Long non‑coding RNA SNHG6 promotes tumorigenesis in melanoma cells via the microRNA‑101‑3p/RAP2B axis Retraction in /10.3892/ol.2024.14274

Zhou,Hong; Li,Lingqiao; Wang,Yingqian; Wang,Dewei

doi:10.3892/ol.2020.12186

Long non‑coding RNA SNHG6 promotes tumorigenesis in melanoma cells via the microRNA‑101‑3p/RAP2B axis

Retraction in: /10.3892/ol.2024.14274

Authors:
- Hong Zhou
- Lingqiao Li
- Yingqian Wang
- Dewei Wang
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Affiliations: Department of Plastic Surgery and Burn, The First People's Hospital of Changzhou, Changzhou, Jiangsu 213000, P.R. China
Published online on: October 5, 2020 https://doi.org/10.3892/ol.2020.12186
Article Number: 323
Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Numerous studies have reported that the long non‑coding RNA (lncRNA) small nucleolar RNA host gene 6 (SNHG6; ENSG00000245910) participates in the development of malignant tumors. However, the underlying mechanism of SNHG6 in the development of melanoma remains unknown. Thus, the present study aimed to investigate the biological role of SNHG6 in the progression of melanoma. SNHG6 expression in melanoma tissues and cells was assessed using a bioinformatics approach and reverse transcription‑quantitative PCR analysis. Cell viability was determined using the Cell Counting Kit‑8 and colony formation assays. The correlation between microRNA (miR)‑101‑3p, SNHG6 and RAP2B expression levels was assessed using Pearson's correlation analysis. Bioinformatic analysis and luciferase reporter assay were utilized to confirm the interaction between miR‑101‑3p and SNHG6 or RAP2B. The Transwell assay was conducted to examine the migratory and invasive activities of melanoma cells. In the present study, SNHG6 expression was upregulated in melanoma tissues and cell lines, and SNHG6 silencing suppressed melanoma cell viability, migration and invasion. SNHG6 was directly bound to miR‑101‑3p, which interacted with RAP2B. In addition, miR‑101‑3p expression was negatively correlated with SNHG6 or RAP2B expression. miR‑101‑3p silencing partially abrogated the suppressive effect of SNHG6‑knockdown on RAP2B expression. Moreover, the data demonstrated that RAP2B overexpression reversed the inhibitory effects on melanoma cell proliferation, migration and invasion induced by SNHG6 silencing. In conclusion, the present study identified that SNHG6 accelerated melanoma progression via regulating the miR‑101‑3p/RAP2B axis. Thus, the SNHG6/miR‑101‑3p/RAP2B signaling pathway may be a novel therapeutic target for melanoma.

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1	Kozar I, Margue C, Rothengatter S, Haan C and Kreis S: Many ways to resistance: How melanoma cells evade targeted therapies. Biochim Biophys Acta Rev Cancer. 1871:313–322. 2019. View Article : Google Scholar : PubMed/NCBI
2	Siegel RL, Miller KD and Jemal A: Cancer statistics, 2017. CA Cancer J Clin. 67:7–30. 2017. View Article : Google Scholar : PubMed/NCBI
3	Clarke CA, McKinley M, Hurley S, Haile RW, Glaser SL, Keegan THM and Swetter SM: Continued increase in melanoma incidence across all socioeconomic status groups in California, 1998–2012. J Invest Dermatol. 137:2282–2290. 2017. View Article : Google Scholar : PubMed/NCBI
4	Slipicevic A and Herlyn M: Narrowing the knowledge gaps for melanoma. Ups J Med Sci. 117:237–243. 2012. View Article : Google Scholar : PubMed/NCBI
5	Gershenwald JE, Scolyer RA, Hess KR, Sondak VK, Long GV, Ross MI, Lazar AJ, Faries MB, Kirkwood JM, McArthur GA, et al: Melanoma staging: Evidence-based changes in the American Joint Committee on Cancer eighth edition cancer staging manual. CA Cancer J Clin. 67:472–492. 2017. View Article : Google Scholar : PubMed/NCBI
6	Baharara J, Amini E, Nikdel N and Salek-Abdollahi F: The cytotoxicity of dacarbazine potentiated by sea cucumber saponin in resistant b16f10 melanoma cells through apoptosis induction. Avicenna J Med Biotechnol. 8:112–119. 2016.PubMed/NCBI
7	Qi P and Du X: The long non-coding RNAs, a new cancer diagnostic and therapeutic gold mine. Mod Pathol. 26:155–165. 2013. View Article : Google Scholar : PubMed/NCBI
8	Luan W, Ding Y, Yuan H, Ma S, Ruan H, Wang J, Lu F and Bu X: Long non-coding RNA LINC00520 promotes the proliferation and metastasis of malignant melanoma by inducing the miR-125b-5p/EIF5A2 axis. J Exp Clin Cancer Res. 39:962020. View Article : Google Scholar : PubMed/NCBI
9	Wan N, Yang W, Cheng H and Wang J: FOXD3-AS1 contributes to the progression of melanoma Via miR-127-3p/FJX1 axis. Cancer Biother Radiopharm. 2020.(Epub ahead of print). View Article : Google Scholar : PubMed/NCBI
10	Yao X, Lan Z, Lai Q, Li A, Liu S and Wang X: lncRNA SNHG6 plays an oncogenic role in colorectal cancer and can be used as a prognostic biomarker for solid tumors. J Cell Physiol. 235:7620–7634. 2020. View Article : Google Scholar : PubMed/NCBI
11	Wang H, Wang L, Tang L, Luo J, Ji H, Zhang W, Zhou J, Li Q and Miao L: Long noncoding RNA SNHG6 promotes proliferation and angiogenesis of cholangiocarcinoma cells through sponging miR-101-3p and activation of E2F8. J Cancer. 11:3002–3012. 2020. View Article : Google Scholar : PubMed/NCBI
12	Li K, Ma YB, Tian YH, Xu XL, Gao Y, He YQ, Pan WT, Zhang JW, He CJ and Wei L: Silencing lncRNA SNHG6 suppresses proliferation and invasion of breast cancer cells through miR-26a/VASP axis. Pathol Res Pract. 215:1525752019. View Article : Google Scholar : PubMed/NCBI
13	Xiao F, Li Y, Wan Y and Xue M: MircroRNA-139 sensitizes ovarian cancer cell to cisplatin-based chemotherapy through regulation of ATP7A/B. Cancer Chemother Pharmacol. 81:935–947. 2018. View Article : Google Scholar : PubMed/NCBI
14	Wei H, He WR, Chen KM, Wang XW and Yi CJ: miR-101 affects proliferation and apoptosis of cervical cancer cells by inhibition of JAK2. Eur Rev Med Pharmacol Sci. 23:5640–5647. 2019.PubMed/NCBI
15	Li L, Shao MY, Zou SC, Xiao ZF and Chen ZC: miR-101-3p inhibits EMT to attenuate proliferation and metastasis in glioblastoma by targeting TRIM44. J Neurooncol. 141:19–30. 2019. View Article : Google Scholar : PubMed/NCBI
16	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
17	Marts AR, Kaine JC, Baum RR, Clayton VL, Bennett JR, Cordonnier LJ, McCarrick R, Hasheminasab A, Crandall LA, Ziegler CJ and Tierney DL: Paramagnetic resonance of cobalt(II) trispyrazolylmethanes and counterion association. Inorg Chem. 56:618–626. 2017. View Article : Google Scholar : PubMed/NCBI
18	Hu Y, Sun H, Hu J and Zhang X: lncRNA DLX6-AS1 promotes the progression of neuroblastoma by activating STAT2 via targeting miR-506-3p. Cancer Manag Res. 12:7451–7463. 2020. View Article : Google Scholar : PubMed/NCBI
19	Qu Y and Liu J: lncRNA MAFG-AS1 contributes to esophageal squamous-cell carcinoma progression via regulating miR143/LASP1. Onco Targets Ther. 13:8359–8370. 2020. View Article : Google Scholar : PubMed/NCBI
20	Ouyang Q, Cui Y, Yang S, Wei W, Zhang M, Zeng J and Qu F: lncRNA MT1JP suppresses biological activities of breast cancer cells in vitro and in vivo by Regulating the miRNA-214/RUNX3 axis. Onco Targets Ther. 13:5033–5046. 2020. View Article : Google Scholar : PubMed/NCBI
21	An LF, Huang JW, Han X and Wang J: Downregulation of lncRNA H19 sensitizes melanoma cells to cisplatin by regulating the miR-18b/IGF1 axis. Anticancer Drugs. 31:473–482. 2020. View Article : Google Scholar : PubMed/NCBI
22	Liu F, Hu L, Pei Y, Zheng K, Wang W, Li S, Qiu E, Shang G, Zhang J and Zhang X: Long non-coding RNA AFAP1-AS1 accelerates the progression of melanoma by targeting miR-653-5p/RAI14 axis. BMC Cancer. 20:2582020. View Article : Google Scholar : PubMed/NCBI
23	Xu JH, Zhao WY, Fang QQ, Wang XF, Zhang DD, Hu YY, Zheng B and Tan WQ: Long noncoding RNA LUADT1 is upregulated in melanoma and may sponge miR-28-5p to upregulate RAP1B. Cancer Biother Radiopharm. 35:307–312. 2020. View Article : Google Scholar : PubMed/NCBI
24	Zou JX and Ge TW: Long non-coding RNA NEAT1 promotes tumor development and metastasis through targeting miR-224-5p in malignant melanoma. Eur Rev Med Pharmacol Sci. 24:1302–1308. 2020.PubMed/NCBI
25	Wu S, Chen H, Zuo L, Jiang H and Yan H: Suppression of Long non-coding RNA MALAT1 inhibits the development of uveal melanoma via microRNA-608-mediated inhibition of HOXC4. Am J Physiol Cell Physiol. 318:C903–C912. 2020. View Article : Google Scholar : PubMed/NCBI
26	Dong Z, Liu H and Zhao G: Long noncoding RNA SNHG6 promotes proliferation and inhibits apoptosis in non-small cell lung cancer cells by regulating miR-490-3p/RSF1 axis. Cancer Biother Radiopharm. 35:351–361. 2020. View Article : Google Scholar : PubMed/NCBI
27	Wu G, Ju X, Wang Y, Li Z and Gan X: Up-regulation of SNHG6 activates SERPINH1 expression by competitive binding to miR-139-5p to promote hepatocellular carcinoma progression. Cell Cycle. 18:1849–1867. 2019. View Article : Google Scholar : PubMed/NCBI
28	Ji D, Wang Y, Li H, Sun B and Luo X: Long non-coding RNA LINC00461/miR-149-5p/LRIG2 axis regulates hepatocellular carcinoma progression. Biochem Biophys Res Commun. 512:176–181. 2019. View Article : Google Scholar : PubMed/NCBI
29	Zhou FC, Zhang YH, Liu HT, Song J and Shao J: lncRNA LINC00588 suppresses the progression of osteosarcoma by acting as a ceRNA for miRNA-1972. Front Pharmacol. 11:2552020. View Article : Google Scholar : PubMed/NCBI
30	Yu Y, Gao F, He Q, Li G and Ding G: lncRNA UCA1 functions as a ceRNA to promote prostate cancer progression via sponging miR143. Mol Ther Nucleic Acids. 19:751–758. 2020. View Article : Google Scholar : PubMed/NCBI
31	Ma Y, Liu Y, Pu YS, Cui ML, Mao ZJ, Li ZZ, He L, Wu M and Wang JH: lncRNA IGFL2-AS1 functions as a ceRNA in regulating ARPP19 through competitive binding to miR-802 in gastric cancer. Mol Carcinog. 59:311–322. 2020. View Article : Google Scholar : PubMed/NCBI
32	Li K, Jiang Y, Xiang X, Gong Q, Zhou C, Zhang L, Ma Q and Zhuang L: Long non-coding RNA SNHG6 promotes the growth and invasion of non-small cell lung cancer by downregulating miR-101-3p. Thorac Cancer. 11:1180–1190. 2020. View Article : Google Scholar : PubMed/NCBI
33	Meng Q, Yang BY, Liu B, Yang JX and Sun Y: Long non-coding RNA SNHG6 promotes glioma tumorigenesis by sponging miR-101-3p. Int J Biol Markers. 33:148–155. 2018. View Article : Google Scholar : PubMed/NCBI
34	Chang L, Yuan Y, Li C, Guo T, Qi H, Xiao Y, Dong X, Liu Z and Liu Q: Upregulation of SNHG6 regulates ZEB1 expression by competitively binding miR-101-3p and interacting with UPF1 in hepatocellular carcinoma. Cancer Lett. 383:183–194. 2016. View Article : Google Scholar : PubMed/NCBI
35	Peng YG, Zhang ZQ, Chen YB and Huang JA: Rap2b promotes proliferation, migration, and invasion of lung cancer cells. J Recept Signal Transduct Res. 36:459–464. 2016. View Article : Google Scholar : PubMed/NCBI
36	Zhang M, Zhuang Q and Cui L: miR-194 inhibits cell proliferation and invasion via repression of RAP2B in bladder cancer. Biomed Pharmacother. 80:268–275. 2016. View Article : Google Scholar : PubMed/NCBI
37	Lv GY, Miao J and Zhang XL: Long noncoding RNA XIST promotes osteosarcoma progression by targeting Ras-Related protein RAP2B via miR-320b. Oncol Res. 26:837–846. 2018. View Article : Google Scholar : PubMed/NCBI