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Key genes in youth colorectal cancer based on data mining and verification by reverse transcription‑quantitative PCR

  • Authors:
    • Jianxin Chen
    • Min Lin
    • Yan Che
    • Jian Guo
    • Wei Lin
  • View Affiliations / Copyright

    Affiliations: The First Department of Gastrointestinal Surgery, The Affiliated Hospital of Putian University, Putian, Fujian 351100, P.R. China, College of Information Engineering, Putian University, Putian, Fujian 351100, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 194
    |
    Published online on: January 8, 2021
       https://doi.org/10.3892/ol.2021.12455
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Abstract

In recent years, among all patients with colorectal cancer, the proportion of young patients has been gradually increasing. However, the molecular mechanisms involved in colorectal cancer in the young are largely unknown. In the present study the Gene Expression Omnibus (GEO) and The Cancer Genome Atlas datasets were integrated to elucidate the key gene biomarkers in these patients. The GSE41657 and GSE41258 datasets were downloaded from the GEO database. By screening for differentially expressed genes, Gene Ontology analysis, Kyoto Encyclopedia of Genes and Genomes pathway analysis, protein‑protein interaction analysis, hub gene screening and survival analysis, two key genes, CXCL8 and VEGFA, which were enriched in cancer pathways, were obtained. Reverse transcription‑quantitative (RT‑q)PCR was performed to verify the outcome obtained by bioinformatics analysis. In conclusion, the present study identified two key genes using bioinformatics analysis and RT‑qPCR validation. These results indicated that the candidate genes may be involved in the progression of colorectal cancer in young people, and these two genes may act as ideal prognostic indicators or therapeutic targets for colorectal cancer in the youth.
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1 

Naeem A, Tun AM and Guevara E: Molecular genetics and the role of molecularly targeted agents in metastatic colorectal carcinoma. J Gastrointest Cancer. 51:387–400. 2020. View Article : Google Scholar : PubMed/NCBI

2 

Weinberg BA, Marshall JL and Salem ME: The growing challenge of young adults with colorectal cancer. Oncology (Williston Park). 31:381–389. 2017.PubMed/NCBI

3 

Arnold M, Sierra MS, Laversanne M, Soerjomataram I, Jemal A and Bray F: Global patterns and trends in colorectal cancer incidence and mortality. Gut. 66:683–691. 2017. View Article : Google Scholar : PubMed/NCBI

4 

Ravegnini G and Angelini S: Toward precision medicine: How far is the goal? Int J Mol Sci. 17:2452016. View Article : Google Scholar : PubMed/NCBI

5 

Johnson TM: Perspective on precision medicine in oncology. Pharmacotherapy. 37:988–989. 2017. View Article : Google Scholar : PubMed/NCBI

6 

Wei Q: Personalized medicine at a prime time for cancer medicine-introducing cancer medicine. Cancer Med. 1:12012. View Article : Google Scholar : PubMed/NCBI

7 

Mork ME, You YN, Ying J, Bannon SA, Lynch PM, Rodriguez-Bigas MA and Vilar E: High prevalence of hereditary cancer syndromes in adolescents and young adults with colorectal cancer. J Clin Oncol. 33:3544–3549. 2015. View Article : Google Scholar : PubMed/NCBI

8 

Khan SA, Morris M, Idrees K, Gimbel MI, Rosenberg S, Zeng Z, Li F, Gan G, Shia J, LaQuaglia MP and Paty PB: Colorectal cancer in the very young: A comparative study of tumor markers, pathology and survival in early onset and adult onset patients. J Pediatr Surg. 51:1812–1817. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Magnani G, Furlan D, Sahnane N, Reggiani Bonetti L, Domati F and Pedroni M: Molecular features and methylation status in early onset (≤40 years) colorectal cancer: A population based, case-control study. Gastroenterol Res Pract. 2015:1321902015. View Article : Google Scholar : PubMed/NCBI

10 

Pearlman R, Frankel WL, Swanson B, Zhao W, Yilmaz A, Miller K, Bacher J, Bigley C, Nelsen L, Goodfellow PJ, et al: Prevalence and spectrum of germline cancer susceptibility gene mutations among patients with early-onset colorectal cancer. JAMA Oncol. 3:464–471. 2017. View Article : Google Scholar : PubMed/NCBI

11 

Barrett T, Wilhite SE, Ledoux P, Evangelista C, Kim IF, Tomashevsky M, Marshall KA, Phillippy KH, Sherman PM, Holko M, et al: NCBI GEO: Archive for functional genomics data sets-update. Nucleic Acids Res. 41((Database Issue)): D991–D995. 2013.PubMed/NCBI

12 

Tang Z, Li C, Kang B, Gao G, Li C and Zhang Z: GEPIA: A web server for cancer and normal gene expression profiling and interactive analyses. Nucleic Acids Res. 45((W1)): W98–W102. 2017. View Article : Google Scholar : PubMed/NCBI

13 

Uhlén M, Fagerberg L, Hallström BM, Lindskog C, Oksvold P, Mardinoglu A, Sivertsson Å, Kampf C, Sjöstedt E, Asplund A, et al: Proteomics. Tissue-based map of the human proteome. Science. 347:12604192015. View Article : Google Scholar : PubMed/NCBI

14 

Schmittgen TD and Livak KJ: Analyzing real-time PCR data by the comparative C(T) method. Nat Protoc. 3:1101–1108. 2008. View Article : Google Scholar : PubMed/NCBI

15 

Yang L, Wang J, Sun X, Cao Y, Ning S, Zhang H, Chen L, Li R, Tian Q, Wang L, et al: Identifying a polymorphic ‘switch’ that influences miRNAs' regulation of a myasthenia gravis risk pathway. PLoS One. 9:e1048272014. View Article : Google Scholar : PubMed/NCBI

16 

Zhou X, Zheng R, Zhang H and He T: Pathway crosstalk analysis of microarray gene expression profile in human hepatocellular carcinoma. Pathol oncol Res. 21:563–569. 2015. View Article : Google Scholar : PubMed/NCBI

17 

Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA and Jemal A: Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 68:394–424. 2018. View Article : Google Scholar : PubMed/NCBI

18 

Chen W, Zheng R, Baade PD, Zhang S, Zeng H, Bray F, Jemal A, Yu XQ and He J: Cancer statistics in China, 2015. CA Cancer J Clin. 66:115–132. 2016. View Article : Google Scholar : PubMed/NCBI

19 

Marjon PL, Bobrovnikova-Marjon EV and Abcouwer SF: Expression of the pro-angiogenic factors vascular endothelial growth factor and interleukin-8/CXCL8 by human breast carcinomas is responsive to nutrient deprivation and endoplasmic reticulum stress. Mol Cancer. 3:42004. View Article : Google Scholar : PubMed/NCBI

20 

Matsushima K, Baldwin ET and Mukaida N: Interleukin-8 and MCAF: Novel leukocyte recruitment and activating cytokines. Chem Immunol. 51:236–265. 1992. View Article : Google Scholar : PubMed/NCBI

21 

Liu Q, Li A, Tian Y, Wu JD, Liu Y, Li T, Chen Y, Han X and Wu K: The CXCL8-CXCR1/2 pathways in cancer. Cytokine Growth Factor Rev. 31:61–71. 2016. View Article : Google Scholar : PubMed/NCBI

22 

Masuya D, Huang C, Liu D, Kameyama K, Hayashi E, Yamauchi A, Kobayashi S, Haba R and Yokomise H: The intratumoral expression of vascular endothelial growth factor and interleukin-8 associated with angiogenesis in nonsmall cell lung carcinoma patients. Cancer. 92:2628–2638. 2001. View Article : Google Scholar : PubMed/NCBI

23 

Yamamoto Y, Kuroda K, Sera T, Sugimoto A, Kushiyama S, Nishimura S, Togano S, Okuno T, Yoshii M, Tamura T, et al: The clinicopathological significance of the CXCR2 ligands, CXCL1, CXCL2, CXCL3, CXCL5, CXCL6, CXCL7, and CXCL8 in gastric cancer. Anticancer Res. 39:6645–6652. 2019. View Article : Google Scholar : PubMed/NCBI

24 

Liubomirski Y, Lerrer S, Meshel T, Morein D, Rubinstein-Achiasaf L, Sprinzak D, Wiemann S, Körner C, Ehrlich M and Ben-Baruch A: Notch-mediated tumor-stroma-inflammation networks promote invasive properties and CXCL8 expression in triple-negative breast cancer. Front Immunol. 10:8042019. View Article : Google Scholar : PubMed/NCBI

25 

Shen T, Yang Z, Cheng X, Xiao Y, Yu K, Cai X, Xia C and Li Y: CXCL8 induces epithelial-mesenchymal transition in colon cancer cells via the PI3K/Akt/NF-κB signaling pathway. Oncol Rep. 37:2095–2100. 2017. View Article : Google Scholar : PubMed/NCBI

26 

Doll D, Keller L, Maak M, Boulesteix AL, Siewert JR, Holzmann B and Janssen KP: Differential expression of the chemokines GRO-2, GRO-3, and interleukin-8 in colon cancer and their impact on metastatic disease and survival. Int J Colorectal Dis. 25:573–581. 2010. View Article : Google Scholar : PubMed/NCBI

27 

Zhao QQ, Jiang C, Gao Q, Zhang YY, Wang G, Chen XP, Wu SB and Tang J: Gene expression and methylation profiles identified CXCL3 and CXCL8 as key genes for diagnosis and prognosis of colon adenocarcinoma. J Cell Physiol. 235:4902–4912. 2020. View Article : Google Scholar : PubMed/NCBI

28 

Das ST, Rajagopalan L, Guerrero-Plata A, Sai J, Richmond A, Garofalo RP and Rajarathnam K: Monomeric and dimeric CXCL8 are both essential for in vivo neutrophil recruitment. PLoS One. 5:e117542010. View Article : Google Scholar : PubMed/NCBI

29 

Ferrara N: VEGF and the quest for tumour angiogenesis factors. Nat Rev Cancer. 2:795–803. 2002. View Article : Google Scholar : PubMed/NCBI

30 

Herbert SP and Stainier DY: Molecular control of endothelial cell behaviour during blood vessel morphogenesis. Nat Rev Mol Cell Biol. 12:551–564. 2011. View Article : Google Scholar : PubMed/NCBI

31 

Zhang SD, McCrudden CM and Kwok HF: Prognostic significance of combining VEGFA, FLT1 and KDR mRNA expression in lung cancer. Oncol Lett. 10:1893–1901. 2015. View Article : Google Scholar : PubMed/NCBI

32 

Lu J, Wang YH, Yoon C, Huang XY, Xu Y, Xie JW, Wang JB, Lin JX, Chen QY, Cao LL, et al: Circular RNA circ-RanGAP1 regulates VEGFA expression by targeting miR-877-3p to facilitate gastric cancer invasion and metastasis. Cancer Lett. 471:38–48. 2020. View Article : Google Scholar : PubMed/NCBI

33 

Korobeinikova E, Ugenskiene R, Insodaite R, Rudzianskas V, Jaselske E, Poskiene L and Juozaityte E: Association of angiogenesis and inflammation-related gene functional polymorphisms with early-stage breast cancer prognosis. Oncol Lett. 19:3687–3700. 2020.PubMed/NCBI

34 

Zhou P, Xiong T, Chen J, Li F, Qi T and Yuan J: Clinical significance of melanoma cell adhesion molecule CD146 and VEGFA expression in epithelial ovarian cancer. Oncol Lett. 17:2418–2424. 2019.PubMed/NCBI

35 

Wang R, Ma Y, Zhan S, Zhang G, Cao L, Zhang X, Shi T and Chen W: B7-H3 promotes colorectal cancer angiogenesis through activating the NF-κB pathway to induce VEGFA expression. Cell Death Dis. 11:552020. View Article : Google Scholar : PubMed/NCBI

36 

Ferrara N and Adamis AP: Ten years of anti-vascular endothelial growth factor therapy. Nat Rev Drug Discov. 15:385–403. 2016. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Chen J, Lin M, Che Y, Guo J and Lin W: Key genes in youth colorectal cancer based on data mining and verification by reverse transcription‑quantitative PCR. Oncol Lett 21: 194, 2021.
APA
Chen, J., Lin, M., Che, Y., Guo, J., & Lin, W. (2021). Key genes in youth colorectal cancer based on data mining and verification by reverse transcription‑quantitative PCR. Oncology Letters, 21, 194. https://doi.org/10.3892/ol.2021.12455
MLA
Chen, J., Lin, M., Che, Y., Guo, J., Lin, W."Key genes in youth colorectal cancer based on data mining and verification by reverse transcription‑quantitative PCR". Oncology Letters 21.3 (2021): 194.
Chicago
Chen, J., Lin, M., Che, Y., Guo, J., Lin, W."Key genes in youth colorectal cancer based on data mining and verification by reverse transcription‑quantitative PCR". Oncology Letters 21, no. 3 (2021): 194. https://doi.org/10.3892/ol.2021.12455
Copy and paste a formatted citation
x
Spandidos Publications style
Chen J, Lin M, Che Y, Guo J and Lin W: Key genes in youth colorectal cancer based on data mining and verification by reverse transcription‑quantitative PCR. Oncol Lett 21: 194, 2021.
APA
Chen, J., Lin, M., Che, Y., Guo, J., & Lin, W. (2021). Key genes in youth colorectal cancer based on data mining and verification by reverse transcription‑quantitative PCR. Oncology Letters, 21, 194. https://doi.org/10.3892/ol.2021.12455
MLA
Chen, J., Lin, M., Che, Y., Guo, J., Lin, W."Key genes in youth colorectal cancer based on data mining and verification by reverse transcription‑quantitative PCR". Oncology Letters 21.3 (2021): 194.
Chicago
Chen, J., Lin, M., Che, Y., Guo, J., Lin, W."Key genes in youth colorectal cancer based on data mining and verification by reverse transcription‑quantitative PCR". Oncology Letters 21, no. 3 (2021): 194. https://doi.org/10.3892/ol.2021.12455
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