Shi, B., Xu, F., Xiang, C., Jia, R., Yan, C., Ma, S. ... Fan, P. (2020). Effect of sodium butyrate on ABC transporters in lung cancer A549 and colorectal cancer HCT116 cells. Oncology Letters, 20, 148. https://doi.org/10.3892/ol.2020.12011
MLA
Shi, B., Xu, F., Xiang, C., Jia, R., Yan, C., Ma, S., Wang, N., Wang, A., Fan, P."Effect of sodium butyrate on ABC transporters in lung cancer A549 and colorectal cancer HCT116 cells". Oncology Letters 20.5 (2020): 148.
Chicago
Shi, B., Xu, F., Xiang, C., Jia, R., Yan, C., Ma, S., Wang, N., Wang, A., Fan, P."Effect of sodium butyrate on ABC transporters in lung cancer A549 and colorectal cancer HCT116 cells". Oncology Letters 20, no. 5 (2020): 148. https://doi.org/10.3892/ol.2020.12011
Copy and paste a formatted citation
x
Spandidos Publications style
Shi B, Xu FF, Xiang CP, Jia R, Yan C, Ma S, Wang N, Wang A and Fan P: Effect of sodium butyrate on ABC transporters in lung cancer A549 and colorectal cancer HCT116 cells. Oncol Lett 20: 148, 2020.
APA
Shi, B., Xu, F., Xiang, C., Jia, R., Yan, C., Ma, S. ... Fan, P. (2020). Effect of sodium butyrate on ABC transporters in lung cancer A549 and colorectal cancer HCT116 cells. Oncology Letters, 20, 148. https://doi.org/10.3892/ol.2020.12011
MLA
Shi, B., Xu, F., Xiang, C., Jia, R., Yan, C., Ma, S., Wang, N., Wang, A., Fan, P."Effect of sodium butyrate on ABC transporters in lung cancer A549 and colorectal cancer HCT116 cells". Oncology Letters 20.5 (2020): 148.
Chicago
Shi, B., Xu, F., Xiang, C., Jia, R., Yan, C., Ma, S., Wang, N., Wang, A., Fan, P."Effect of sodium butyrate on ABC transporters in lung cancer A549 and colorectal cancer HCT116 cells". Oncology Letters 20, no. 5 (2020): 148. https://doi.org/10.3892/ol.2020.12011
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