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Article Open Access

Silencing glioma‑associated oncogene homolog 1 suppresses the migration and invasion of hepatocellular carcinoma in vitro

  • Authors:
    • Zeming Hu
    • Fangfang Xie
    • Ang Hu
    • Mengjing Xu
    • Yuwen Liu
    • Jiankang  Zhang
    • Jianbo Xiao
    • Yunlei Song
    • Jianing Zhong
    • Bin Chen
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, The First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi 341000, P.R. China, Key Laboratory of Prevention and Treatment of Cardiovascular and Cerebrovascular Diseases, Ministry of Education, Gannan Medical University, Ganzhou, Jiangxi 341000, P.R. China
    Copyright: © Hu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 228
    |
    Published online on: September 11, 2020
       https://doi.org/10.3892/ol.2020.12091
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Abstract

Hepatocellular carcinoma (HCC) is the fourth most common cause of cancer‑associated death worldwide. Glioma‑associated oncogene homolog 1 (Gli1) is a key component and functions as a reliable marker of Hedgehog signaling pathway activation. Previous studies have demonstrated that Gli1 serves important roles in the progression of various types of cancer, including HCC. However, its effect on HCC invasion and metastasis and the underlying mechanism remain to be elucidated. Small interference RNA was employed to silence the Gli1 gene in liver cancer cells. Reverse transcription‑quantitative PCR and western blot analysis were performed to evaluate the mRNA and protein expression of Gli1, respectively. A series of assays, including Cell Counting Kit‑8, adhesion, wound healing and Matrigel invasion were performed to investigate cell viability, adhesive, migratory and invasive capabilities of liver cancer cells, respectively. In addition, immunofluorescence staining was performed to determine the cellular localization of focal adhesion kinase (FAK), phosphorylated (p‑)FAK and p‑AKT. The mRNA and protein expression of Gli1 in liver cancer cells (HepG2 and SK‑Hep1) were markedly decreased in a dose‑dependent manner following Gli1‑knockdown. Gli1 silencing significantly inhibited the adhesion, migration and invasion of SK‑Hep1 cells. Additionally, knockdown of Gli1 markedly suppressed the expression of metalloproteinase (MMP)‑2 and MMP‑9. Furthermore, downregulation of Gli1 blocked the FAK/AKT signaling pathway. Gli1 serves significant roles in the migration and invasion of HCC cells through activation of the FAK/AKT signaling pathway and subsequent upregulation of MMP‑2 and MMP‑9 expression. Thus, Gli1 may be a potential protein target for the regulation of HCC migration and invasion.
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Copy and paste a formatted citation
Spandidos Publications style
Hu Z, Xie F, Hu A, Xu M, Liu Y, Zhang J, Xiao J, Song Y, Zhong J, Chen B, Chen B, et al: Silencing glioma‑associated oncogene homolog 1 suppresses the migration and invasion of hepatocellular carcinoma <em>in&nbsp;vitro</em>. Oncol Lett 20: 228, 2020.
APA
Hu, Z., Xie, F., Hu, A., Xu, M., Liu, Y., Zhang, J. ... Chen, B. (2020). Silencing glioma‑associated oncogene homolog 1 suppresses the migration and invasion of hepatocellular carcinoma <em>in&nbsp;vitro</em>. Oncology Letters, 20, 228. https://doi.org/10.3892/ol.2020.12091
MLA
Hu, Z., Xie, F., Hu, A., Xu, M., Liu, Y., Zhang, J., Xiao, J., Song, Y., Zhong, J., Chen, B."Silencing glioma‑associated oncogene homolog 1 suppresses the migration and invasion of hepatocellular carcinoma <em>in&nbsp;vitro</em>". Oncology Letters 20.5 (2020): 228.
Chicago
Hu, Z., Xie, F., Hu, A., Xu, M., Liu, Y., Zhang, J., Xiao, J., Song, Y., Zhong, J., Chen, B."Silencing glioma‑associated oncogene homolog 1 suppresses the migration and invasion of hepatocellular carcinoma <em>in&nbsp;vitro</em>". Oncology Letters 20, no. 5 (2020): 228. https://doi.org/10.3892/ol.2020.12091
Copy and paste a formatted citation
x
Spandidos Publications style
Hu Z, Xie F, Hu A, Xu M, Liu Y, Zhang J, Xiao J, Song Y, Zhong J, Chen B, Chen B, et al: Silencing glioma‑associated oncogene homolog 1 suppresses the migration and invasion of hepatocellular carcinoma <em>in&nbsp;vitro</em>. Oncol Lett 20: 228, 2020.
APA
Hu, Z., Xie, F., Hu, A., Xu, M., Liu, Y., Zhang, J. ... Chen, B. (2020). Silencing glioma‑associated oncogene homolog 1 suppresses the migration and invasion of hepatocellular carcinoma <em>in&nbsp;vitro</em>. Oncology Letters, 20, 228. https://doi.org/10.3892/ol.2020.12091
MLA
Hu, Z., Xie, F., Hu, A., Xu, M., Liu, Y., Zhang, J., Xiao, J., Song, Y., Zhong, J., Chen, B."Silencing glioma‑associated oncogene homolog 1 suppresses the migration and invasion of hepatocellular carcinoma <em>in&nbsp;vitro</em>". Oncology Letters 20.5 (2020): 228.
Chicago
Hu, Z., Xie, F., Hu, A., Xu, M., Liu, Y., Zhang, J., Xiao, J., Song, Y., Zhong, J., Chen, B."Silencing glioma‑associated oncogene homolog 1 suppresses the migration and invasion of hepatocellular carcinoma <em>in&nbsp;vitro</em>". Oncology Letters 20, no. 5 (2020): 228. https://doi.org/10.3892/ol.2020.12091
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