Circulating exosomal miR‑125a‑5p as a novel biomarker for cervical cancer

Lv,Aixia; Tu,Zengrong; Huang,Yunhua; Lu,Weiying; Xie,Baoguo

doi:10.3892/ol.2020.12316

Circulating exosomal miR‑125a‑5p as a novel biomarker for cervical cancer

Authors:
- Aixia Lv
- Zengrong Tu
- Yunhua Huang
- Weiying Lu
- Baoguo Xie
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Affiliations: Reproductive Center, The 940th Hospital of Joint Logistics Support Force of Chinese People's Liberation Army, Lanzhou, Gansu 730050, P.R. China, Reproductive Center, The First Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China, Reproductive Medicine Center, Hainan Maternal and Children's Medical Center, Haikou, Hainan 570206, P.R. China, Reproductive Medicine Center, The First Affiliated Hospital of Hainan Medical University, Haikou, Hainan 570102, P.R. China
Published online on: November 18, 2020 https://doi.org/10.3892/ol.2020.12316
Article Number: 54
Copyright: © Lv et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Exosomal microRNAs (miRs/miRNAs) have been reported to be associated with cervical cancer. The aim of the present study was to investigate circulating exosomal miRNA as a biomarker for cervical cancer diagnosis. In the present study, samples from 6 patients with cervical cancer and 6 healthy control subjects were retrieved for exosomal RNA‑sequencing. The results revealed that a total of 39 miRNAs were differentially expressed between patients with cervical cancer and healthy controls (P<0.001; fold‑change >2.0). Exosomal miR‑125a‑5p was further quantified in plasma from 60 subjects, which included 22 healthy individuals and 38 patients with cervical cancer. miR‑16a‑5p served as the reference miRNA for quantitative PCR analysis of exosomal miR‑125a‑5p in patients with cervical cancer and healthy individuals. The results revealed that exosomal miR‑125a‑5p expression levels in the patients with cervical cancer were significantly lower than those in the healthy controls (P<0.001). Receiver operating characteristic (ROC) curve analyses were performed and the results revealed that the level of plasma exosomal miR‑125a‑5p was a potential marker for differentiating between non‑cervical cancer and cervical cancer, with an ROC area under the curve of 0.7129. At the cut‑off value of 2.537 for miR‑125a‑5p, cervical cancer diagnostic sensitivities and specificities were 59.1 and 84.2%, respectively. The present study provides confirmation that exosomal miR‑125a‑5p could potentially serve as a biomarker for cervical cancer diagnosis. The present study involved only a small number of clinical samples; more samples are required to support the conclusions of the present study.

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