TUFM‑knockdown inhibits the migration and proliferation of gastrointestinal stromal tumor cells

Weng,Xiaoyuan; Zheng,Song; Shui,Hanli; Lin,Guosheng; Zhou,Yongjian

doi:10.3892/ol.2020.12113

TUFM‑knockdown inhibits the migration and proliferation of gastrointestinal stromal tumor cells

Authors:
- Xiaoyuan Weng
- Song Zheng
- Hanli Shui
- Guosheng Lin
- Yongjian Zhou
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Affiliations: Department of Surgery, Department of Clinical Medicine, Quanzhou Medical College, Quanzhou, Fujian 362010, P.R. China, Department of Medical Oncology, Affiliated Hangzhou First People's Hospital, Zhejiang Chinese Medical University Affiliated Hangzhou First Hospital, Hangzhou, Zhejiang 310006, P.R. China, Department of Gastric Surgery, Union Hospital of Fujian Medical University, Fuzhou, Fujian 350001, P.R. China
Published online on: September 17, 2020 https://doi.org/10.3892/ol.2020.12113
Article Number: 250
Copyright: © Weng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Gastrointestinal stromal tumors (GISTs) are the most common pathologic type of mesenchymal tumor in the digestive tract. Patients with GIST face the risk of metastasis, postoperative recurrence and imatinib mesylate (IM) resistance. Mitochondrial Tu translation elongation factor (TUFM) is highly expressed in GISTs, and is associated with oncogenesis, progression and prognosis. There is evidence that TUFM is involved in tumor invasion and metastasis. However, the effect of TUFM on GIST‑T1 cells and the IM‑resistant GIST‑IR cell line remains unclear. The present study aimed to evaluate the effects of TUFM on the proliferation, migration and apoptosis of GIST cells in vitro. TUFM short hairpin (sh)RNA expression plasmids were transfected into GIST‑T1 and GIST‑IR cells by electroporation. The expression levels of enhanced green fluorescent protein were observed by fluorescence microscopy to evaluate the electroporation efficiency. The expression levels of TUFM were detected by western blot analysis and reverse transcription‑quantitative PCR. Cell proliferation was assessed by counting cells and using a Cell Counting Kit‑8 assay. Cell migration was analyzed using wound healing and Transwell migration assays. Cell cycle distribution and late apoptosis were assessed by flow cytometry. TUFM shRNA expression plasmids were successfully transfected into the GIST cell line by electroporation. The transfection efficiency was >75%, and the TUFM gene silencing efficiency was 73.2±1.4%. TUFM‑knockdown decreased the proliferation and migration capacity of GIST‑T1 and GIST‑IR cells. The proportion of cells in the pre‑G1 stage was increased without change in the proportions of cells in the G1, S and G2/M stages after TUFM silencing in GIST‑T1 and GIST‑IR cells. TUFM may be related to GIST infiltration and metastatic recurrence, suggesting that TUFM may be an effective target for preventing the progression and metastasis of GISTs.

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