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Linalool inhibits 22Rv1 prostate cancer cell proliferation and induces apoptosis

  • Authors:
    • Yunqi Zhao
    • Xianliang Cheng
    • Guohui Wang
    • Yuan Liao
    • Chen Qing
  • View Affiliations / Copyright

    Affiliations: School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming, Yunnan 650500, P.R. China
    Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 289
    |
    Published online on: September 24, 2020
       https://doi.org/10.3892/ol.2020.12152
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Abstract

Linalool is an unsaturated terpene that can be found in several plants and exhibits various biological activities. The aim of the present study was to investigate the anticancer activity of linalool using the human prostate cancer 22Rv1 cell line. Flow cytometry was employed to study the effects of linalool on the induction of apoptosis, cell cycle progression, loss of mitochondrial membrane potential and cytochrome c release, whereas the effects of linalool on apoptosis‑associated proteins were investigated by western blot analysis. An efficacy study was conducted using 22Rv1 tumor‑bearing mice. The expression of the cell proliferation markers Ki‑67 and proliferating cell nuclear antigen (PCNA) in xenograft tumors was evaluated by immunohistochemistry. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay was used to study the induction of apoptosis in an in vivo model. Linalool exerted an inhibitory effect on 22Rv1 cell proliferation and induced apoptosis in both in vitro and in vivo models. Western blot analysis indicated that both the mitochondria‑mediated intrinsic and death‑receptor‑mediated extrinsic pathways were involved in the induction of apoptosis. Furthermore, linalool significantly reduced the expression of Ki‑67 and PCNA in the 22Rv1 xenograft model. The findings of the present study provide evidence supporting the anti‑proliferative effects of linalool on 22Rv1 human prostate cancer cells, and suggest that linalool may be an effective agent for prostate cancer treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao Y, Cheng X, Wang G, Liao Y and Qing C: Linalool inhibits 22Rv1 prostate cancer cell proliferation and induces apoptosis. Oncol Lett 20: 289, 2020.
APA
Zhao, Y., Cheng, X., Wang, G., Liao, Y., & Qing, C. (2020). Linalool inhibits 22Rv1 prostate cancer cell proliferation and induces apoptosis. Oncology Letters, 20, 289. https://doi.org/10.3892/ol.2020.12152
MLA
Zhao, Y., Cheng, X., Wang, G., Liao, Y., Qing, C."Linalool inhibits 22Rv1 prostate cancer cell proliferation and induces apoptosis". Oncology Letters 20.6 (2020): 289.
Chicago
Zhao, Y., Cheng, X., Wang, G., Liao, Y., Qing, C."Linalool inhibits 22Rv1 prostate cancer cell proliferation and induces apoptosis". Oncology Letters 20, no. 6 (2020): 289. https://doi.org/10.3892/ol.2020.12152
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao Y, Cheng X, Wang G, Liao Y and Qing C: Linalool inhibits 22Rv1 prostate cancer cell proliferation and induces apoptosis. Oncol Lett 20: 289, 2020.
APA
Zhao, Y., Cheng, X., Wang, G., Liao, Y., & Qing, C. (2020). Linalool inhibits 22Rv1 prostate cancer cell proliferation and induces apoptosis. Oncology Letters, 20, 289. https://doi.org/10.3892/ol.2020.12152
MLA
Zhao, Y., Cheng, X., Wang, G., Liao, Y., Qing, C."Linalool inhibits 22Rv1 prostate cancer cell proliferation and induces apoptosis". Oncology Letters 20.6 (2020): 289.
Chicago
Zhao, Y., Cheng, X., Wang, G., Liao, Y., Qing, C."Linalool inhibits 22Rv1 prostate cancer cell proliferation and induces apoptosis". Oncology Letters 20, no. 6 (2020): 289. https://doi.org/10.3892/ol.2020.12152
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