Open Access

Nidogen 1 regulates proliferation and migration/invasion in murine claudin‑low mammary tumor cells

  • Authors:
    • Rebecca Jagroop
    • Courtney J. Martin
    • Roger A. Moorehead
  • View Affiliations

  • Published online on: November 18, 2020     https://doi.org/10.3892/ol.2020.12313
  • Article Number: 52
  • Copyright: © Jagroop et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Nidogen 1 (NID1) is a glycoprotein found in basement membranes involved in cross‑linking collagen IV and laminin. The role of NID in breast cancer has only been evaluated in a small number of studies and the findings of these studies have been inconsistent. Our previous work revealed that highly tumorigenic murine mammary tumor cells express high levels of Nid1 while weakly tumorigenic mammary tumor cells express low levels of Nid1. To investigate Nid1, two stable knockdown lines were created, and Nid1 knockdown was confirmed at both the mRNA and protein level. Nid1 knockdown significantly reduced cell proliferation and migration/invasion and these reductions in proliferation and migration/invasion could be rescued by conditioned media containing NID1 protein. The reduced migration/invasion observed in the Nid1 knockdown cells was not associated with significant alterations in the epithelial gene Cdh1 or the mesenchymal genes Snai1, Snai2, Twist1, Twist2, Zeb1 and Zeb2. Therefore, suppression of Nid1 expression reduces proliferation and migration/invasion in claudin‑low murine mammary tumor cells.
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Spandidos Publications style
Jagroop R, Martin CJ and Moorehead RA: Nidogen 1 regulates proliferation and migration/invasion in murine claudin‑low mammary tumor cells. Oncol Lett 21: 52, 2021.
APA
Jagroop, R., Martin, C.J., & Moorehead, R.A. (2021). Nidogen 1 regulates proliferation and migration/invasion in murine claudin‑low mammary tumor cells. Oncology Letters, 21, 52. https://doi.org/10.3892/ol.2020.12313
MLA
Jagroop, R., Martin, C. J., Moorehead, R. A."Nidogen 1 regulates proliferation and migration/invasion in murine claudin‑low mammary tumor cells". Oncology Letters 21.1 (2021): 52.
Chicago
Jagroop, R., Martin, C. J., Moorehead, R. A."Nidogen 1 regulates proliferation and migration/invasion in murine claudin‑low mammary tumor cells". Oncology Letters 21, no. 1 (2021): 52. https://doi.org/10.3892/ol.2020.12313