Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
March-2021 Volume 21 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
March-2021 Volume 21 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

MicroRNA‑182 promotes epithelial‑mesenchymal transition by targeting FOXN3 in gallbladder cancer

  • Authors:
    • Jianhong Zhang
    • Zeming Hu
    • Chao Wen
    • Qicheng Liao
    • Baoqing He
    • Jing Peng
    • Xin Tang
    • Zhixi Chen
    • Yuankang Xie
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary Surgery, The First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi 341000, P.R. China, Department of General Surgery, Zhejiang Xiaoshan Hospital, Hangzhou, Zhejiang 311202, P.R. China, School of Nursing, Gannan Medical University, Ganzhou, Jiangxi 341000, P.R. China, Department of General Surgery, The People's Hospital of Ningdu County, Ganzhou, Jiangxi 342800, P.R. China, Department of General Surgery, The People's Hospital of Shangyou County, Ganzhou, Jiangxi 341200, P.R. China, Department of General Surgery, The Third Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi 341000, P.R. China, College of Pharmacy, Gannan Medical University, Ganzhou, Jiangxi 341000, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 200
    |
    Published online on: January 12, 2021
       https://doi.org/10.3892/ol.2021.12461
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Increasing evidence has suggested an association between the expression profiles of microRNAs (miRs) and gallbladder cancer (GBC). Recently, miR‑182 has been demonstrated to exert tumor‑promoting effects. However, the biological activity and molecular mechanisms of miR‑182 in GBC remain unclear. The results of the present study demonstrated that miR‑182 expression was significantly upregulated in GBC tissues and cell lines (GBC‑SD and SGC‑996). In addition, miR‑182‑knockdown attenuated epithelial‑mesenchymal transition (EMT) in GBC cells, as indicated by decreased cell migratory and invasive abilities, decreased vimentin expression, and increased E‑cadherin expression. The activities of β‑catenin and its downstream factors, Cyclin D1 and c‑Myc, were also demonstrated to decrease following miR‑182‑knockdown. Forkhead box N3 (FOXN3) was identified as the direct target of miR‑182. Overexpression of FOXN3 ameliorated EMT and the β‑catenin pathway. Taken together, the results of the present study suggested that miR‑182 promotes EMT in GBC cells by targeting FOXN3, which suppresses the Wnt/β‑catenin pathway.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Wennmacker SZ, Lamberts MP, Di Martino M, Drenth JP, Gurusamy KS and van Laarhoven CJ: Transabdominal ultrasound and endoscopic ultrasound for diagnosis of gallbladder polyps. Cochrane Database Syst Rev. 8:CD0122332018.PubMed/NCBI

2 

Cubertafond P, Gainant A and Cucchiaro G: Surgical treatment of 724 carcinomas of the gallbladder. Results of the French Surgical Association Survey. Ann Surg. 219:275–280. 1994. View Article : Google Scholar : PubMed/NCBI

3 

Zali MR, Zamanian Azodi M, Razzaghi Z and Heydari MH: Gallbladder cancer integrated bioinformatics analysis of protein profile data. Gastroenterol Hepatol Bed Bench. 12 (Suppl 1):S66–S73. 2019.PubMed/NCBI

4 

Lee HE, Huh JW and Kim HS: Bioinformatics analysis of evolution and human disease related transposable element-derived microRNAs. Life (Basel). 10:952020.

5 

Lei R, Tang J, Zhuang X, Deng R, Li G, Yu J, Liang Y, Xiao J, Wang HY, Yang Q and Hu G: Suppression of MIM by microRNA-182 activates RhoA and promotes breast cancer metastasis. Oncogene. 33:1287–1296. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Yang MH, Yu J, Jiang DM, Li WL, Wang S and Ding YQ: microRNA-182 targets special AT-rich sequence-binding protein 2 to promote colorectal cancer proliferation and metastasis. J Transl Med. 12:1092014. View Article : Google Scholar : PubMed/NCBI

7 

Chandra V, Kim JJ, Mittal B and Rai R: MicroRNA aberrations: An emerging field for gallbladder cancer management. World J Gastroenterol. 22:1787–1799. 2016. View Article : Google Scholar : PubMed/NCBI

8 

Zhao M, Ang L, Huang J and Wang J: MicroRNAs regulate the epithelial-mesenchymal transition and influence breast cancer invasion and metastasis. Tumour Biol. 39:10104283176916822017. View Article : Google Scholar : PubMed/NCBI

9 

Li S, Liu F, Xu L, Li C, Yang X, Guo B, Gu J and Wang L: Wnt/β-catenin signaling axis is required for TFEB-mediated gastric cancer metastasis and epithelial-mesenchymal transition. Mol Cancer Res. 18:1650–1659. 2020. View Article : Google Scholar : PubMed/NCBI

10 

Wu L, Huang X, Li L, Huang H, Xu R and Luyten W: Insights on biology and pathology of HIF-1α/-2α, TGFβ/BMP, Wnt/β-catenin, and NF-κB pathways in osteoarthritis. Curr Pharm Des. 18:3293–3312. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Sánchez-Tilló E, de Barrios O, Siles L, Cuatrecasas M, Castells A and Postigo A: β-catenin/TCF4 complex induces the epithelial-to-mesenchymal transition (EMT)-activator ZEB1 to regulate tumor invasiveness. Proc Natl Acad Sci USA. 108:19204–19209. 2011. View Article : Google Scholar : PubMed/NCBI

12 

Zhao C, Mo L, Li C, Han S, Zhao W and Liu L: FOXN3 suppresses the growth and invasion of papillary thyroid cancer through the inactivation of Wnt/β-catenin pathway. Mol Cell Endocrinol. 515:1109252020. View Article : Google Scholar : PubMed/NCBI

13 

Chen J, Yu Y, Li H, Hu Q, Chen X, He Y, Xue C, Ren F, Ren Z, Li J, et al: Long non-coding RNA PVT1 promotes tumor progression by regulating the miR-143/HK2 axis in gallbladder cancer. Mol Cancer. 18:332019. View Article : Google Scholar : PubMed/NCBI

14 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

15 

Agarwal V, Bell GW, Nam JW and Bartel DP: Predicting effective microRNA target sites in mammalian mRNAs. Elife. 4:e050052015. View Article : Google Scholar

16 

Dai Y, Wang M, Wu H, Xiao M, Liu H and Zhang D: Loss of FOXN3 in colon cancer activates beta-catenin/TCF signaling and promotes the growth and migration of cancer cells. Oncotarget. 8:9783–9793. 2017. View Article : Google Scholar : PubMed/NCBI

17 

Kim GH, Fang XQ, Lim WJ, Park J, Kang TB, Kim JH and Lim JH: Cinobufagin suppresses melanoma cell growth by inhibiting LEF1. Int J Mol Sci. 21:67062020. View Article : Google Scholar

18 

Mishra SK, Kumari N and Krishnani N: Molecular pathogenesis of gallbladder cancer: An update. Mutat Res. 816-818:1116742019. View Article : Google Scholar : PubMed/NCBI

19 

Nakada S, Kuboki S, Nojima H, Yoshitomi H, Furukawa K, Takayashiki T, Takano S, Miyazaki M and Ohtsuka M: Roles of Pin1 as a key molecule for EMT induction by activation of STAT3 and NF-κB in human gallbladder cancer. Ann Surg Oncol. 26:907–917. 2019. View Article : Google Scholar : PubMed/NCBI

20 

Wang J, Jin Y, Li S, Song Q and Tang P: Identification of microRNAs associated with the survival of patients with gallbladder carcinoma. J Int Med Res. 48:3000605209180612020.PubMed/NCBI

21 

Wu K, Huang J, Xu T, Ye Z, Jin F, Li N and Lv B: MicroRNA-181b blocks gensenoside Rg3-mediated tumor suppression of gallbladder carcinoma by promoting autophagy flux via CREBRF/CREB3 pathway. Am J Transl Res. 11:5776–5787. 2019.PubMed/NCBI

22 

Lu K, Feng F, Yang Y, Liu K, Duan J, Liu H, Yang J, Wu M, Liu C and Chang Y: High-throughput screening identified miR-7-2-3p and miR-29c-3p as metastasis suppressors in gallbladder carcinoma. J Gastroenterol. 55:51–66. 2020. View Article : Google Scholar : PubMed/NCBI

23 

Cao MQ, You AB, Zhu XD, Zhang W, Zhang YY, Zhang SZ, Zhang KW, Cai H, Shi WK, Li XL, et al: miR-182-5p promotes hepatocellular carcinoma progression by repressing FOXO3a. J Hematol Oncol. 11:122018. View Article : Google Scholar : PubMed/NCBI

24 

Qiu Y, Luo X, Kan T, Zhang Y, Yu W, Wei Y, Shen N, Yi B and Jiang X: TGF-β upregulates miR-182 expression to promote gallbladder cancer metastasis by targeting CADM1. Mol Biosyst. 10:679–685. 2014. View Article : Google Scholar : PubMed/NCBI

25 

Charbonney E, Speight P, Masszi A, Nakano H and Kapus A: β-catenin and Smad3 regulate the activity and stability of myocardin-related transcription factor during epithelial-myofibroblast transition. Mol Biol Cell. 22:4472–4485. 2011. View Article : Google Scholar : PubMed/NCBI

26 

Zhou B, Liu Y, Kahn M, Ann DK, Han A, Wang H, Nguyen C, Flodby P, Zhong Q, Krishnaveni MS, et al: Interactions between β-catenin and transforming growth factor-β signaling pathways mediate epithelial-mesenchymal transition and are dependent on the transcriptional co-activator cAMP-response element-binding protein (CREB)-binding protein (CBP). J Biol Chem. 287:7026–7038. 2012. View Article : Google Scholar : PubMed/NCBI

27 

Taiyab A, Holms J and West-Mays JA: β-catenin/Smad3 interaction regulates transforming growth factor-β-induced epithelial to mesenchymal transition in the lens. Int J Mol Sci. 20:20782019. View Article : Google Scholar

28 

Li J, Chen Y, Han C, Huang S, Chen S, Luo L and Liu Y: JNK1/β-catenin axis regulates H2O2-induced epithelial-to-mesenchymal transition in human lens epithelial cells. Biochem Biophys Res Commun. 511:336–342. 2019. View Article : Google Scholar : PubMed/NCBI

29 

Liu D, Zhang H, Cui M, Chen C and Feng Y: Hsa-miR-425-5p promotes tumor growth and metastasis by activating the CTNND1-mediated β-catenin pathway and EMT in colorectal cancer. Cell Cycle. 19:1917–1927. 2020. View Article : Google Scholar : PubMed/NCBI

30 

Wang L, Hu Z, Guo Q, Yang L, Pang Y and Wang W: miR-23b functions as an oncogenic miRNA by downregulating Mcl-1S in lung cancer cell line A549. J Biochem Mol Toxicol. 34:e224942020. View Article : Google Scholar : PubMed/NCBI

31 

Yu J, Lei R, Zhuang X, Li X, Li G, Lev S, Segura MF, Zhang X and Hu G: MicroRNA-182 targets SMAD7 to potentiate TGFβ-induced epithelial-mesenchymal transition and metastasis of cancer cells. Nat Commun. 7:138842016. View Article : Google Scholar : PubMed/NCBI

32 

Kong X, Zhai J, Yan C, Song Y, Wang J, Bai X, Brown JAL and Fang Y: Recent advances in understanding FOXN3 in breast cancer, and other malignancies. Front Oncol. 9:2342019. View Article : Google Scholar : PubMed/NCBI

33 

Kirmizitas A, Meiklejohn S, Ciau-Uitz A, Stephenson R and Patient R: Dissecting BMP signaling input into the gene regulatory networks driving specification of the blood stem cell lineage. Proc Natl Acad Sci USA. 114:5814–5821. 2017. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhang J, Hu Z, Wen C, Liao Q, He B, Peng J, Tang X, Chen Z and Xie Y: MicroRNA‑182 promotes epithelial‑mesenchymal transition by targeting FOXN3 in gallbladder cancer. Oncol Lett 21: 200, 2021.
APA
Zhang, J., Hu, Z., Wen, C., Liao, Q., He, B., Peng, J. ... Xie, Y. (2021). MicroRNA‑182 promotes epithelial‑mesenchymal transition by targeting FOXN3 in gallbladder cancer. Oncology Letters, 21, 200. https://doi.org/10.3892/ol.2021.12461
MLA
Zhang, J., Hu, Z., Wen, C., Liao, Q., He, B., Peng, J., Tang, X., Chen, Z., Xie, Y."MicroRNA‑182 promotes epithelial‑mesenchymal transition by targeting FOXN3 in gallbladder cancer". Oncology Letters 21.3 (2021): 200.
Chicago
Zhang, J., Hu, Z., Wen, C., Liao, Q., He, B., Peng, J., Tang, X., Chen, Z., Xie, Y."MicroRNA‑182 promotes epithelial‑mesenchymal transition by targeting FOXN3 in gallbladder cancer". Oncology Letters 21, no. 3 (2021): 200. https://doi.org/10.3892/ol.2021.12461
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang J, Hu Z, Wen C, Liao Q, He B, Peng J, Tang X, Chen Z and Xie Y: MicroRNA‑182 promotes epithelial‑mesenchymal transition by targeting FOXN3 in gallbladder cancer. Oncol Lett 21: 200, 2021.
APA
Zhang, J., Hu, Z., Wen, C., Liao, Q., He, B., Peng, J. ... Xie, Y. (2021). MicroRNA‑182 promotes epithelial‑mesenchymal transition by targeting FOXN3 in gallbladder cancer. Oncology Letters, 21, 200. https://doi.org/10.3892/ol.2021.12461
MLA
Zhang, J., Hu, Z., Wen, C., Liao, Q., He, B., Peng, J., Tang, X., Chen, Z., Xie, Y."MicroRNA‑182 promotes epithelial‑mesenchymal transition by targeting FOXN3 in gallbladder cancer". Oncology Letters 21.3 (2021): 200.
Chicago
Zhang, J., Hu, Z., Wen, C., Liao, Q., He, B., Peng, J., Tang, X., Chen, Z., Xie, Y."MicroRNA‑182 promotes epithelial‑mesenchymal transition by targeting FOXN3 in gallbladder cancer". Oncology Letters 21, no. 3 (2021): 200. https://doi.org/10.3892/ol.2021.12461
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team