Identification of key genes in lung adenocarcinoma based on a competing endogenous RNA network
- Zikun Song
- Yinjiang Zhang
- Zheren Chen
- Bicheng Zhang
Affiliations: Department of Intensive Care Medicine, The People's Second Hospital of Liaocheng, Linqing, Shandong 252601, P.R. China, School of Pharmacy, Minzu University of China, Beijing 100081, P.R. China, Department of Oncology, Renmin Hospital of Shishou, Jingzhou, Hubei 434400, P.R. China, Department of Oncology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430070, P.R. China
- Published online on: November 19, 2020 https://doi.org/10.3892/ol.2020.12322
Copyright : © Song
et al. This is an open access article distributed under the
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Commons Attribution License [CC BY 4.0].
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Lung adenocarcinoma (LUAD) is the most commonly diagnosed type of lung cancer and exhibits a high morbidity. The present study aimed to investigate the long non‑coding RNA (lncRNA)‑associated competing endogenous RNA (ceRNA) mechanisms in LUAD. The receptor activity modifying protein 2‑antisense RNA 1 (RAMP2‑AS1) was identified using GSE113852 and GSE130779 datasets downloaded from the Gene Expression Omnibus database, and the downregulation of RAMP2‑AS1 was the most significant in LUAD. In addition, microRNA (miR)‑296‑5p was identified to bind to RAMP2‑AS1 via bioinformatics analysis. Subsequently, CD44, cyclin D3 (CCND3), neurocalcin δ (NCALD), microtubule actin crosslinking factor 1 (MACF1) and potassium channel tetramerization domain containing 15 were obtained by intersecting the predicted target genes of miR‑296‑5p and 368 differentially expressed mRNAs in LUAD. According to the Gene Expression Profiling Interactive Analysis and UALCAN databases, these five mRNAs were downregulated in LUAD, and their expression levels were positively correlated with those of RAMP2‑AS1. CD44, CCND3, NCALD and MACF1 were selected as key mRNAs in LUAD based on prognostic analyses. Furthermore, functional enrichment analyses were performed and an interaction network was constructed to reveal the functions of the RAMP2‑AS1‑associated ceRNA in LUAD. The results indicated that the functions were mainly enriched in generic transcription pathways, cyclin D‑associated events in G1 and epithelial stromal transformation. Reverse transcription‑quantitative PCR assays revealed that RAMP2‑AS1, CD44, CCND3, NCALD and MACF1 expression was lower in tumor tissues than in normal tissues, while miR‑296‑5p expression was higher in tumor tissues compared with in normal tissues. The association between RAMP2‑AS1 and MACF1 was further confirmed using in vitro experiments. Overall, the present results indicated that RAMP2‑AS1, miR‑296‑5p, CD44, CCND3, NCALD and MACF1 may be involved in LUAD progression and may therefore serve as potential biomarkers and provide a theoretical basis for the study of the pathogenesis of LUAD.