Endothelial transdifferentiation of human HGC‑27 gastric cancer cells <em>in&nbsp;vitro</em>

Chen,Changxin; Hunag,Zhixin; Wang,Mucheng; Huang,Zicheng; Chen,Xiangbo; Huang,Anye; Zheng,Binbin; Wu,Lishan; Liu,Yi; Wang,Xinwen; Xu,Weifeng

doi:10.3892/ol.2020.12166

Endothelial transdifferentiation of human HGC‑27 gastric cancer cells in vitro

Authors:
- Changxin Chen
- Zhixin Hunag
- Mucheng Wang
- Zicheng Huang
- Xiangbo Chen
- Anye Huang
- Binbin Zheng
- Lishan Wu
- Yi Liu
- Xinwen Wang
- Weifeng Xu
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Affiliations: Department of Gastroenterology, Quanzhou First Hospital Affiliated to Fujian Medical University, Quanzhou, Fujian 362000, P.R. China, Department of Thoracic Surgery, Fujian Medical University Union Hospital, Fuzhou, Fujian 350005, P.R. China, Department of Orthopaedics, The Third Affiliated Hospital of Southern Medical University, Guangzhou, Guangdong 510630, P.R. China, Department of Medical Oncology, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, Henan 450008, P.R. China
Published online on: September 29, 2020 https://doi.org/10.3892/ol.2020.12166
Article Number: 303
Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Malignant tumor cells are able to transdifferentiate into other cell types in various tissues or organs. Recent studies have demonstrated the ability of cancer cells to transdifferentiate into functional endothelial cells (ECs). However, whether human gastric cancer (GC) cells are able to transdifferentiate into other cell types has remained largely elusive. Furthermore, whether HGC‑27 cells are able to participate in GC angiogenesis remains to be clarified. In the present study, the HGC‑27 cell line grown under hypoxic conditions for 4 days exhibited the typical ‘flagstone’ appearance, which is typical for cultured ECs. HGC‑27 cells cultured on Matrigel under hypoxic conditions gradually formed net‑like structures. Furthermore, the cultured HGC‑27 cells expressed CD31, CD34 and von Willebrand factor, the molecular markers for ECs, under hypoxic conditions. These results indicated that HGC‑27 cells, cultured under hypoxic conditions, are able to transdifferentiate into EC‑like cells in vitro.

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