Open Access

Long non‑coding RNA AK001903 regulates tumor progression in cervical cancer

  • Authors:
    • Guanglei Zhong
    • Xingyu Fang
    • Qingsheng Xie
    • Yuhan  Wang
    • Zhongqiu Lin
    • Rongchun Lin
    • Tingting Yao
  • View Affiliations

  • Published online on: November 30, 2020     https://doi.org/10.3892/ol.2020.12338
  • Article Number: 77
  • Copyright: © Zhong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

A majority of cervical cancers are squamous cell carcinomas, arising from the squamous (flattened) epithelial cells that line the cervix. Long noncoding RNAs (lncRNAs) are a unique class of messenger RNA‑like transcripts of at least 200 nucleotides in length with no significant protein‑coding capacity. Aberrant lncRNA expression is emerging as a major component of the cancer transcriptome. In the present study, lncRNA microarrays were conducted to investigate the differentially expression lncRNAs in cervical cancer (CC) tissues compared with peritumoral tissues. Then, the most significantly upregulated lncRNA, which was lncRNA‑AK001903 was selected to conduct further experiments. Real‑time Quantitative polymerase chain reaction was conducted to investigate lncRNA‑AK001903 expression in CC tissues and Hela, Siha, Ca Ski, C33a, H8 (HPV‑immortalized cervical epithelial cell line) cell lines, and in situ hybridization histochemistry (ISHH) was performed to detect lncRNA‑AK001903 expression level in different CC stages. The effect of lncRNA‑AK001903 on cell proliferation, invasion and migration was assessed after knockdown of lncRNA‑AK001903. The findings of the study confirmed that lncRNA‑AK001903 was upregulated in CC cells and tissues compared with normal cell line H8 and peritumoral tissues. ISHH demonstrated that the expression level of lncRNA‑AK001903 was connected with International Federation of Gynecology and Obstetrics (2018) stage of CC. Knockdown of lncRNA‑AK001903 inhibited cell proliferation, invasion and migration in Ca Ski cells. In conclusion, lncRNA‑AK001903 was demonstrated to be an oncogenic lncRNA that promotes tumor progression and may be an effective target for CC treatment in the near future.
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February-2021
Volume 21 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Zhong G, Fang X, Xie Q, Wang Y, Lin Z, Lin R and Yao T: Long non‑coding RNA AK001903 regulates tumor progression in cervical cancer. Oncol Lett 21: 77, 2021
APA
Zhong, G., Fang, X., Xie, Q., Wang, Y., Lin, Z., Lin, R., & Yao, T. (2021). Long non‑coding RNA AK001903 regulates tumor progression in cervical cancer. Oncology Letters, 21, 77. https://doi.org/10.3892/ol.2020.12338
MLA
Zhong, G., Fang, X., Xie, Q., Wang, Y., Lin, Z., Lin, R., Yao, T."Long non‑coding RNA AK001903 regulates tumor progression in cervical cancer". Oncology Letters 21.2 (2021): 77.
Chicago
Zhong, G., Fang, X., Xie, Q., Wang, Y., Lin, Z., Lin, R., Yao, T."Long non‑coding RNA AK001903 regulates tumor progression in cervical cancer". Oncology Letters 21, no. 2 (2021): 77. https://doi.org/10.3892/ol.2020.12338