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Identification of prognostic biomarkers associated with metastasis and immune infiltration in osteosarcoma

  • Authors:
    • Bingsheng Yang
    • Zexin Su
    • Guoli Chen
    • Zhirui Zeng
    • Jianye Tan
    • Guofeng Wu
    • Shuang Zhu
    • Lijun Lin
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedics, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong 510282, P.R. China, Department of Joint Surgery, Huadu District People's Hospital, Southern Medical University, Guangzhou, Guangdong 510800, P.R. China, Department of Orthopaedics, Affiliated Hospital of Putian University, Putian, Fujian 351100, P.R. China, Guizhou Provincial Key Laboratory of Pathogenesis and Drug Research on Common Chronic Diseases, Department of Physiology, School of Basic Medicine, Guizhou Medical University, Guiyang, Guizhou 550009, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 180
    |
    Published online on: January 6, 2021
       https://doi.org/10.3892/ol.2021.12441
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Abstract

Osteosarcoma is the most common primary malignancy of the bones, and is associated with a high rate of metastasis and a poor prognosis. A tight association between the tumor microenvironment (TME) and osteosarcoma metastasis has been established. In the present study, the Estimation of STromal and Immune cells in MAlignant Tumor tissues using Expression data (ESTIMATE) algorithm was applied to calculate the immune and stromal scores of patients with osteosarcoma based on data from The Cancer Genome Atlas database. A metagene approach and deconvolution method were used to reveal distinct TME landscapes in patients with osteosarcoma. Bioinformatics analysis was used to identify differentially expressed genes (DEGs) associated with metastasis and immune infiltration in osteosarcoma, and a risk model was constructed using the DEGs with potential prognostic significance. Subsequently, gene set enrichment and Spearman's correlation analyses were used to delineate the biological processes associated with these prognostic biomarkers. Finally, immunohistochemical (IHC) analysis was performed to evaluate the expression levels of immune infiltrates and prognostic biomarkers in clinical osteosarcoma tissues. The results of the ESTIMATE demonstrated that patients with non‑metastatic osteosarcoma presented with higher immune/stromal scores and a more favorable prognosis compared with those with metastatic osteosarcoma. The TME landscapes in patients with osteosarcoma suggested that high levels of tumor‑infiltrating immune cells (TIICs) may suppress metastasis. Increased numbers of CD56bright natural killer cells, immature B cells, M1 macrophages and neutrophils, and lower levels of M2 macrophages were observed in the non‑metastatic tissues compared with those in the metastatic tissues. A total of 69 DEGs were identified to be associated with metastasis and immune infiltration in osteosarcoma. Of these, GATA3, LPAR5, EVI2B, RIAM and CFH exhibited prognostic potential and were highly expressed in non‑metastatic osteosarcoma tissues based on the IHC analysis results. These biomarkers were involved in various immune‑related biological processes and were positively associated with multiple TIICs and immune signatures. The risk model constructed using these prognostic biomarkers demonstrated high predictive accuracy for the prognosis of osteosarcoma. In conclusion, the present study proposed a five‑biomarker prognostic signature for the prediction of metastasis and immune infiltration in patients with osteosarcoma.
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Copy and paste a formatted citation
Spandidos Publications style
Yang B, Su Z, Chen G, Zeng Z, Tan J, Wu G, Zhu S and Lin L: Identification of prognostic biomarkers associated with metastasis and immune infiltration in osteosarcoma. Oncol Lett 21: 180, 2021.
APA
Yang, B., Su, Z., Chen, G., Zeng, Z., Tan, J., Wu, G. ... Lin, L. (2021). Identification of prognostic biomarkers associated with metastasis and immune infiltration in osteosarcoma. Oncology Letters, 21, 180. https://doi.org/10.3892/ol.2021.12441
MLA
Yang, B., Su, Z., Chen, G., Zeng, Z., Tan, J., Wu, G., Zhu, S., Lin, L."Identification of prognostic biomarkers associated with metastasis and immune infiltration in osteosarcoma". Oncology Letters 21.3 (2021): 180.
Chicago
Yang, B., Su, Z., Chen, G., Zeng, Z., Tan, J., Wu, G., Zhu, S., Lin, L."Identification of prognostic biomarkers associated with metastasis and immune infiltration in osteosarcoma". Oncology Letters 21, no. 3 (2021): 180. https://doi.org/10.3892/ol.2021.12441
Copy and paste a formatted citation
x
Spandidos Publications style
Yang B, Su Z, Chen G, Zeng Z, Tan J, Wu G, Zhu S and Lin L: Identification of prognostic biomarkers associated with metastasis and immune infiltration in osteosarcoma. Oncol Lett 21: 180, 2021.
APA
Yang, B., Su, Z., Chen, G., Zeng, Z., Tan, J., Wu, G. ... Lin, L. (2021). Identification of prognostic biomarkers associated with metastasis and immune infiltration in osteosarcoma. Oncology Letters, 21, 180. https://doi.org/10.3892/ol.2021.12441
MLA
Yang, B., Su, Z., Chen, G., Zeng, Z., Tan, J., Wu, G., Zhu, S., Lin, L."Identification of prognostic biomarkers associated with metastasis and immune infiltration in osteosarcoma". Oncology Letters 21.3 (2021): 180.
Chicago
Yang, B., Su, Z., Chen, G., Zeng, Z., Tan, J., Wu, G., Zhu, S., Lin, L."Identification of prognostic biomarkers associated with metastasis and immune infiltration in osteosarcoma". Oncology Letters 21, no. 3 (2021): 180. https://doi.org/10.3892/ol.2021.12441
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