Open Access

Dipyridamole induces the phosphorylation of CREB to promote cancer cell proliferation

  • Authors:
    • Lina Abdelghany
    • Nageh El-Μahdy
    • Tsuyoshi Kawabata
    • Shinji Goto
    • Tao-Sheng Li
  • View Affiliations

  • Published online on: February 3, 2021     https://doi.org/10.3892/ol.2021.12512
  • Article Number: 251
  • Copyright: © Abdelghany et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Dipyridamole, a traditional anti‑platelet drug, has been reported to inhibit the proliferation of cancer cells. The present study aimed to investigate the possibility of dipyridamole as an adjuvant of chemotherapy by enhancing the cytotoxicity of an anti‑cancer drug. The cytotoxicity of colorectal cancer cells (HCT‑8), CD133+/CD44+ stem‑like subpopulation of HCT‑8 cells and lymphoma cells (U937) to dipyridamole and/or doxorubicin was evaluated using MTT proliferation and colony forming assays. The expression levels of phosphorylated cAMP‑regulatory element‑binding protein (pCREB) and poly(ADP‑ribose) polymerase‑1 (PARP‑1) in cells were analyzed via western blotting and immunofluorescence. The present study reported controversial data regarding the anti‑cancer effect of dipyridamole. Dipyridamole increased, rather than inhibited, the proliferation of HCT‑8 and U937 cells in a dose‑dependent manner. Furthermore, it was found that dipyridamole significantly increased the expression levels of pCREB and PARP‑1. However, the combined usage of dipyridamole significantly enhanced the cytotoxicity of doxorubicin to HCT‑8 cells at particular doses. Based on the current findings, dipyridamole likely induces the phosphorylation of CREB to promote the proliferation of cancer cells, but may enhance the cytotoxicity of anti‑cancer drugs at particular doses.
View Figures
View References

Related Articles

Journal Cover

April-2021
Volume 21 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Abdelghany L, El-Μahdy N, Kawabata T, Goto S and Li T: Dipyridamole induces the phosphorylation of CREB to promote cancer cell proliferation. Oncol Lett 21: 251, 2021
APA
Abdelghany, L., El-Μahdy, N., Kawabata, T., Goto, S., & Li, T. (2021). Dipyridamole induces the phosphorylation of CREB to promote cancer cell proliferation. Oncology Letters, 21, 251. https://doi.org/10.3892/ol.2021.12512
MLA
Abdelghany, L., El-Μahdy, N., Kawabata, T., Goto, S., Li, T."Dipyridamole induces the phosphorylation of CREB to promote cancer cell proliferation". Oncology Letters 21.4 (2021): 251.
Chicago
Abdelghany, L., El-Μahdy, N., Kawabata, T., Goto, S., Li, T."Dipyridamole induces the phosphorylation of CREB to promote cancer cell proliferation". Oncology Letters 21, no. 4 (2021): 251. https://doi.org/10.3892/ol.2021.12512