Nidogen 1 regulates proliferation and migration/invasion in murine claudin‑low mammary tumor cells

Jagroop,Rebecca; Martin,Courtney J.; Moorehead,Roger A.

doi:10.3892/ol.2020.12313

Nidogen 1 regulates proliferation and migration/invasion in murine claudin‑low mammary tumor cells

Authors:
- Rebecca Jagroop
- Courtney J. Martin
- Roger A. Moorehead
View Affiliations

Affiliations: Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, ON N1G2W1, Canada
Published online on: November 18, 2020 https://doi.org/10.3892/ol.2020.12313
Article Number: 52
Copyright: © Jagroop et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Nidogen 1 (NID1) is a glycoprotein found in basement membranes involved in cross‑linking collagen IV and laminin. The role of NID in breast cancer has only been evaluated in a small number of studies and the findings of these studies have been inconsistent. Our previous work revealed that highly tumorigenic murine mammary tumor cells express high levels of Nid1 while weakly tumorigenic mammary tumor cells express low levels of Nid1. To investigate Nid1, two stable knockdown lines were created, and Nid1 knockdown was confirmed at both the mRNA and protein level. Nid1 knockdown significantly reduced cell proliferation and migration/invasion and these reductions in proliferation and migration/invasion could be rescued by conditioned media containing NID1 protein. The reduced migration/invasion observed in the Nid1 knockdown cells was not associated with significant alterations in the epithelial gene Cdh1 or the mesenchymal genes Snai1, Snai2, Twist1, Twist2, Zeb1 and Zeb2. Therefore, suppression of Nid1 expression reduces proliferation and migration/invasion in claudin‑low murine mammary tumor cells.

View Figures

View References

1	Jayadev R and Sherwood DR: Basement membranes. Curr Biol. 27:R207–R211. 2017. View Article : Google Scholar : PubMed/NCBI
2	LeBleu VS, Macdonald B and Kalluri R: Structure and function of basement membranes. Exp Biol Med (Maywood). 232:1121–1129. 2007. View Article : Google Scholar : PubMed/NCBI
3	Chang J and Chaudhuri O: Beyond proteases: Basement membrane mechanics and cancer invasion. J Cell Biol. 218:2456–2469. 2019. View Article : Google Scholar : PubMed/NCBI
4	Yurchenco PD: Basement membranes: cell scaffoldings and signaling platforms. Cold Spring Harb Perspect Biol. 3:a0049112011. View Article : Google Scholar : PubMed/NCBI
5	Alečković M, Wei Y, LeRoy G, Sidoli S, Liu DD, Garcia BA and Kang Y: Identification of Nidogen 1 as a lung metastasis protein through secretome analysis. Genes Dev. 31:1439–1455. 2017. View Article : Google Scholar : PubMed/NCBI
6	Martino-Echarri E, Fernández-Rodríguez R, Rodríguez-Baena FJ, Barrientos-Durán A, Torres-Collado AX, Plaza-Calonge Mdel C, Amador-Cubero S, Cortés J, Reynolds LE, Hodivala-Dilke KM and Rodríguez-Manzaneque JC: Contribution of ADAMTS1 as a tumor suppressor gene in human breast carcinoma. Linking its tumor inhibitory properties to its proteolytic activity on nidogen-1 and nidogen-2. Int J Cancer. 133:2315–2324. 2013. View Article : Google Scholar : PubMed/NCBI
7	Ferraro DA, Patella F, Zanivan S, Donato C, Aceto N, Giannotta M, Dejana E, Diepenbruck M, Christofori G and Buess M: Endothelial cell-derived nidogen-1 inhibits migration of SK-BR-3 breast cancer cells. BMC Cancer. 19:3122019. View Article : Google Scholar : PubMed/NCBI
8	Watson KL, Jones RA, Bruce A and Moorehead RA: The miR-200b/200a/429 cluster prevents metastasis and induces dormancy in a murine claudin-low mammary tumor cell line. Exp Cell Res. 369:17–26. 2018. View Article : Google Scholar : PubMed/NCBI
9	Jones R, Watson K, Bruce A, Nersesian S, Kitz J and Moorehead R: Re-expression of miR-200c suppresses proliferation, colony formation and in vivo tumor growth of murine claudin-low mammary tumor cells. Oncotarget. 8:23727–23749. 2017. View Article : Google Scholar : PubMed/NCBI
10	Campbell CI and Moorehead RA: Mammary tumors that become independent of the type I insulin-like growth factor receptor express elevated levels of platelet-derived growth factor receptors. BMC Cancer. 11:4802011. View Article : Google Scholar : PubMed/NCBI
11	Campbell CI, Thompson DE, Siwicky MD and Moorehead RA: Murine mammary tumor cells with a claudin-low genotype. Cancer Cell Int. 11:282011. View Article : Google Scholar : PubMed/NCBI
12	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
13	Chorner PM and Moorehead RA: A-674563, a putative AKT1 inhibitor that also suppresses CDK2 activity, inhibits human NSCLC cell growth more effectively than the pan-AKT inhibitor, MK-2206. PLoS One. 13:e01933442018. View Article : Google Scholar : PubMed/NCBI
14	Dedhar S, Jewell K, Rojiani M and Gray V: The receptor for the basement membrane glycoprotein entactin is the integrin alpha 3/beta 1. J Biol Chem. 267:18908–18914. 1992.PubMed/NCBI
15	Dong LJ, Hsieh JC and Chung AE: Two distinct cell attachment sites in entactin are revealed by amino acid substitutions and deletion of the RGD sequence in the cysteine-rich epidermal growth factor repeat 2. J Biol Chem. 270:15838–15843. 1995. View Article : Google Scholar : PubMed/NCBI
16	Yi XY, Wayner EA, Kim Y and Fish AJ: Adhesion of cultured human kidney mesangial cells to native entactin: Role of integrin receptors. Cell Adhes Commun. 5:237–248. 1998. View Article : Google Scholar : PubMed/NCBI
17	Rokavec M, Bouznad N and Hermeking H: Paracrine induction of epithelial-mesenchymal transition between colorectal cancer cells and its suppression by a p53/miR-192/215/NID1 axis. Cell Mol Gastroenterol Hepatol. 7:783–802. 2019. View Article : Google Scholar : PubMed/NCBI
18	Zhou Y, Zhu Y, Fan X, Zhang C, Wang Y, Zhang L, Zhang H, Wen T, Zhang K, Huo X, et al: NID1, a new regulator of EMT required for metastasis and chemoresistance of ovarian cancer cells. Oncotarget. 8:33110–33121. 2017. View Article : Google Scholar : PubMed/NCBI