Upregulation of miR-1307-3p and its function in the clinical prognosis and progression of gastric cancer

Ma,Yanhui; Zhou,Aifeng; Song,Juan

doi:10.3892/ol.2020.12352

Upregulation of miR-1307-3p and its function in the clinical prognosis and progression of gastric cancer

Authors:
- Yanhui Ma
- Aifeng Zhou
- Juan Song
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Affiliations: Department of Laboratory, Qingdao Central Hospital, Qingdao, Shandong 266042, P.R. China
Published online on: December 6, 2020 https://doi.org/10.3892/ol.2020.12352
Article Number: 91
Copyright: © Ma et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Gastric cancer is one of the major causes of cancer-associated mortality worldwide. miR-1307-3p has been demonstrated to serve multiple roles in the development of various types of cancer. The present study aimed to evaluate the expression and functional role of miR-1307-3p in the progression of gastric cancer. The expression of miR-1307-3p in gastric cancer tissues and cell lines was detected by reverse transcription quantitative PCR. Furthermore, the correlation between miR-1307-3p expression and the clinicopathological characteristics and prognosis of patients was evaluated. Cell Counting Kit-8 and Transwell assays were performed to analyze the effects of miR-1307-3p on the proliferation and the migratory and invasive abilities of gastric cancer cells, respectively. Dual-luciferase reporter assay was conducted to reveal the potential underlying mechanism of miR-1307-3p. In gastric cancer tissues and cells, miR-1307-3p expression was significantly upregulated compared with the normal tissues and cell lines. In addition, the expression of miR-1307-3p was associated with the Tumor-Node Metastasis stage of patients. The results from Cox regression analysis demonstrated that miR-1307-3p may serve as an independent predictor for the prognosis of patients with gastric cancer. Furthermore, the upregulation of miR-1307-3p in gastric cancer cell lines significantly promoted the cell proliferation and migratory and invasive abilities by targeting DAB2 interacting protein. In conclusion, the findings from the present study suggested that miR-1307-3p may serve as a tumor promoter of gastric cancer and that miR-1307-3p expression in tumor tissues may be used as a prognostic indicator for patients with gastric cancer.

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1	Siegel R, Ma J, Zou Z and Jemal A: Cancer statistics, 2014. CA Cancer J Clin. 64:9–29. 2014. View Article : Google Scholar : PubMed/NCBI
2	Balakrishnan M, George R, Sharma A and Graham DY: Changing Trends in Stomach Cancer Throughout the World. Curr Gastroenterol Rep. 19:362017. View Article : Google Scholar : PubMed/NCBI
3	Global Burden of Disease Cancer Collaboration, ; Fitzmaurice C, Allen C, Barber RM, Barregard L, Bhutta ZA, Brenner H, Dicker DJ, Chimed-Orchir O, Dandona R, Dandona L, et al: Global, regional, and national cancer incidence, mortality, years of life lost, years lived with disability, and disability-adjusted life-years for 32 cancer groups, 1990 to 2015: A systematic analysis for the Global Burden of Disease Study. JAMA Oncol. 3:524–548. 2017. View Article : Google Scholar : PubMed/NCBI
4	de Martel C, Ferlay J, Franceschi S, Vignat J, Bray F, Forman D and Plummer M: Global burden of cancers attributable to infections in 2008: A review and synthetic analysis. Lancet Oncol. 13:607–615. 2012. View Article : Google Scholar : PubMed/NCBI
5	de Martel C, Forman D and Plummer M: Gastric cancer: Epidemiology and risk factors. Gastroenterol Clin North Am. 42:219–240. 2013. View Article : Google Scholar : PubMed/NCBI
6	Yasui W, Yokozaki H, Fujimoto J, Naka K, Kuniyasu H and Tahara E: Genetic and epigenetic alterations in multistep carcinogenesis of the stomach. J Gastroenterol. 35 (Suppl 12):111–115. 2000.PubMed/NCBI
7	Stock M and Otto F: Gene deregulation in gastric cancer. Gene. 360:1–19. 2005. View Article : Google Scholar : PubMed/NCBI
8	Wang WC and Zhang XF, Peng J, Li XF, Wang AL, Bie YQ, Shi LH, Lin MB and Zhang XF: Survival Mechanisms and Influence Factors of Circulating Tumor Cells. BioMed Res Int. 2018:63047012018. View Article : Google Scholar : PubMed/NCBI
9	Karimi P, Islami F, Anandasabapathy S, Freedman ND and Kamangar F: Gastric cancer: Descriptive epidemiology, risk factors, screening, and prevention. Cancer Epidemiol Biomarkers Prev. 23:700–713. 2014. View Article : Google Scholar : PubMed/NCBI
10	Bartel DP: MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell. 116:281–297. 2004. View Article : Google Scholar : PubMed/NCBI
11	Hoffman Y, Bublik DR, Ugalde AP, Elkon R, Biniashvili T, Agami R, Oren M and Pilpel Y: 3′UTR Shortening Potentiates MicroRNA-Based Repression of Pro-differentiation Genes in Proliferating Human Cells. PLoS Genet. 12:e10058792016. View Article : Google Scholar : PubMed/NCBI
12	Kim S and Jeong S: Mutation hotspots in the β-catenin gene: Lessons from the human cancer genome databases. Mol Cells. 42:8–16. 2019.PubMed/NCBI
13	Bartel DP: MicroRNAs: Target recognition and regulatory functions. Cell. 136:215–233. 2009. View Article : Google Scholar : PubMed/NCBI
14	Ahmed FE: Role of miRNA in carcinogenesis and biomarker selection: A methodological view. Expert Rev Mol Diagn. 7:569–603. 2007. View Article : Google Scholar : PubMed/NCBI
15	Brenner B, Hoshen MB, Purim O, David MB, Ashkenazi K, Marshak G, Kundel Y, Brenner R, Morgenstern S, Halpern M, et al: MicroRNAs as a potential prognostic factor in gastric cancer. World J Gastroenterol. 17:3976–3985. 2011. View Article : Google Scholar : PubMed/NCBI
16	Wei Y, Wang Y, Zang A, Wang Z, Fang G and Hong D: MiR-4766-5p inhibits the development and progression of gastric cancer by targeting NKAP. OncoTargets Ther. 12:8525–8536. 2019. View Article : Google Scholar
17	Chen H, Yang Y, Wang J, Shen D, Zhao J and Yu Q: miR-130b-5p promotes proliferation, migration and invasion of gastric cancer cells via targeting RASAL1. Oncol Lett. 15:6361–6367. 2018.PubMed/NCBI
18	Chen S, Wang L, Yao B, Liu Q and Guo C: miR-1307-3p promotes tumor growth and metastasis of hepatocellular carcinoma by repressing DAB2 interacting protein. Biomed Pharmacother. 117:1090552019. View Article : Google Scholar : PubMed/NCBI
19	Han S, Zou H, Lee JW, Han J, Kim HC, Cheol JJ, Kim LS and Kim H: miR-1307-3p stimulates breast cancer development and progression by targeting SMYD4. J Cancer. 10:441–448. 2019. View Article : Google Scholar : PubMed/NCBI
20	Zheng Y, Zheng Y, Lei W, Xiang L and Chen M: miR-1307-3p overexpression inhibits cell proliferation and promotes cell apoptosis by targeting ISM1 in colon cancer. Mol Cell Probes. 48:1014452019. View Article : Google Scholar : PubMed/NCBI
21	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
22	Kalinowski FC, Brown RA, Ganda C, Giles KM, Epis MR, Horsham J and Leedman PJ: microRNA-7: A tumor suppressor miRNA with therapeutic potential. Int J Biochem Cell Biol. 54:312–317. 2014. View Article : Google Scholar : PubMed/NCBI
23	Yadav S, Shekhawat M, Jahagirdar D and Kumar Sharma N: Natural and artificial small RNAs: A promising avenue of nucleic acid therapeutics for cancer. Cancer Biol Med. 14:242–253. 2017. View Article : Google Scholar : PubMed/NCBI
24	Shin VY and Chu KM: MiRNA as potential biomarkers and therapeutic targets for gastric cancer. World J Gastroenterol. 20:10432–10439. 2014. View Article : Google Scholar : PubMed/NCBI
25	Qiu T, Wang K, Li X and Jin J: miR-671-5p inhibits gastric cancer cell proliferation and promotes cell apoptosis by targeting URGCP. Exp Ther Med. 16:4753–4758. 2018.PubMed/NCBI
26	Yu T, Gong L, Li W, Zuo Q, Cai D, Mao H, Wang L, Lin J and Xiao B: MiR-30a suppresses metastasis of gastric adenocarcinoma via targeting FAPα. Cancer Biomark. 27:471–484. 2020. View Article : Google Scholar : PubMed/NCBI
27	Ding L, Zhang S, Xu M, Zhang R, Sui P and Yang Q: MicroRNA-27a contributes to the malignant behavior of gastric cancer cells by directly targeting PH domain and leucine-rich repeat protein phosphatase 2. J Exp Clin Cancer Res. 36:452017. View Article : Google Scholar : PubMed/NCBI
28	Nakamura S, Kanda M and Kodera Y: Incorporating molecular biomarkers into clinical practice for gastric cancer. Expert Rev Anticancer Ther. 19:757–771. 2019. View Article : Google Scholar : PubMed/NCBI
29	Li L, Mou YP, Wang YY, Wang HJ and Mou XZ: miR-199a-3p targets ETNK1 to promote invasion and migration in gastric cancer cells and is associated with poor prognosis. Pathol Res Pract. 215:1525112019. View Article : Google Scholar : PubMed/NCBI
30	Sun L, Yao Y, Lu T, Shang Z, Zhan S, Shi W, Pan G, Zhu X and He S: DAB2IP downregulation enhances the proliferation and metastasis of human gastric cancer cells by derepressing the ERK1/2 pathway. Gastroenterol Res Pract. 2018:29682522018. View Article : Google Scholar : PubMed/NCBI
31	Liu L, Xu C, Hsieh JT, Gong J and Xie D: DAB2IP in cancer. Oncotarget. 7:3766–3776. 2016. View Article : Google Scholar : PubMed/NCBI
32	Nakamura R, Omori T, Mayanagi S, Irino T, Wada N, Kawakubo H, Kameyama K and Kitagawa Y: Risk of lymph node metastasis in undifferentiated-type mucosal gastric carcinoma. World J Surg Oncol. 17:322019. View Article : Google Scholar : PubMed/NCBI
33	Mikami K, Hirano Y, Futami K and Maekawa T: Expansion of lymph node metastasis in mixed-type submucosal invasive gastric cancer. Asian J Surg. 41:462–466. 2018. View Article : Google Scholar : PubMed/NCBI
34	Bausys R, Bausys A, Vysniauskaite I, Maneikis K, Klimas D, Luksta M, Strupas K and Stratilatovas E: Risk factors for lymph node metastasis in early gastric cancer patients: Report from Eastern Europe country- Lithuania. BMC Surg. 17:1082017. View Article : Google Scholar : PubMed/NCBI