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miR‑128‑3p serves as an oncogenic microRNA in osteosarcoma cells by downregulating ZC3H12D

  • Authors:
    • Maoshu Zhu
    • Yulong Wu
    • Zhaowei Wang
    • Minghua Lin
    • Bin Su
    • Chunyang Li
    • Fulong Liang
    • Xinjiang Chen
  • View Affiliations / Copyright

    Affiliations: Department of Central Laboratory, The Fifth Hospital of Xiamen, Xiang'an, Xiamen, Fujian 361000, P.R. China, Department of Urinary Surgery, The Fifth Hospital of Xiamen, Xiang'an, Xiamen, Fujian 361000, P.R. China, Department of Gynecology, The Fifth Hospital of Xiamen, Xiang'an, Xiamen, Fujian 361000, P.R. China, Department of Pathology, The Fifth Hospital of Xiamen, Xiang'an, Xiamen, Fujian 361000, P.R. China, Department of Pharmacy, The Fifth Hospital of Xiamen, Xiang'an, Xiamen, Fujian 361000, P.R. China, Department of Orthopedics, The Fifth Hospital of Xiamen, Xiang'an, Xiamen, Fujian 361000, P.R. China, Department of Neurology, The Fifth Hospital of Xiamen, Xiang'an, Xiamen, Fujian 361000, P.R. China
    Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 152
    |
    Published online on: December 31, 2020
       https://doi.org/10.3892/ol.2020.12413
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Abstract

Osteosarcoma is the second leading cause of cancer‑associated mortality worldwide in children and adolescents. ZC3H12D has been shown to negatively regulate Toll‑like receptor signaling and serves as a possible tumor suppressor gene. MicroRNAs (miRNAs/miRs) are known to play an important role in the proliferation of human osteosarcoma cells. However, whether miRNAs can affect tumor development by regulating the expression of ZC3H12D has not yet been investigated. The aim of the present study was to investigate the role of miR128‑3p in regulating ZC3H12D expression, as well as its function in tumor cell proliferation, apoptosis, and metastasis. Reverse transcription‑quantitative PCR, western blotting and dual luciferase reporter assays were performed to analyze the regulation of ZC3H12D expression by miR‑128‑3p. MTT, colony formation and flow cytometry assays were also used to analyze the effect of miR‑128‑3p on cell proliferation and apoptosis. A wound healing assay was performed to investigate the cell migration ability. The results demonstrated that miR‑128‑3p directly targeted ZC3H12D and downregulated its expression, thereby promoting cell proliferation and migration. miR‑128‑3p overexpression also improved resistance to cisplatin in MG‑63 and 143B cell lines, supporting the hypothesis that miR‑128‑3p may function as an oncogene in osteosarcoma cells. The potential clinical significance of miR‑128‑3p as a biomarker and therapeutic target provides rationale for further investigation into the miR‑128‑3p‑mediated molecular pathway and how it is associated with osteosarcoma development.
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Copy and paste a formatted citation
Spandidos Publications style
Zhu M, Wu Y, Wang Z, Lin M, Su B, Li C, Liang F and Chen X: miR‑128‑3p serves as an oncogenic microRNA in osteosarcoma cells by downregulating ZC3H12D. Oncol Lett 21: 152, 2021.
APA
Zhu, M., Wu, Y., Wang, Z., Lin, M., Su, B., Li, C. ... Chen, X. (2021). miR‑128‑3p serves as an oncogenic microRNA in osteosarcoma cells by downregulating ZC3H12D. Oncology Letters, 21, 152. https://doi.org/10.3892/ol.2020.12413
MLA
Zhu, M., Wu, Y., Wang, Z., Lin, M., Su, B., Li, C., Liang, F., Chen, X."miR‑128‑3p serves as an oncogenic microRNA in osteosarcoma cells by downregulating ZC3H12D". Oncology Letters 21.2 (2021): 152.
Chicago
Zhu, M., Wu, Y., Wang, Z., Lin, M., Su, B., Li, C., Liang, F., Chen, X."miR‑128‑3p serves as an oncogenic microRNA in osteosarcoma cells by downregulating ZC3H12D". Oncology Letters 21, no. 2 (2021): 152. https://doi.org/10.3892/ol.2020.12413
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu M, Wu Y, Wang Z, Lin M, Su B, Li C, Liang F and Chen X: miR‑128‑3p serves as an oncogenic microRNA in osteosarcoma cells by downregulating ZC3H12D. Oncol Lett 21: 152, 2021.
APA
Zhu, M., Wu, Y., Wang, Z., Lin, M., Su, B., Li, C. ... Chen, X. (2021). miR‑128‑3p serves as an oncogenic microRNA in osteosarcoma cells by downregulating ZC3H12D. Oncology Letters, 21, 152. https://doi.org/10.3892/ol.2020.12413
MLA
Zhu, M., Wu, Y., Wang, Z., Lin, M., Su, B., Li, C., Liang, F., Chen, X."miR‑128‑3p serves as an oncogenic microRNA in osteosarcoma cells by downregulating ZC3H12D". Oncology Letters 21.2 (2021): 152.
Chicago
Zhu, M., Wu, Y., Wang, Z., Lin, M., Su, B., Li, C., Liang, F., Chen, X."miR‑128‑3p serves as an oncogenic microRNA in osteosarcoma cells by downregulating ZC3H12D". Oncology Letters 21, no. 2 (2021): 152. https://doi.org/10.3892/ol.2020.12413
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