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Fucosylated C4b‑binding protein α‑chain, a novel serum biomarker that predicts lymph node metastasis in pancreatic ductal adenocarcinoma

  • Authors:
    • Kazuyuki Sogawa
    • Sakino Yamanaka
    • Shigetsugu Takano
    • Kosuke Sasaki
    • Yoji Miyahara
    • Katsunori Furukawa
    • Tsukasa Takayashiki
    • Satoshi Kuboki
    • Hirotaka Takizawa
    • Fumio Nomura
    • Masayuki Ohtsuka
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry, School of Life and Environmental Science, Azabu University, Sagamihara, Kanagawa 252‑5201, Japan, Department of General Surgery, Graduate School of Medicine, Chiba University, Chiba 260‑8670, Japan, Kashiwado Clinic in Port‑Square, Kashiwado Memorial Foundation, Chiba 260‑0025, Japan, Divisions of Clinical Mass Spectrometry and Clinical Genetics, Chiba University Hospital, Chiba 260‑8670, Japan
    Copyright: © Sogawa et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 127
    |
    Published online on: December 17, 2020
       https://doi.org/10.3892/ol.2020.12388
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Abstract

C4b‑binding protein α‑chain (C4BPA) was previously identified as a novel serum biomarker for pancreatic ductal adenocarcinoma (PDAC). To apply this biomarker for clinical diagnosis, a lectin ELISA was established to measure serum fucosylated (Fuc)‑C4BPA levels in 45 patients with PDAC, 20 patients with chronic pancreatitis (CP) and 50 healthy volunteers (HVs) in one training and three validation sets. The lecithin ELISA developed in the current study exhibited satisfactory within‑run (2.6‑6.7%) and between‑day (1.8‑3.6%) coefficient of variations. Serum Fuc‑C4BPA levels in patients with PDAC (0.54±0.27 AU/ml) was significantly higher than that in HVs (0.21±0.06 AU/ml; P<0.0001) and patients with CP (0.25±0.03 AU/ml; P<0.0001). Additionally, serum Fuc‑C4BPA levels in preoperative patients were significantly decreased compared with postoperative patient sera (P<0.0003). The receiver operating characteristic (ROC) curve analyses revealed that the area under the curve (AUC) of Fuc‑C4BPA (0.985) was higher than that of carbohydrate antigen (CA)19‑9 (0.843), carcinoembryonic antigen (0.548) and total C4BPA (0.875) (P<0.001). To analyze the clinical significance of Fuc‑C4BPA, the ability of Fuc‑C4BPA to predict lymph node metastasis was compared with that of CA19‑9. The AUC of serum Fuc‑C4BPA levels (0.703) was significantly higher than that of serum CA19‑9 levels (0.500) in patients with PDAC (P<0.001). The current study established a novel lectin ELISA for measuring serum Fuc‑C4BPA levels. Thus, Fuc‑C4BPA has potential clinical applications owing to its high diagnostic value in PDAC.
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1 

Takano S, Yoshitomi H, Togawa A, Sogawa K, Shida T, Kimura F, Shimizu H, Tomonaga T, Nomura F and Miyazaki M: Apolipoprotein C-1 maintains cell survival by preventing from apoptosis in pancreatic cancer cells. Oncogene. 27:2810–2822. 2008. View Article : Google Scholar

2 

Moulder R, Bhosale SD, Goodlett DR and Lahesmaa R: Analysis of the plasma proteome using iTRAQ and TMT-based Isobaric labeling. Mass Spectrom Rev. 37:583–606. 2018. View Article : Google Scholar

3 

Bhawal R, Oberg AL, Zhang S and Kohli M: Challenges and opportunities in clinical applications of blood-based proteomics in cancer. Cancers (Basel). 12:24282020. View Article : Google Scholar

4 

Sogawa K, Takano S, Iida F, Satoh M, Tsuchida S, Kawashima Y, Yoshitomi H, Sanda A, Kodera Y, Takizawa H, et al: Identification of a novel serum biomarker for pancreatic cancer, C4b-binding protein α-chain (C4BPA) by quantitative proteomic analysis using tandem mass tags. Br J Cancer. 115:949–956. 2016. View Article : Google Scholar

5 

Mikami M, Tanabe K, Matsuo K, Miyazaki Y, Miyazawa M, Hayashi M, Asai S, Ikeda M, Shida M, Hirasawa T, et al: Fully-sialylated alpha-chain of complement 4-binding protein: Diagnostic utility for ovarian clear cell carcinoma. Gynecol Oncol. 139:520–528. 2015. View Article : Google Scholar

6 

Liu YS, Luo XY, Li QR, Li H, Li C, Ni H, Li RX, Wang R, Hu HC, Pan YJ, et al: Shotgun and targeted proteomics reveal that pre-surgery serum levels of LRG1, SAA, and C4BP may refine prognosis of resected squamous cell lung cancer. J Mol Cell Biol. 4:344–347. 2012. View Article : Google Scholar

7 

Jia L, Zhang J, Ma T, Guo Y, Yu Y and Cui J: The function of fucosylation in progression of lung cancer. Front Oncol. 8:5652018. View Article : Google Scholar

8 

Li D and Satomura S: Biomarkers for hepatocellular carcinoma (HCC): An update. Adv Exp Med Biol. 867:179–193. 2015. View Article : Google Scholar

9 

Jelski W and Mroczko B: Biochemical diagnostics of pancreatic cancer-Present and future. Clin Chim Acta. 498:47–51. 2019. View Article : Google Scholar

10 

Tanabe K, Matsuo K, Miyazawa M, Hayashi M, Ikeda M, Shida M, Hirasawa T, Sho R and Mikami M: UPLC-MS/MS based diagnostics for epithelial ovarian cancer using fully sialylated C4-binding protein. Biomed Chromatogr. 32:e41802018. View Article : Google Scholar

11 

Umemura H, Nezu M, Kodera Y, Satoh M, Kimura A, Tomonaga T and Nomura F: Effects of the time intervals between venipuncture and serum preparation for serum peptidome analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Clin Chim Acta. 406:179–180. 2009. View Article : Google Scholar

12 

Narimatsu H, Iwasaki H, Nakayama F, Ikehara Y, Kudo T, Nishihara S, Sugano K, Okura H, Fujita S and Hirohashi S: Lewis and secretor gene dosages affect CA19-9 and DU-PAN-2 serum levels in normal individuals and colorectal cancer patients. Cancer Res. 58:512–518. 1998.

13 

Kawa S, Oguchi H, Kobayashi T, Tokoo M, Furuta S, Kanai M and Homma T: Elevated serum levels of Dupan-2 in pancreatic cancer patients negative for Lewis blood group phenotype. Br J Cancer. 64:899–902. 1991. View Article : Google Scholar

14 

Stanczyk FZ and Clarke NJ: Advantages and challenges of mass spectrometry assays for steroid hormones. J Steroid Biochem Mol Biol. 121:491–495. 2010. View Article : Google Scholar

15 

Farrell CJ, Martin S, McWhinney B, Straub I, Williams P and Herrmann M: State-of-the-art vitamin D assays: A comparison of automated immunoassays with liquid chromatography-tandem mass spectrometry methods. Clin Chem. 58:531–542. 2012. View Article : Google Scholar

16 

Soldin SJ, Soukhova N, Janicic N, Jonklaas J and Soldin OP: The measurement of free thyroxine by isotope dilution tandem mass spectrometry. Clin Chim Acta. 358:113–118. 2005. View Article : Google Scholar

17 

Reis ES, Mastellos DC, Ricklin D, Mantovani A and Lambris JD: Complement in cancer: Untangling an intricate relationship. Nat Rev Immunol. 18:15–18. 2018. View Article : Google Scholar

18 

Muinelo-Romay L, Vazquez-Martin C, Villar-Portela S, Cuevas E, Gil-Martín E and Fernández-Briera A: Expression and enzyme activity of alpha (1,6) fucosyltransferase in human colorectal cancer. Int J Cancer. 123:641–646. 2008. View Article : Google Scholar

19 

Moriwaki K, Noda K, Furukawa Y, Ohshima K, Uchiyama A, Nakagawa T, Taniguchi N, Daigo Y, Nakamura Y, Hayashi N and Miyoshi E: Deficiency of GMDS leads to escape from NK cell-mediated tumor surveillance through modulation of TRAIL signaling. Gastroenterology. 137:188–198. 2009. View Article : Google Scholar

20 

Lambert A, Schwarz L, Borbath I, Henry A, Van Laethem JL, Malka D, Ducreux M and Conroy T: An update on treatment options for pancreatic adenocarcinoma. Ther Adv Med Oncol. 11:17588359198755682019. View Article : Google Scholar

21 

Tran Cao HS, Zhang Q, Sada YH, Silberfein EJ, Hsu C, Van Buren G II, Chai C, Katz MHG, Fisher WE and Massarweh NN: Value of lymph node positivity in treatment planning for early stage pancreatic cancer. Surgery. 162:557–567. 2017. View Article : Google Scholar

22 

Roche CJ, Hughes ML, Garvey CJ, Campbell F, White DA, Jones L and Neoptolemos JP: CT and pathologic assessment of prospective nodal staging in patients with ductal adenocarcinoma of the head of the pancreas. AJR Am J Roentgenol. 180:475–480. 2003. View Article : Google Scholar

23 

Tseng DS, van Santvoort HC, Fegrachi S, Besselink MG, Zuithoff NP, Borel Rinkes IH, van Leeuwen MS and Molenaar IQ: Diagnostic accuracy of CT in assessing extra-regional lymphadenopathy in pancreatic and peri-ampullary cancer: A systematic review and meta-analysis. Surg Oncol. 23:229–235. 2014. View Article : Google Scholar

24 

Nishiwada S, Sho M, Banwait JK, Yamamura K, Akahori T, Nakamura K, Baba H and Goel A: A microRNA signature identifies pancreatic ductal adenocarcinoma patients at risk for lymph node metastases. Gastroenterology. 159:562–574. 2020. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Sogawa K, Yamanaka S, Takano S, Sasaki K, Miyahara Y, Furukawa K, Takayashiki T, Kuboki S, Takizawa H, Nomura F, Nomura F, et al: Fucosylated C4b‑binding protein α‑chain, a novel serum biomarker that predicts lymph node metastasis in pancreatic ductal adenocarcinoma. Oncol Lett 21: 127, 2021.
APA
Sogawa, K., Yamanaka, S., Takano, S., Sasaki, K., Miyahara, Y., Furukawa, K. ... Ohtsuka, M. (2021). Fucosylated C4b‑binding protein α‑chain, a novel serum biomarker that predicts lymph node metastasis in pancreatic ductal adenocarcinoma. Oncology Letters, 21, 127. https://doi.org/10.3892/ol.2020.12388
MLA
Sogawa, K., Yamanaka, S., Takano, S., Sasaki, K., Miyahara, Y., Furukawa, K., Takayashiki, T., Kuboki, S., Takizawa, H., Nomura, F., Ohtsuka, M."Fucosylated C4b‑binding protein α‑chain, a novel serum biomarker that predicts lymph node metastasis in pancreatic ductal adenocarcinoma". Oncology Letters 21.2 (2021): 127.
Chicago
Sogawa, K., Yamanaka, S., Takano, S., Sasaki, K., Miyahara, Y., Furukawa, K., Takayashiki, T., Kuboki, S., Takizawa, H., Nomura, F., Ohtsuka, M."Fucosylated C4b‑binding protein α‑chain, a novel serum biomarker that predicts lymph node metastasis in pancreatic ductal adenocarcinoma". Oncology Letters 21, no. 2 (2021): 127. https://doi.org/10.3892/ol.2020.12388
Copy and paste a formatted citation
x
Spandidos Publications style
Sogawa K, Yamanaka S, Takano S, Sasaki K, Miyahara Y, Furukawa K, Takayashiki T, Kuboki S, Takizawa H, Nomura F, Nomura F, et al: Fucosylated C4b‑binding protein α‑chain, a novel serum biomarker that predicts lymph node metastasis in pancreatic ductal adenocarcinoma. Oncol Lett 21: 127, 2021.
APA
Sogawa, K., Yamanaka, S., Takano, S., Sasaki, K., Miyahara, Y., Furukawa, K. ... Ohtsuka, M. (2021). Fucosylated C4b‑binding protein α‑chain, a novel serum biomarker that predicts lymph node metastasis in pancreatic ductal adenocarcinoma. Oncology Letters, 21, 127. https://doi.org/10.3892/ol.2020.12388
MLA
Sogawa, K., Yamanaka, S., Takano, S., Sasaki, K., Miyahara, Y., Furukawa, K., Takayashiki, T., Kuboki, S., Takizawa, H., Nomura, F., Ohtsuka, M."Fucosylated C4b‑binding protein α‑chain, a novel serum biomarker that predicts lymph node metastasis in pancreatic ductal adenocarcinoma". Oncology Letters 21.2 (2021): 127.
Chicago
Sogawa, K., Yamanaka, S., Takano, S., Sasaki, K., Miyahara, Y., Furukawa, K., Takayashiki, T., Kuboki, S., Takizawa, H., Nomura, F., Ohtsuka, M."Fucosylated C4b‑binding protein α‑chain, a novel serum biomarker that predicts lymph node metastasis in pancreatic ductal adenocarcinoma". Oncology Letters 21, no. 2 (2021): 127. https://doi.org/10.3892/ol.2020.12388
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