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IL10 hypomethylation is associated with the risk of gastric cancer

  • Authors:
    • Junjian Tang
    • Ranran Pan
    • Lele Xu
    • Qinghua Ma
    • Xiuru Ying
    • Jun Zhao
    • Haibin Zhao
    • Li Miao
    • Yue Xu
    • Shiwei Duan
    • Jinzhi Wang
  • View Affiliations / Copyright

    Affiliations: Department of Vascular Surgery, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu 214000, P.R. China, Medical Genetics Center, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, P.R. China, Department of Gastroenterology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215200, P.R. China, Department of Preventive Health, The Third People's Hospital of Xiangcheng District, Suzhou, Jiangsu 215134, P.R. China, Department of Pathology, Taihu Hospital, Wuxi, Jiangsu 214004, P.R. China, Department of Pediatrics, Xuzhou Medical University Affiliated Hospital of Lianyungang, Xuzhou, Jiangsu 222002, P.R. China, Department of Cell Biology, School of Medicine, Soochow University, Suzhou, Jiangsu 215007, P.R. China
    Copyright: © Tang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 241
    |
    Published online on: January 31, 2021
       https://doi.org/10.3892/ol.2021.12502
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Abstract

Interleukin‑10 (IL10), a pleiotropic cytokine secreted by type‑2 helper (Th2) T cells, contributes to the oncogenic activation or inactivation of tumor‑suppressor genes. The present study investigated whether hypomethylation of IL10 CpG island (CGI) was associated with the risk of developing gastric cancer (GC) and the prognosis of patients with GC. A fragment (hg18, chr1: 206945638‑206945774) at the CGI of IL10 was selected for the present methylation assay. Quantitative methylation‑specific PCR was used to evaluate the methylation of IL10 CGI in 117 tumor samples from patients with GC. The results demonstrated that IL10 CGI methylation was significantly lower in the tumor tissues compared with that in the paired adjacent non‑tumor tissues (median percentage of methylated reference, 29.16 vs. 42.82%, respectively; P=4x10‑8). Furthermore, results from receiver operating characteristic curve analysis identified a significant area under the curve of 0.706, with a sensitivity and a specificity of 77.8 and 58.1%, respectively, between cancer tissues and paired adjacent non‑tumor tissues. Furthermore, the methylation of IL10 CGI was significantly associated with patients' age at diagnosis (r=‑0.201; P=0.03). Subgroup analyses demonstrated that the association between IL10 CGI hypomethylation and the risk of GC was specific for patients with low differentiation (P=1x10‑7) and Borrmann types III+IV (P=1x10‑7). In addition, IL10 CGI hypomethylation was significantly associated with the risk of GC for patients without smoking history (P=3x10‑7) or a family history of cancer (P=2x10‑7). The results from Kaplan‑Meier survival analysis demonstrated that IL10 CGI hypomethylation was associated with a significantly shorter overall survival of patients with GC (P=0.041). Similar results were identified for patients with GC who did not have smoking history (P=0.037) or a family history of cancer (P=0.049). The results from this study demonstrated that IL10 CGI hypomethylation may be considered as a potential biomarker for the diagnosis and prognosis of patients with GC in the Chinese population.
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Copy and paste a formatted citation
Spandidos Publications style
Tang J, Pan R, Xu L, Ma Q, Ying X, Zhao J, Zhao H, Miao L, Xu Y, Duan S, Duan S, et al: IL10 hypomethylation is associated with the risk of gastric cancer. Oncol Lett 21: 241, 2021.
APA
Tang, J., Pan, R., Xu, L., Ma, Q., Ying, X., Zhao, J. ... Wang, J. (2021). IL10 hypomethylation is associated with the risk of gastric cancer. Oncology Letters, 21, 241. https://doi.org/10.3892/ol.2021.12502
MLA
Tang, J., Pan, R., Xu, L., Ma, Q., Ying, X., Zhao, J., Zhao, H., Miao, L., Xu, Y., Duan, S., Wang, J."IL10 hypomethylation is associated with the risk of gastric cancer". Oncology Letters 21.4 (2021): 241.
Chicago
Tang, J., Pan, R., Xu, L., Ma, Q., Ying, X., Zhao, J., Zhao, H., Miao, L., Xu, Y., Duan, S., Wang, J."IL10 hypomethylation is associated with the risk of gastric cancer". Oncology Letters 21, no. 4 (2021): 241. https://doi.org/10.3892/ol.2021.12502
Copy and paste a formatted citation
x
Spandidos Publications style
Tang J, Pan R, Xu L, Ma Q, Ying X, Zhao J, Zhao H, Miao L, Xu Y, Duan S, Duan S, et al: IL10 hypomethylation is associated with the risk of gastric cancer. Oncol Lett 21: 241, 2021.
APA
Tang, J., Pan, R., Xu, L., Ma, Q., Ying, X., Zhao, J. ... Wang, J. (2021). IL10 hypomethylation is associated with the risk of gastric cancer. Oncology Letters, 21, 241. https://doi.org/10.3892/ol.2021.12502
MLA
Tang, J., Pan, R., Xu, L., Ma, Q., Ying, X., Zhao, J., Zhao, H., Miao, L., Xu, Y., Duan, S., Wang, J."IL10 hypomethylation is associated with the risk of gastric cancer". Oncology Letters 21.4 (2021): 241.
Chicago
Tang, J., Pan, R., Xu, L., Ma, Q., Ying, X., Zhao, J., Zhao, H., Miao, L., Xu, Y., Duan, S., Wang, J."IL10 hypomethylation is associated with the risk of gastric cancer". Oncology Letters 21, no. 4 (2021): 241. https://doi.org/10.3892/ol.2021.12502
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