miR-21-5p promotes cell proliferation by targeting BCL11B in Thp-1 cells

  • Authors:
    • Liang Zhang
    • Li Yu
    • Yiran Liu
    • Shasha Wang
    • Zhenfeng Hou
    • Jun Zhou
  • View Affiliations

  • Published online on: December 15, 2020     https://doi.org/10.3892/ol.2020.12380
  • Article Number: 119
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Abstract

Acute myeloid leukemia (AML) is a highly heterogeneous disease that remains untreatable. MicroRNAs (miRNAs or miRs) play important roles in the pathogenesis of leukemia. miR‑21 is highly expressed in multiple types of human cancer and displays oncogenic activities; however, the clinical significance of miR‑21 in AML remains unclear. In the present study, it was demonstrated that miR‑21 levels were high in patients with AML and in AML cell lines. Further experiments demonstrated that overexpression of miR‑21 in Thp‑1 human monocytes derived from acute mononuclear leukemia peripheral blood promoted cell proliferation, while downregulation of miR‑21‑5p, a mature sequence derived from the 5' end of the miR‑21 stem‑loop precursor (another mature sequence, miR‑21‑3p, is derived form 3' end of miR‑21), inhibited cell proliferation. Specifically, it was observed that overexpression of miR‑21 could promote the transition of Thp‑1 cells into the S and G2/M phases of the cell cycle, as shown by flow cytometry. Furthermore, inhibition of miR‑21‑5p arrested cells in the S and G2/M phases. Finally, BCL11B was determined to be a functional target of miR‑21‑5p by luciferase assays. Our study revealed functional and mechanistic associations between miR‑21 and BCL11B in Thp‑1 cells, which could serve to guide clinical treatment of AML.
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February-2021
Volume 21 Issue 2

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Copy and paste a formatted citation
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Spandidos Publications style
Zhang L, Yu L, Liu Y, Wang S, Hou Z and Zhou J: miR-21-5p promotes cell proliferation by targeting BCL11B in Thp-1 cells. Oncol Lett 21: 119, 2021
APA
Zhang, L., Yu, L., Liu, Y., Wang, S., Hou, Z., & Zhou, J. (2021). miR-21-5p promotes cell proliferation by targeting BCL11B in Thp-1 cells. Oncology Letters, 21, 119. https://doi.org/10.3892/ol.2020.12380
MLA
Zhang, L., Yu, L., Liu, Y., Wang, S., Hou, Z., Zhou, J."miR-21-5p promotes cell proliferation by targeting BCL11B in Thp-1 cells". Oncology Letters 21.2 (2021): 119.
Chicago
Zhang, L., Yu, L., Liu, Y., Wang, S., Hou, Z., Zhou, J."miR-21-5p promotes cell proliferation by targeting BCL11B in Thp-1 cells". Oncology Letters 21, no. 2 (2021): 119. https://doi.org/10.3892/ol.2020.12380