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Myeloid and plasmacytoid dendritic cell combined vaccines loaded with heat‑treated tumor cell lysates enhance antitumor activity in murine lung cancer

  • Authors:
    • Huiguo Chen
    • Jianfeng Tan
    • Xiaojun Li
    • Hui Li
    • Weibin Wu
    • Yonghui Wu
    • Jian Zhang
    • Lijia Gu
  • View Affiliations / Copyright

    Affiliations: Department of Cardiothoracic Surgery, The Third Affiliated Hospital, Sun Yat‑sen University, Tianhe, Guangzhou, Guangdong 510630, P.R. China, Department of Thoracic Surgery, ShenZhen Hospital of Southern Medical University, Bao'an, ShenZhen, Guangdong 518000, P.R. China, Department of Cardiothoracic Surgery, ZhuJiang Hospital of Southern Medical University, Haizhu, Guangzhou, Guangdong 510280, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 90
    |
    Published online on: December 6, 2020
       https://doi.org/10.3892/ol.2020.12351
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Abstract

The present study aimed to investigate the efficacy of a myeloid dendritic cell (mDCs) and plasmacytoid (p)DC combined vaccine loaded with heat‑treated cancer cell lysates against lung cancer cells. The mDCs and pDCs were selected using magnetic bead sorting. Antigen loading was performed by adding heat‑treated Lewis lung cancer cell lysates to mDC, pDC or mDC+pDC (1:1). Surface expression of CD80, CD86, CD40 and major histocompatibility complex (MHC)‑II molecules were determined using flow cytometry, and the secretion of cytokines IL‑12, IL‑6 and TNF‑α were assessed using ELISA assays. The effect of the mDC and pDC vaccine on cytotoxic T lymphocytes (CTLs) against tumor cells was investigated. Tumor‑bearing nude mice were intravenously injected with the mDC and pDC combined vaccine. Tumor tissues were collected for hematoxylin and eosin and TUNEL staining. Loading with tumor cell lysate significantly upregulated the surface expression of costimulatory molecules MHC‑II on DCs and enhanced secretions of IL‑6, IL‑12 and TNF‑α by DCs. In addition, the tumor cell lysate‑loaded mDC and pDC combined vaccine significantly promoted lymphocyte proliferation and enhanced CTL‑mediated cytotoxicity against Lewis lung cancer cells compared with mDC or pDC treatment alone. Furthermore, intravenous injection of the mDC and pDC combined vaccine into tumor‑bearing nude mice significantly inhibited subcutaneous tumor growth and induced necrosis and apoptosis within the tumor tissue. Overall, the pDC and mDC combination vaccine loaded with heat‑treated Lewis lung cancer cell lysate had a synergistic effect on the induction of T lymphocyte proliferation and antitumor efficacy, which may be associated with the upregulation of co‑stimulatory molecules and cytokine secretions.
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1 

Mellstedt H, Vansteenkiste J and Thatcher N: Vaccines for the treatment of non-small cell lung cancer: Investigational approaches and clinical experience. Lung Cancer. 73:11–17. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Hirsch FR, Scagliotti GV, Mulshine JL, Kwon R, Curran WJ, Wu YL and Paz-Ares L: Lung cancer: Current therapies and new targeted treatments. Lancet. 389:299–311. 2017. View Article : Google Scholar : PubMed/NCBI

3 

Gardner A and Ruffell B: Dendritic cells and cancer immunity. Trends Immunol. 37:855–865. 2016. View Article : Google Scholar : PubMed/NCBI

4 

González FE, Gleisner A, Falcón-Beas F, Osorio F, López MN and Salazar-Onfray F: Tumor cell lysates as immunogenic sources for cancer vaccine design. Hum Vaccines Immunother. 10:3261–3269. 2014. View Article : Google Scholar

5 

Ilett E, Prestwich R and Melcher A: The evolving role of dendritic cells in cancer therapy. Expert Opin Biol Ther. 10:369–379. 2010. View Article : Google Scholar : PubMed/NCBI

6 

Bol KF, Schreibelt G, Gerritsen WR, De Vries IJM and Figdor CG: Dendritic cell-based immunotherapy: State of the art and beyond. Clin Cancer Res. 22:1897–1906. 2016. View Article : Google Scholar : PubMed/NCBI

7 

Kadowaki N, Ho S, Antonenko S, Malefyt R, Kastelein R, Bazan F and Liu Y: Subsets of human dendritic cell precursors express different toll-like receptors and respond to different microbial antigens. J Exp Med. 194:863–869. 2001. View Article : Google Scholar : PubMed/NCBI

8 

Swiecki M and Colonna M: The multifaceted biology of plasmacytoid dendritic cells. Nat Rev Immunol. 15:471–485. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Nizzoli G, Krietsch J, Weick A, Steinfelder S, Facciotti F, Gruarin P, Bianco A, Steckel B, Moro M, Crosti M, et al: Human CD1c+ dendritic cells secrete high levels of IL-12 and potently prime cytotoxic T-cell responses. Blood. 122:932–942. 2013. View Article : Google Scholar : PubMed/NCBI

10 

Lou Y, Liu C, Kim GJ, Liu YJ, Hwu P and Wang G: Plasmacytoid dendritic cells synergize with myeloid dendritic cells in the induction of antigen-specific antitumor immune responses. J Immunol. 178:1534–1541. 2007. View Article : Google Scholar : PubMed/NCBI

11 

Mocellin S, Mandruzzato S, Bronte V, Lise M and Nitti D: Part I: Vaccines for solid tumours. Lancet Oncol. 5:681–689. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Schuler G, Schuler-Thurner B and Steinman RM: The use of dendritic cells in cancer immunotherapy. Curr Opin Immunol. 15:138–147. 2003. View Article : Google Scholar : PubMed/NCBI

13 

Tagliamonte M, Petrizzo A, Tornesello ML, Buonaguro FM and Buonaguro L: Antigen-specific vaccines for cancer treatment. Hum Vaccines Immunother. 10:3332–3346. 2014. View Article : Google Scholar

14 

Ridolfi R, Petrini M, Fiammenghi L, Stefanelli M, Ridolfi L, Ballardini M, Migliori G and Riccobon A: Improved overall survival in dendritic cell vaccination-induced immunoreactive subgroup of advanced melanoma patients. J Transl Med. 4:362006. View Article : Google Scholar : PubMed/NCBI

15 

Trefzer U, Herberth G, Wohlan K, Milling A, Thiemann M, Sharav T, Sparbier K, Sterry W and Walden P: Tumour-dendritic hybrid cell vaccination for the treatment of patients with malignant melanoma: Immunological effects and clinical results. Vaccine. 23:2367–2373. 2005. View Article : Google Scholar : PubMed/NCBI

16 

Nagayama H, Sato K, Morishita M, Uchimaru K, Oyaizu N, Inazawa T, Yamasaki T, Enomoto M, Nakaoka T, Nakamura T, et al: Results of a phase I clinical study using autologous tumour lysate-pulsed monocyte-derived mature dendritic cell vaccinations for stage IV malignant melanoma patients combined with low dose interleukin-2. Melanoma Res. 13:521–530. 2003. View Article : Google Scholar : PubMed/NCBI

17 

Hersey P, Menzies SW, Halliday GM, Nguyen T, Farrelly ML, DeSilva C and Lett M: Phase I/II study of treatment with dendritic cell vaccines in patients with disseminated melanoma. Cancer Immunol Immunother. 53:125–134. 2004. View Article : Google Scholar : PubMed/NCBI

18 

Jouanneau E, Poujol D, Gulia S, Le Mercier I, Blay JY, Belin MF and Puisieux I: Dendritic cells are essential for priming but inefficient for boosting antitumour immune response in an orthotopic murine glioma model. Cancer Immunol Immunother. 55:254–267. 2006. View Article : Google Scholar : PubMed/NCBI

19 

Zhang Y, Yoneyama H, Wang Y, Ishikawa S, Hashimoto S, Gao JL, Murphy P and Matsushima K: Mobilization of dendritic cell precursors into the circulation by administration of MIP-1alpha in mice. J Natl Cancer Inst. 96:201–209. 2004. View Article : Google Scholar : PubMed/NCBI

20 

Hatfield P, Merrick AE, West E, ODonnell D, Selby P, Vile R and Melcher AA: Optimization of dendritic cell loading with tumor cell lysates for cancer immunotherapy. J Immunother. 31:620–632. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Kim HS, Choo YS, Koo T, Bang S, Oh TY, Wen J and Song SY: Enhancement of antitumor immunity of dendritic cells pulsed with heat-treated tumor lysate in murine pancreatic cancer. Immunol Lett. 103:142–148. 2006. View Article : Google Scholar : PubMed/NCBI

22 

Qiu J, Li GW, Sui YF, Song HP, Si SY and Ge W: Heat-shocked tumor cell lysate-pulsed dendritic cells induce effective anti-tumor immune response in vivo. World J Gastroenterol. 12:473–478. 2006. View Article : Google Scholar : PubMed/NCBI

23 

Castelli C, Rivoltini L, Rini F, Belli F, Testori A, Maio M, Mazzaferro V, Coppa J, Srivastava PK and Parmiani G: Heat shock proteins: Biological functions and clinical application as personalized vaccines for human cancer. Cancer Immunol Immunother. 53:227–233. 2004. View Article : Google Scholar : PubMed/NCBI

24 

Pelleitier M and Montplaisir S: The nude mouse: A model of deficient T-cell function. Methods Achiev Exp Pathol. 7:149–166. 1975.PubMed/NCBI

25 

Alberts B, Johnson A and Lewis J: Helper T cells and lymphocyte activation. Molecular Biology of the Cell. 1–8. 2002.PubMed/NCBI

26 

Zhang Y, Zhang N, Zhao M and Hoffman RM: Comparison of the selective targeting efficacy of Salmonella typhimurium A1-R and VNP20009 on the Lewis lung carcinoma in nude mice. Oncotarget. 6:14625–14631. 2015. View Article : Google Scholar : PubMed/NCBI

27 

Fu Q, Wu Y, Yan F, Wang N, Wang W, Cao X, Wang Y and Wan T: Efficient induction of a Her2-specific anti-tumor response by dendritic cells pulsed with a Hsp70L1-Her2 341–456 fusion protein. Cell Mol Immunol. 8:424–432. 2011. View Article : Google Scholar : PubMed/NCBI

28 

Song S, Zhang K, You H, Wang J, Wang Z, Yan C and Liu F: Significant anti-tumour activity of adoptively transferred T cells elicited by intratumoral dendritic cell vaccine injection through enhancing the ratio of CD8+ T cell/regulatory T cells in tumour. Clin Exp Immunol. 162:75–83. 2010. View Article : Google Scholar : PubMed/NCBI

29 

Piccioli D, Tavarini S, Borgogni E, Steri V, Nuti S, Sammicheli C, Bardelli M, Montagna D, Locatelli F and Wack A: Functional specialization of human circulating CD16 and CD1c myeloid dendritic-cell subsets. Blood. 109:5371–5379. 2007. View Article : Google Scholar : PubMed/NCBI

30 

Audiger C, Rahman MJ, Yun TJ, Tarbell KV and Lesage S: The importance of dendritic cells in maintaining immune tolerance. J Immunol. 198:2223–2231. 2017. View Article : Google Scholar : PubMed/NCBI

31 

Liu J, Cao S, Kim S, Chung EY, Homma Y, Guan X, Jimenez V and Ma X: Interleukin-12: An update on its immunological activities, signaling and regulation of gene expression. Curr Immunol Rev. 1:119–137. 2005. View Article : Google Scholar : PubMed/NCBI

32 

Blanco P, Palucka AK, Pascual V and Banchereau J: Dendritic cells and cytokines in human inflammatory and autoimmune diseases. Cytokine Growth Factor Rev. 19:41–52. 2008. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Chen H, Tan J, Li X, Li H, Wu W, Wu Y, Zhang J and Gu L: Myeloid and plasmacytoid dendritic cell combined vaccines loaded with heat‑treated tumor cell lysates enhance antitumor activity in murine lung cancer. Oncol Lett 21: 90, 2021.
APA
Chen, H., Tan, J., Li, X., Li, H., Wu, W., Wu, Y. ... Gu, L. (2021). Myeloid and plasmacytoid dendritic cell combined vaccines loaded with heat‑treated tumor cell lysates enhance antitumor activity in murine lung cancer. Oncology Letters, 21, 90. https://doi.org/10.3892/ol.2020.12351
MLA
Chen, H., Tan, J., Li, X., Li, H., Wu, W., Wu, Y., Zhang, J., Gu, L."Myeloid and plasmacytoid dendritic cell combined vaccines loaded with heat‑treated tumor cell lysates enhance antitumor activity in murine lung cancer". Oncology Letters 21.2 (2021): 90.
Chicago
Chen, H., Tan, J., Li, X., Li, H., Wu, W., Wu, Y., Zhang, J., Gu, L."Myeloid and plasmacytoid dendritic cell combined vaccines loaded with heat‑treated tumor cell lysates enhance antitumor activity in murine lung cancer". Oncology Letters 21, no. 2 (2021): 90. https://doi.org/10.3892/ol.2020.12351
Copy and paste a formatted citation
x
Spandidos Publications style
Chen H, Tan J, Li X, Li H, Wu W, Wu Y, Zhang J and Gu L: Myeloid and plasmacytoid dendritic cell combined vaccines loaded with heat‑treated tumor cell lysates enhance antitumor activity in murine lung cancer. Oncol Lett 21: 90, 2021.
APA
Chen, H., Tan, J., Li, X., Li, H., Wu, W., Wu, Y. ... Gu, L. (2021). Myeloid and plasmacytoid dendritic cell combined vaccines loaded with heat‑treated tumor cell lysates enhance antitumor activity in murine lung cancer. Oncology Letters, 21, 90. https://doi.org/10.3892/ol.2020.12351
MLA
Chen, H., Tan, J., Li, X., Li, H., Wu, W., Wu, Y., Zhang, J., Gu, L."Myeloid and plasmacytoid dendritic cell combined vaccines loaded with heat‑treated tumor cell lysates enhance antitumor activity in murine lung cancer". Oncology Letters 21.2 (2021): 90.
Chicago
Chen, H., Tan, J., Li, X., Li, H., Wu, W., Wu, Y., Zhang, J., Gu, L."Myeloid and plasmacytoid dendritic cell combined vaccines loaded with heat‑treated tumor cell lysates enhance antitumor activity in murine lung cancer". Oncology Letters 21, no. 2 (2021): 90. https://doi.org/10.3892/ol.2020.12351
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