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A comprehensive analysis of the expression, epigenetic and genetic changes of HNF1B and ECI2 in 122 cases of high‑grade serous ovarian carcinoma

  • Authors:
    • Kristýna Němejcová
    • Michaela Bártů
    • Jan Hojný
    • Nikola Hájková
    • Romana Michálková
    • Eva Krkavcová
    • Ivana Stružinská
    • Hiep Quang Bui
    • Pavel Dundr
    • David Cibula
    • Kateřina Jirsová
  • View Affiliations / Copyright

    Affiliations: Institute of Pathology, First Faculty of Medicine, Charles University and General University Hospital in Prague, 12800 Prague, Czech Republic, Gynecologic Oncology Center, Department of Obstetrics and Gynecology, First Faculty of Medicine, Charles University and General University Hospital in Prague, 12800 Prague, Czech Republic, Institute of Biology and Medical Genetics, First Faculty of Medicine, Charles University and General University Hospital in Prague, 12800 Prague, Czech Republic
    Copyright: © Němejcová et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 185
    |
    Published online on: January 6, 2021
       https://doi.org/10.3892/ol.2021.12446
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Abstract

High‑grade serous ovarian cancer (HGSC) is the most common subtype of ovarian cancer, with a poor prognosis; however, most studies concerning ovarian carcinoma have focused mainly on clear cell carcinoma. The involvement of hepatocyte nuclear factor 1β (HNF1B) in the carcinogenesis of HGSC has not yet been fully elucidated. To the best of our knowledge, the present study is the first to analyse the expression of the possible downstream target of HNF1B, enoyl‑CoA (Δ) isomerase 2 (ECI2), in HGSC. The present study performed a comprehensive analysis of HNF1B mRNA and protein expression, and epigenetic and genetic changes, as well as an analysis of ECI2 mRNA and protein expression in 122 cases of HGSC. HNF1B protein expression was detected in 28/122 cases, and was positively associated with lymphovascular invasion (P=0.025). Protein expression of ECI2 was detected in 115/122 cases, but no associations with clinicopathological variables were revealed. Therefore, ECI2 does not seem to function as a suitable prognostic marker for HGSC. In the sample set, a positive correlation between HNF1B and ECI2 protein expression was detected (P=0.005). HNF1B mRNA was also positively correlated with HNF1B protein expression (P=0.001). HNF1B promoter methylation was detected in 26/67 (38.8%) of cases. A novel pathogenic somatic HNF1B mutation was detected in 1/61 (1.6%) of the analysed HGSC cases. No other correlations between the examined SNPs (rs4430796, rs757210 and rs7405776), HNF1B promoter methylation, HNF1B/ECI2 expression or clinicopathological characteristics were found.
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Copy and paste a formatted citation
Spandidos Publications style
Němejcová K, Bártů M, Hojný J, Hájková N, Michálková R, Krkavcová E, Stružinská I, Bui HQ, Dundr P, Cibula D, Cibula D, et al: A comprehensive analysis of the expression, epigenetic and genetic changes of HNF1B and ECI2 in 122 cases of high‑grade serous ovarian carcinoma. Oncol Lett 21: 185, 2021.
APA
Němejcová, K., Bártů, M., Hojný, J., Hájková, N., Michálková, R., Krkavcová, E. ... Jirsová, K. (2021). A comprehensive analysis of the expression, epigenetic and genetic changes of HNF1B and ECI2 in 122 cases of high‑grade serous ovarian carcinoma. Oncology Letters, 21, 185. https://doi.org/10.3892/ol.2021.12446
MLA
Němejcová, K., Bártů, M., Hojný, J., Hájková, N., Michálková, R., Krkavcová, E., Stružinská, I., Bui, H. Q., Dundr, P., Cibula, D., Jirsová, K."A comprehensive analysis of the expression, epigenetic and genetic changes of HNF1B and ECI2 in 122 cases of high‑grade serous ovarian carcinoma". Oncology Letters 21.3 (2021): 185.
Chicago
Němejcová, K., Bártů, M., Hojný, J., Hájková, N., Michálková, R., Krkavcová, E., Stružinská, I., Bui, H. Q., Dundr, P., Cibula, D., Jirsová, K."A comprehensive analysis of the expression, epigenetic and genetic changes of HNF1B and ECI2 in 122 cases of high‑grade serous ovarian carcinoma". Oncology Letters 21, no. 3 (2021): 185. https://doi.org/10.3892/ol.2021.12446
Copy and paste a formatted citation
x
Spandidos Publications style
Němejcová K, Bártů M, Hojný J, Hájková N, Michálková R, Krkavcová E, Stružinská I, Bui HQ, Dundr P, Cibula D, Cibula D, et al: A comprehensive analysis of the expression, epigenetic and genetic changes of HNF1B and ECI2 in 122 cases of high‑grade serous ovarian carcinoma. Oncol Lett 21: 185, 2021.
APA
Němejcová, K., Bártů, M., Hojný, J., Hájková, N., Michálková, R., Krkavcová, E. ... Jirsová, K. (2021). A comprehensive analysis of the expression, epigenetic and genetic changes of HNF1B and ECI2 in 122 cases of high‑grade serous ovarian carcinoma. Oncology Letters, 21, 185. https://doi.org/10.3892/ol.2021.12446
MLA
Němejcová, K., Bártů, M., Hojný, J., Hájková, N., Michálková, R., Krkavcová, E., Stružinská, I., Bui, H. Q., Dundr, P., Cibula, D., Jirsová, K."A comprehensive analysis of the expression, epigenetic and genetic changes of HNF1B and ECI2 in 122 cases of high‑grade serous ovarian carcinoma". Oncology Letters 21.3 (2021): 185.
Chicago
Němejcová, K., Bártů, M., Hojný, J., Hájková, N., Michálková, R., Krkavcová, E., Stružinská, I., Bui, H. Q., Dundr, P., Cibula, D., Jirsová, K."A comprehensive analysis of the expression, epigenetic and genetic changes of HNF1B and ECI2 in 122 cases of high‑grade serous ovarian carcinoma". Oncology Letters 21, no. 3 (2021): 185. https://doi.org/10.3892/ol.2021.12446
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