MicroRNA‑182 promotes epithelial‑mesenchymal transition by targeting FOXN3 in gallbladder cancer

Zhang,Jianhong; Hu,Zeming; Wen,Chao; Liao,Qicheng; He,Baoqing; Peng,Jing; Tang,Xin; Chen,Zhixi; Xie,Yuankang

doi:10.3892/ol.2021.12461

MicroRNA‑182 promotes epithelial‑mesenchymal transition by targeting FOXN3 in gallbladder cancer

Authors:
- Jianhong Zhang
- Zeming Hu
- Chao Wen
- Qicheng Liao
- Baoqing He
- Jing Peng
- Xin Tang
- Zhixi Chen
- Yuankang Xie
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Affiliations: Department of Hepatobiliary Surgery, The First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi 341000, P.R. China, Department of General Surgery, Zhejiang Xiaoshan Hospital, Hangzhou, Zhejiang 311202, P.R. China, School of Nursing, Gannan Medical University, Ganzhou, Jiangxi 341000, P.R. China, Department of General Surgery, The People's Hospital of Ningdu County, Ganzhou, Jiangxi 342800, P.R. China, Department of General Surgery, The People's Hospital of Shangyou County, Ganzhou, Jiangxi 341200, P.R. China, Department of General Surgery, The Third Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi 341000, P.R. China, College of Pharmacy, Gannan Medical University, Ganzhou, Jiangxi 341000, P.R. China
Published online on: January 12, 2021 https://doi.org/10.3892/ol.2021.12461
Article Number: 200
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Increasing evidence has suggested an association between the expression profiles of microRNAs (miRs) and gallbladder cancer (GBC). Recently, miR‑182 has been demonstrated to exert tumor‑promoting effects. However, the biological activity and molecular mechanisms of miR‑182 in GBC remain unclear. The results of the present study demonstrated that miR‑182 expression was significantly upregulated in GBC tissues and cell lines (GBC‑SD and SGC‑996). In addition, miR‑182‑knockdown attenuated epithelial‑mesenchymal transition (EMT) in GBC cells, as indicated by decreased cell migratory and invasive abilities, decreased vimentin expression, and increased E‑cadherin expression. The activities of β‑catenin and its downstream factors, Cyclin D1 and c‑Myc, were also demonstrated to decrease following miR‑182‑knockdown. Forkhead box N3 (FOXN3) was identified as the direct target of miR‑182. Overexpression of FOXN3 ameliorated EMT and the β‑catenin pathway. Taken together, the results of the present study suggested that miR‑182 promotes EMT in GBC cells by targeting FOXN3, which suppresses the Wnt/β‑catenin pathway.

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