The expression of trefoil factor 3 is related to histologic subtypes and invasiveness in lung adenocarcinoma

Luo,Wenjuan; Tahara,Shinichiro; Kawasaki,Keisuke; Kobayashi,Ayaka; Nojima,Satoshi; Morii,Eiichi

doi:10.3892/ol.2020.12325

January-2021 Volume 21 Issue 1

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January-2021 Volume 21 Issue 1

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The expression of trefoil factor 3 is related to histologic subtypes and invasiveness in lung adenocarcinoma

Authors:
- Wenjuan Luo
- Shinichiro Tahara
- Keisuke Kawasaki
- Ayaka Kobayashi
- Satoshi Nojima
- Eiichi Morii
View Affiliations / Copyright

Affiliations: Department of Pathology, Osaka University Graduate School of Medicine, Osaka 565‑0871, Japan

Copyright: © Luo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Article Number: 63
|
Published online on: November 19, 2020

https://doi.org/10.3892/ol.2020.12325
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Abstract

Adenocarcinoma is the most common histological type of lung cancer and has various histologic subtypes, including lepidic, papillary, acinar and invasive mucinous adenocarcinoma. Histologic subtypes are associated with tumor invasiveness. For example, the lepidic subtype is less invasive than the papillary/acinar subtype. Trefoil factor 3 (TFF3) is a small secreting protein that is a member of the trefoil factor family, which is involved in mucosal stabilization and repair through its mitogenic and antiapoptotic activities. TFF3 overexpression is associated with various types of cancer. In lung cancer, TFF3 is expressed significantly in adenocarcinoma. However, the relationship between TFF3 expression and histologic subtypes in lung adenocarcinoma is unclear. The current study immunohistochemically revealed that, beside invasive mucinous carcinoma, the expression of TFF3 in papillary and acinar adenocarcinoma was significantly higher than that in lepidic adenocarcinoma. To further confirm these results, the expression of TFF3 in cases with both lepidic and papillary/acinar areas were examined. The expression of TFF3 in papillary/acinar areas was significantly higher when compared with lepidic areas in a single sample. Furthermore, using the lung adenocarcinoma cell line A549, TFF3‑knockdown cells were generated. The results revealed that knockdown of TFF3 attenuated invasion. In vitro and immunohistochemical assays using clinical samples demonstrated that TFF3 expression was associated with lung adenocarcinoma invasiveness. To the best of our knowledge, the current study is the first to report that TFF3 expression was associated with the histologic subtypes of lung adenocarcinoma.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

The expression of trefoil factor 3 is related to histologic subtypes and invasiveness in lung adenocarcinoma

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