Open Access

Dysregulation of phosphoproteins in hepatocellular carcinoma revealed via quantitative analysis of the phosphoproteome

  • Authors:
    • Yixian Liu
    • Qianwei Zhao
    • Fang Xu
    • Kaijuan Wang
    • Ying Zhao
    • Huiping Chen
    • Wei He
    • Weidong Wang
    • Jianying Zhang
    • Jintao Zhang
  • View Affiliations

  • Published online on: December 15, 2020     https://doi.org/10.3892/ol.2020.12378
  • Article Number: 117
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Hepatocellular carcinoma (HCC) is one of the most frequently diagnosed types of cancer in the world. Post‑translational modifications, such as phosphorylation, serve an essential role during cancer development. To identify aberrant phosphorylation in HCC, a multiplexed tandem mass tag approach combined with liquid chromatography tandem‑mass spectrometry was used in the present study. The results are available via ProteomeXchange (identifier no. PXD013934). A total of 4,780 phosphorylated sites distributed on 2,209 proteins were identified and quantified, including 74 and 459 phosphorylated upregulated and downregulated proteins, respectively. Bioinformatic analysis revealed differences and similarities between HCC and normal tissues. Gene Ontology enrichment analysis provided information on biological processes, molecular functions, cellular components and sub‑cellular localizations. Protein domains enrichment of differentially expressed proteins was analyzed using InterPro database. Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed pathways that may potentially be involved in HCC. Integrative analysis of the functions, pathways, motifs of phosphorylated peptides, protein domains and protein interactions established a profile of the phosphoproteome of HCC, which may contribute to identify novel biomarkers for the diagnosis and prognosis of HCC, as well as novel therapeutic targets for HCC treatment.
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February-2021
Volume 21 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Liu Y, Zhao Q, Xu F, Wang K, Zhao Y, Chen H, He W, Wang W, Zhang J, Zhang J, Zhang J, et al: Dysregulation of phosphoproteins in hepatocellular carcinoma revealed via quantitative analysis of the phosphoproteome. Oncol Lett 21: 117, 2021
APA
Liu, Y., Zhao, Q., Xu, F., Wang, K., Zhao, Y., Chen, H. ... Zhang, J. (2021). Dysregulation of phosphoproteins in hepatocellular carcinoma revealed via quantitative analysis of the phosphoproteome. Oncology Letters, 21, 117. https://doi.org/10.3892/ol.2020.12378
MLA
Liu, Y., Zhao, Q., Xu, F., Wang, K., Zhao, Y., Chen, H., He, W., Wang, W., Zhang, J., Zhang, J."Dysregulation of phosphoproteins in hepatocellular carcinoma revealed via quantitative analysis of the phosphoproteome". Oncology Letters 21.2 (2021): 117.
Chicago
Liu, Y., Zhao, Q., Xu, F., Wang, K., Zhao, Y., Chen, H., He, W., Wang, W., Zhang, J., Zhang, J."Dysregulation of phosphoproteins in hepatocellular carcinoma revealed via quantitative analysis of the phosphoproteome". Oncology Letters 21, no. 2 (2021): 117. https://doi.org/10.3892/ol.2020.12378