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Inhibition of autophagy enhances apoptosis induced by bortezomib in AML cells

  • Authors:
    • Lei Jiang
    • Yi-Ming Zhao
    • Ming-Zhen Yang
  • View Affiliations / Copyright

    Affiliations: Department of Haematology, The Fourth Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230012, P.R. China
    Copyright: © Jiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 109
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    Published online on: December 11, 2020
       https://doi.org/10.3892/ol.2020.12370
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Abstract

Bortezomib is a novel proteasome inhibitor, which has been successfully used to treat mantle cell lymphoma and multiple myeloma. However, the direct effects of bortezomib on acute promyelocytic leukaemia (APL) have not been fully investigated. In the present study, the WST‑8 assay, western blotting, flow cytometry, monodansylcadaverine staining and transmission electron microscopy were performed. It was demonstrated that bortezomib treatment induced a time‑ and dose‑dependent decrease in the viability of NB4 cells. Bortezomib treatment induced cell apoptosis in NB4 cells, as assessed by Annexin V/propidium iodide analysis, and the detection of cleaved caspase‑3, cleaved poly(ADP‑ribose) polymerase, Bax and Bcl‑2 expression. Furthermore, bortezomib treatment induced autophagy in NB4 cells, as indicated by autophagosome formation, p62 degradation, LC3‑I to LC3‑II conversion and formation of acidic autophagic vacuoles. Notably, autophagy induced by bortezomib was initiated prior to apoptosis. Inhibition of autophagy by knocking down Beclin‑1 expression increased bortezomib‑induced apoptosis in the NB4 cells. Therefore, the present study revealed that the combination of bortezomib and autophagy inhibition may be a potential treatment strategy for APL.
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Copy and paste a formatted citation
Spandidos Publications style
Jiang L, Zhao Y and Yang M: Inhibition of autophagy enhances apoptosis induced by bortezomib in AML cells. Oncol Lett 21: 109, 2021.
APA
Jiang, L., Zhao, Y., & Yang, M. (2021). Inhibition of autophagy enhances apoptosis induced by bortezomib in AML cells. Oncology Letters, 21, 109. https://doi.org/10.3892/ol.2020.12370
MLA
Jiang, L., Zhao, Y., Yang, M."Inhibition of autophagy enhances apoptosis induced by bortezomib in AML cells". Oncology Letters 21.2 (2021): 109.
Chicago
Jiang, L., Zhao, Y., Yang, M."Inhibition of autophagy enhances apoptosis induced by bortezomib in AML cells". Oncology Letters 21, no. 2 (2021): 109. https://doi.org/10.3892/ol.2020.12370
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang L, Zhao Y and Yang M: Inhibition of autophagy enhances apoptosis induced by bortezomib in AML cells. Oncol Lett 21: 109, 2021.
APA
Jiang, L., Zhao, Y., & Yang, M. (2021). Inhibition of autophagy enhances apoptosis induced by bortezomib in AML cells. Oncology Letters, 21, 109. https://doi.org/10.3892/ol.2020.12370
MLA
Jiang, L., Zhao, Y., Yang, M."Inhibition of autophagy enhances apoptosis induced by bortezomib in AML cells". Oncology Letters 21.2 (2021): 109.
Chicago
Jiang, L., Zhao, Y., Yang, M."Inhibition of autophagy enhances apoptosis induced by bortezomib in AML cells". Oncology Letters 21, no. 2 (2021): 109. https://doi.org/10.3892/ol.2020.12370
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