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Serpin peptidase inhibitor clade A member 1‑overexpression in gastric cancer promotes tumor progression in vitro and is associated with poor prognosis

  • Authors:
    • Longchang Jiang
    • Liangbiao George Hu
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    Affiliations: Translational Safety and Bioanalytical Sciences, Amgen Research, Amgen Asia Research and Development Center, Shanghai 201210, P.R. China
    Copyright: © Jiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 278
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    Published online on: September 23, 2020
       https://doi.org/10.3892/ol.2020.12141
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Abstract

Gastric cancer is the second most common cause of cancer‑associated death in Asia. The incidence and mortality rates of gastric cancer have markedly increased in the past few decades. Therefore, the identification of novel gastric cancer biomarkers are needed to determine prognosis. The role of serpin peptidase inhibitor clade A member 1 (SERPINA1) has been studied in several types of cancer; however, little is known about its mechanism in gastric cancer. The present study aimed to evaluate SERPINA1 as a potential prognostic biomarker in gastric cancer and to identify the possible mechanisms underlying its action. The expression levels of SERPINA1 in several gastric cancer datasets were assessed, and it was identified that high expression of SERPINA1 was associated to poor clinical outcomes. Furthermore, using histochemical analysis, western blotting, apoptotic analysis, gap closure and invasion assays in cell lines, it was reported that silencing of SERPINA1 inhibited the formation of cellular pseudopodia and did not affect apoptosis, but promoted cell cycle S‑phase entry. In addition, overexpression of SERPINA1 increased the migration and invasion of gastric cancer cells, whereas knockdown of SERPINA1 decreased these functions. Moreover, SERPINA1 overexpression increased the protein levels of SMAD4, which is a key regulator of the transforming growth factor (TGF)‑β signaling pathway. Taken together, the present data demonstrated that SERPINA1 promotes gastric cancer progression through TGF‑β signaling, and suggested that SERPINA1 may be a novel prognostic biomarker from tumor tissue biopsy in gastric cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Jiang L and Hu LG: Serpin peptidase inhibitor clade&nbsp;A member&nbsp;1‑overexpression in gastric cancer promotes tumor progression <em>in&nbsp;vitro</em> and is associated with poor prognosis. Oncol Lett 20: 278, 2020.
APA
Jiang, L., & Hu, L.G. (2020). Serpin peptidase inhibitor clade&nbsp;A member&nbsp;1‑overexpression in gastric cancer promotes tumor progression <em>in&nbsp;vitro</em> and is associated with poor prognosis. Oncology Letters, 20, 278. https://doi.org/10.3892/ol.2020.12141
MLA
Jiang, L., Hu, L. G."Serpin peptidase inhibitor clade&nbsp;A member&nbsp;1‑overexpression in gastric cancer promotes tumor progression <em>in&nbsp;vitro</em> and is associated with poor prognosis". Oncology Letters 20.6 (2020): 278.
Chicago
Jiang, L., Hu, L. G."Serpin peptidase inhibitor clade&nbsp;A member&nbsp;1‑overexpression in gastric cancer promotes tumor progression <em>in&nbsp;vitro</em> and is associated with poor prognosis". Oncology Letters 20, no. 6 (2020): 278. https://doi.org/10.3892/ol.2020.12141
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang L and Hu LG: Serpin peptidase inhibitor clade&nbsp;A member&nbsp;1‑overexpression in gastric cancer promotes tumor progression <em>in&nbsp;vitro</em> and is associated with poor prognosis. Oncol Lett 20: 278, 2020.
APA
Jiang, L., & Hu, L.G. (2020). Serpin peptidase inhibitor clade&nbsp;A member&nbsp;1‑overexpression in gastric cancer promotes tumor progression <em>in&nbsp;vitro</em> and is associated with poor prognosis. Oncology Letters, 20, 278. https://doi.org/10.3892/ol.2020.12141
MLA
Jiang, L., Hu, L. G."Serpin peptidase inhibitor clade&nbsp;A member&nbsp;1‑overexpression in gastric cancer promotes tumor progression <em>in&nbsp;vitro</em> and is associated with poor prognosis". Oncology Letters 20.6 (2020): 278.
Chicago
Jiang, L., Hu, L. G."Serpin peptidase inhibitor clade&nbsp;A member&nbsp;1‑overexpression in gastric cancer promotes tumor progression <em>in&nbsp;vitro</em> and is associated with poor prognosis". Oncology Letters 20, no. 6 (2020): 278. https://doi.org/10.3892/ol.2020.12141
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