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Article

ROS1 rearrangements are uncommon in biliary tract cancers

  • Authors:
    • Francesca Mazzoni
    • Paolo Petreni
    • Enrico Vasile
    • Michele Panebianco
    • Andrea Casadei‑Gardini
    • Francesca Negri
    • Alice Lunghi
    • Serena Pillozzi
    • Caterina Vivaldi
    • Erika Gervasi
    • Giovanni Luca Frassineti
    • Luca Messerini
    • Genny Jocollé
    • Alessandra Bisagni
    • Lorenzo Antonuzzo
    • Giulio Rossi
  • View Affiliations / Copyright

    Affiliations: Medical Oncology Unit, AOU Careggi Hospital, I‑50134 Florence, Italy, Medical Oncology Unit, Alta Val d'Elsa Hospital, I‑53036 Poggibonsi, Italy, Medical Oncology Unit, AOU Pisana Hospital, I‑56126 Pisa, Italy, AUSL‑IRCSS, I‑42122 Reggio Emilia, Italy, Department of Medical Oncology, IRST IRCCS, I‑47014 Meldola, Italy, Medical Oncology Unit, AOU Parma Hospital, I‑43126 Parma, Italy, Medical Oncology Unit, San Luca Hospital, I‑55100 Lucca, Italy, Anatomical Pathology Unit, AOU Careggi Hospital, I‑50134 Florence, Italy, Regional Parini Hospital, I‑11100 Aosta, Italy, Pathology Unit, ASL‑IRCCS, I‑42122 Reggio Emilia, Italy, Operative Unit of Pathologic Anatomy, Santa Maria delle Croci Hospital, I‑48121 Ravenna, Italy
  • Article Number: 316
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    Published online on: October 1, 2020
       https://doi.org/10.3892/ol.2020.12179
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Abstract

Biliary tract cancers (BTCs) are a pool of diseases with poor prognosis and there is no orphan drug available. Currently, no molecular targets have been tested as druggable oncogenic drivers. C‑ros oncogene 1 (ROS1) rearrangements have been previously described in various tumors, including BTCs; however, data regarding their incidence and biological significance are controversial. Therefore, a retrospective multicenter study was performed to assess the incidence of ROS1 rearrangements in BTCs by means of immunohistochemistry and fluorescence in situ hybridization (FISH). The present study failed to demonstrate ROS1 expression in a multicenter series of 150 cases with BTCs and revealed that D4D6 was the most specific clone compared with other ROS1 primary antibodies, namely PA1‑30318 and EPMGHR2. Notably, negative results obtained with D4D6 completely matched to data sorted out by FISH analysis, thus confirming a lack of ROS1 gene rearrangements in BTCs and false positive results when PA1‑30318 and EPMGHR2 clones were used. These results suggest that ROS1 rearrangements may not be targets for molecular therapy of BTCs with specific inhibitors.
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Copy and paste a formatted citation
Spandidos Publications style
Mazzoni F, Petreni P, Vasile E, Panebianco M, Casadei‑Gardini A, Negri F, Lunghi A, Pillozzi S, Vivaldi C, Gervasi E, Gervasi E, et al: <em>ROS1</em> rearrangements are uncommon in biliary tract cancers. Oncol Lett 20: 316, 2020.
APA
Mazzoni, F., Petreni, P., Vasile, E., Panebianco, M., Casadei‑Gardini, A., Negri, F. ... Rossi, G. (2020). <em>ROS1</em> rearrangements are uncommon in biliary tract cancers. Oncology Letters, 20, 316. https://doi.org/10.3892/ol.2020.12179
MLA
Mazzoni, F., Petreni, P., Vasile, E., Panebianco, M., Casadei‑Gardini, A., Negri, F., Lunghi, A., Pillozzi, S., Vivaldi, C., Gervasi, E., Frassineti, G. L., Messerini, L., Jocollé, G., Bisagni, A., Antonuzzo, L., Rossi, G."<em>ROS1</em> rearrangements are uncommon in biliary tract cancers". Oncology Letters 20.6 (2020): 316.
Chicago
Mazzoni, F., Petreni, P., Vasile, E., Panebianco, M., Casadei‑Gardini, A., Negri, F., Lunghi, A., Pillozzi, S., Vivaldi, C., Gervasi, E., Frassineti, G. L., Messerini, L., Jocollé, G., Bisagni, A., Antonuzzo, L., Rossi, G."<em>ROS1</em> rearrangements are uncommon in biliary tract cancers". Oncology Letters 20, no. 6 (2020): 316. https://doi.org/10.3892/ol.2020.12179
Copy and paste a formatted citation
x
Spandidos Publications style
Mazzoni F, Petreni P, Vasile E, Panebianco M, Casadei‑Gardini A, Negri F, Lunghi A, Pillozzi S, Vivaldi C, Gervasi E, Gervasi E, et al: <em>ROS1</em> rearrangements are uncommon in biliary tract cancers. Oncol Lett 20: 316, 2020.
APA
Mazzoni, F., Petreni, P., Vasile, E., Panebianco, M., Casadei‑Gardini, A., Negri, F. ... Rossi, G. (2020). <em>ROS1</em> rearrangements are uncommon in biliary tract cancers. Oncology Letters, 20, 316. https://doi.org/10.3892/ol.2020.12179
MLA
Mazzoni, F., Petreni, P., Vasile, E., Panebianco, M., Casadei‑Gardini, A., Negri, F., Lunghi, A., Pillozzi, S., Vivaldi, C., Gervasi, E., Frassineti, G. L., Messerini, L., Jocollé, G., Bisagni, A., Antonuzzo, L., Rossi, G."<em>ROS1</em> rearrangements are uncommon in biliary tract cancers". Oncology Letters 20.6 (2020): 316.
Chicago
Mazzoni, F., Petreni, P., Vasile, E., Panebianco, M., Casadei‑Gardini, A., Negri, F., Lunghi, A., Pillozzi, S., Vivaldi, C., Gervasi, E., Frassineti, G. L., Messerini, L., Jocollé, G., Bisagni, A., Antonuzzo, L., Rossi, G."<em>ROS1</em> rearrangements are uncommon in biliary tract cancers". Oncology Letters 20, no. 6 (2020): 316. https://doi.org/10.3892/ol.2020.12179
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