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Macrophages in Giemsa-stained cerebrospinal fluid specimens predict carcinomatous meningitis

  • Authors:
    • Sayaka Kobayashi
    • Masanao Saio
    • Misa Fujimori
    • Junko Hirato
    • Tetsunari Oyama
    • Toshio Fukuda
  • View Affiliations / Copyright

    Affiliations: Laboratory of Histopathology and Cytopathology, Department of Laboratory Sciences, Gunma University Graduate School of Health Sciences, Maebashi, Gunma 371-8514, Japan, Clinical Department of Pathology, Gunma University Hospital, Maebashi, Gunma 371-8511, Japan
    Copyright: © Kobayashi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 352
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    Published online on: October 11, 2020
       https://doi.org/10.3892/ol.2020.12217
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Abstract

Carcinomatous meningitis is a condition in which tumor cells spread to the subarachnoid space. Leukocyte counting and typing of cerebrospinal fluid (CSF) cell components are performed manually or using flow cytometry. However, a detailed analysis of these variables using cytological specimens has not yet been reported. The present study analyzed cytological specimens using Giemsa staining and whole slide imaging with computer-assisted image analysis (CAIA) to clarify the characteristics of the leukocyte population in CSF, especially in carcinomatous meningitis. Manual evaluation was performed using 280 Giemsa-stained cytological CSF specimens. For 49 samples, CAIA was used for the whole area of Papanicolaou (Pap) staining, and Giemsa-stained specimens of the same samples were imaged using a virtual slide scanner. The nuclear morphology of the leukocytes was assessed, and the total leukocyte and leukocyte subset (lymphocytes, neutrophils and macrophages) counts were evaluated. Then, the number and percentage of each leukocyte subset population were evaluated. The total leukocyte count was significantly higher in Giemsa-stained specimens compared with in Pap-stained specimens. The percentage of macrophages was significantly higher in samples from patients with non-hematological tumors compared with in samples from patients without tumors, which was confirmed by manual evaluation of the specimens. In addition, the cut-off value of the percentage of macrophages that could discriminate between the tumor history negative cases and cytologically tumor positive cases was determined, revealing that a higher proportion of macrophages reflected the existence of atypical/malignant epithelial tumor cells in CSF samples. Thus, atypical cell screening and analysis of the background characteristics of the leukocyte population should be the focus of cytological specimen screening, especially not to miss carcinomatous meningitis.
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Copy and paste a formatted citation
Spandidos Publications style
Kobayashi S, Saio M, Fujimori M, Hirato J, Oyama T and Fukuda T: Macrophages in Giemsa-stained cerebrospinal fluid specimens predict carcinomatous meningitis. Oncol Lett 20: 352, 2020.
APA
Kobayashi, S., Saio, M., Fujimori, M., Hirato, J., Oyama, T., & Fukuda, T. (2020). Macrophages in Giemsa-stained cerebrospinal fluid specimens predict carcinomatous meningitis. Oncology Letters, 20, 352. https://doi.org/10.3892/ol.2020.12217
MLA
Kobayashi, S., Saio, M., Fujimori, M., Hirato, J., Oyama, T., Fukuda, T."Macrophages in Giemsa-stained cerebrospinal fluid specimens predict carcinomatous meningitis". Oncology Letters 20.6 (2020): 352.
Chicago
Kobayashi, S., Saio, M., Fujimori, M., Hirato, J., Oyama, T., Fukuda, T."Macrophages in Giemsa-stained cerebrospinal fluid specimens predict carcinomatous meningitis". Oncology Letters 20, no. 6 (2020): 352. https://doi.org/10.3892/ol.2020.12217
Copy and paste a formatted citation
x
Spandidos Publications style
Kobayashi S, Saio M, Fujimori M, Hirato J, Oyama T and Fukuda T: Macrophages in Giemsa-stained cerebrospinal fluid specimens predict carcinomatous meningitis. Oncol Lett 20: 352, 2020.
APA
Kobayashi, S., Saio, M., Fujimori, M., Hirato, J., Oyama, T., & Fukuda, T. (2020). Macrophages in Giemsa-stained cerebrospinal fluid specimens predict carcinomatous meningitis. Oncology Letters, 20, 352. https://doi.org/10.3892/ol.2020.12217
MLA
Kobayashi, S., Saio, M., Fujimori, M., Hirato, J., Oyama, T., Fukuda, T."Macrophages in Giemsa-stained cerebrospinal fluid specimens predict carcinomatous meningitis". Oncology Letters 20.6 (2020): 352.
Chicago
Kobayashi, S., Saio, M., Fujimori, M., Hirato, J., Oyama, T., Fukuda, T."Macrophages in Giemsa-stained cerebrospinal fluid specimens predict carcinomatous meningitis". Oncology Letters 20, no. 6 (2020): 352. https://doi.org/10.3892/ol.2020.12217
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