Open Access

miR‑1301‑3p promotes the proliferation and migration of lung cancer cells via direct repression of polymerase I and transcript release factor

  • Authors:
    • Yun Wu
    • Qianwen Shen
    • Xiaoyu Chen
    • Yue Wu
    • Yuxu Niu
    • Fanzhen Lv
  • View Affiliations

  • Published online on: September 23, 2020     https://doi.org/10.3892/ol.2020.12149
  • Article Number: 286
  • Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Aberrant expression of microRNAs (miRNAs or miRs) is associated with a number of human diseases, including lung cancer. Although numerous differentially expressed miRNAs have been identified in lung cancer via microarray and sequencing methods, to the best of our knowledge, only a small portion of these miRNAs have been experimentally verified. In the present study, miR‑1301‑3p expression levels in lung tumor tissues and lung cancer cells were measured by reverse transcription‑quantitative PCR (RT‑qPCR) and by analyzing previously published data. Cell Counting Kit‑8 and Transwell assays were used to analyze the function of miR‑1301‑3p in lung cancer tissues and cells. Bioinformatics analysis, RT‑qPCR, western blotting and a dual‑luciferase reporter assay were performed to investigate the mechanism of miR‑1301‑3p in lung cancer cells. It was identified that miR‑1301‑3p is an upregulated miRNA in lung cancer via analyzing previously published microarray and The Cancer Genome Atlas‑lung squamous cell carcinoma project data, and the upregulation of miR‑1301‑3p was confirmed in collected clinical samples and cells. Inhibition of miR‑1301‑3p suppressed lung cancer cell proliferation and migration. In addition, miR‑1301‑3p inhibition upregulated E‑cadherin, an epithelial cell maker, and downregulated vimentin, a mesenchymal cell marker. Using bioinformatics analysis, it was revealed that polymerase I and transcript release factor (PTRF) is a target of miR‑1301‑3p. RT‑qPCR, western blotting and dual‑luciferase reporter assays demonstrated that PTRF is targeted by miR‑1301‑3p in lung cancer cells. The rescue experiments indicated that silencing PTRF could attenuate the inhibition of cell proliferation and migration induced by miR‑1301‑3p inhibitor in lung cancer cells. Furthermore, a strong negative correlation between miR‑1301‑3p and PTRF mRNA was identified in clinical samples. In summary, the present data highlight the involvement of miR‑1301‑3p in the proliferation and migration of lung cancer cells, indicating that miR‑1301‑3p may be a promising biomarker for lung cancer.
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December-2020
Volume 20 Issue 6

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Copy and paste a formatted citation
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Spandidos Publications style
Wu Y, Shen Q, Chen X, Wu Y, Niu Y and Lv F: miR‑1301‑3p promotes the proliferation and migration of lung cancer cells via direct repression of polymerase I and transcript release factor. Oncol Lett 20: 286, 2020
APA
Wu, Y., Shen, Q., Chen, X., Wu, Y., Niu, Y., & Lv, F. (2020). miR‑1301‑3p promotes the proliferation and migration of lung cancer cells via direct repression of polymerase I and transcript release factor. Oncology Letters, 20, 286. https://doi.org/10.3892/ol.2020.12149
MLA
Wu, Y., Shen, Q., Chen, X., Wu, Y., Niu, Y., Lv, F."miR‑1301‑3p promotes the proliferation and migration of lung cancer cells via direct repression of polymerase I and transcript release factor". Oncology Letters 20.6 (2020): 286.
Chicago
Wu, Y., Shen, Q., Chen, X., Wu, Y., Niu, Y., Lv, F."miR‑1301‑3p promotes the proliferation and migration of lung cancer cells via direct repression of polymerase I and transcript release factor". Oncology Letters 20, no. 6 (2020): 286. https://doi.org/10.3892/ol.2020.12149