Open Access

MicroRNA‑640 promotes cell proliferation and adhesion in glioblastoma by targeting Slit guidance ligand 1

  • Authors:
    • Chao Luo
    • Zhiying Lu
    • Yongli Chen
    • Xiaozhen Chen
    • Na Liu
    • Jing Chen
    • Shanwu Dong
  • View Affiliations

  • Published online on: December 31, 2020     https://doi.org/10.3892/ol.2020.12422
  • Article Number: 161
  • Copyright: © Luo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The effects of microRNAs (miRNAs/miRs) on glioblastoma have attracted the attention of researchers in the last 7 years. However, the role of miR‑640 and its targeted gene, Slit guidance ligand 1 (SLIT1), in the development of glioblastoma are not yet fully understood. The present study aimed to investigate the role of miR‑640 in the proliferation and adhesion of glioblastoma. Reverse transcription‑quantitative PCR analysis was performed to detect miR‑640 and SLIT1 expression in glioblastoma tissues and cells. In addition, the Dual‑luciferase reporter and RNA‑pull down assays were performed to assess the association between miR‑640 and SLIT1. The Cell Counting Kit‑8, BrdU ELISA, cell adhesion and caspase‑3 activity assays were also performed to assess cell viability, proliferation, adhesion and apoptosis of glioblastoma cells, respectively. The results demonstrated that miR‑640 expression was upregulated in glioblastoma tissues and cells. In addition, miR‑640 promoted the cell viability, proliferation and adhesion of glioblastoma cells, while inhibiting cell apoptosis. SLIT1, a direct downstream target of miR‑640, was demonstrated to be downregulated in glioblastoma tissues and cells. Furthermore, overexpression of SLIT1 attenuated the promotive effect of miR‑640 on glioblastoma cells. Taken together, these results suggest that miR‑640 accelerates the proliferation and adhesion of glioblastoma cell lines by targeting and suppressing SLIT1.
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February-2021
Volume 21 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Luo C, Lu Z, Chen Y, Chen X, Liu N, Chen J and Dong S: MicroRNA‑640 promotes cell proliferation and adhesion in glioblastoma by targeting Slit guidance ligand 1. Oncol Lett 21: 161, 2021
APA
Luo, C., Lu, Z., Chen, Y., Chen, X., Liu, N., Chen, J., & Dong, S. (2021). MicroRNA‑640 promotes cell proliferation and adhesion in glioblastoma by targeting Slit guidance ligand 1. Oncology Letters, 21, 161. https://doi.org/10.3892/ol.2020.12422
MLA
Luo, C., Lu, Z., Chen, Y., Chen, X., Liu, N., Chen, J., Dong, S."MicroRNA‑640 promotes cell proliferation and adhesion in glioblastoma by targeting Slit guidance ligand 1". Oncology Letters 21.2 (2021): 161.
Chicago
Luo, C., Lu, Z., Chen, Y., Chen, X., Liu, N., Chen, J., Dong, S."MicroRNA‑640 promotes cell proliferation and adhesion in glioblastoma by targeting Slit guidance ligand 1". Oncology Letters 21, no. 2 (2021): 161. https://doi.org/10.3892/ol.2020.12422