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lncRNA XIST regulates cell proliferation, migration and invasion via regulating miR‑30b and RECK in nasopharyngeal carcinoma

  • Authors:
    • Lingling Sun
    • Min Zhang
    • Hongxia Qu
  • View Affiliations / Copyright

    Affiliations: Department of Otorhinolaryngology Head and Neck Surgery, Liaocheng People's Hospital, Liaocheng, Shandong 25200, P.R. China, Department of Radiology, Liaocheng People's Hospital, Liaocheng, Shandong 25200, P.R. China
    Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 256
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    Published online on: February 4, 2021
       https://doi.org/10.3892/ol.2021.12513
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Abstract

Long non‑coding RNA (lncRNA) X‑inactive specific transcript (XIST) plays an essential role in the development and progress of nasopharyngeal carcinoma (NPC). MicroRNA‑30b (miR‑30b) has been confirmed to play an inhibitory role in various types of cancer. The molecular mechanisms underlying the lncRNA XIST‑mediated regulation of the metastasis of NPC cells by miR‑30b is not clear. qPCR and western blot analysis were used to detect the expression of XIST, miR‑30b, and reversion inducing cysteine rich protein with kazal motifs (RECK) in NPC tissues and cell lines. The detection of luciferase reporter gene confirmed the relationship between lncRNA XIST, miR‑30b and RECK. CCK‑8 and Transwell assays were performed in order to detect the proliferation, migration and invasion of the NPC cells. The results of qPCR and western blotting indicated that the expression levels of lncRNA XIST and RECK were higher in the NPC tissues and cell lines than that of the control group, while the expression of miR‑30b was lower. Knockdown of lncRNA XIST significantly inhibited cell proliferation, migration and invasion in the NPC cell lines. In addition, lncRNA XIST was found to negatively regulate the expression of miR‑30b, resulting in the upregulation of RECK. Overexpression of RECK was found to reverse the inhibitory effect of lncRNA XIST knockdown or miR‑30b on NPC cell metastasis. Our results showed that cell migration and invasion were inhibited by knockdown of lncRNA XIST, suggesting that the lncRNA XIST/miR‑30b/RECK axis is involved in the development of NPC.
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Copy and paste a formatted citation
Spandidos Publications style
Sun L, Zhang M and Qu H: lncRNA XIST regulates cell proliferation, migration and invasion via regulating miR‑30b and RECK in nasopharyngeal carcinoma. Oncol Lett 21: 256, 2021.
APA
Sun, L., Zhang, M., & Qu, H. (2021). lncRNA XIST regulates cell proliferation, migration and invasion via regulating miR‑30b and RECK in nasopharyngeal carcinoma. Oncology Letters, 21, 256. https://doi.org/10.3892/ol.2021.12513
MLA
Sun, L., Zhang, M., Qu, H."lncRNA XIST regulates cell proliferation, migration and invasion via regulating miR‑30b and RECK in nasopharyngeal carcinoma". Oncology Letters 21.4 (2021): 256.
Chicago
Sun, L., Zhang, M., Qu, H."lncRNA XIST regulates cell proliferation, migration and invasion via regulating miR‑30b and RECK in nasopharyngeal carcinoma". Oncology Letters 21, no. 4 (2021): 256. https://doi.org/10.3892/ol.2021.12513
Copy and paste a formatted citation
x
Spandidos Publications style
Sun L, Zhang M and Qu H: lncRNA XIST regulates cell proliferation, migration and invasion via regulating miR‑30b and RECK in nasopharyngeal carcinoma. Oncol Lett 21: 256, 2021.
APA
Sun, L., Zhang, M., & Qu, H. (2021). lncRNA XIST regulates cell proliferation, migration and invasion via regulating miR‑30b and RECK in nasopharyngeal carcinoma. Oncology Letters, 21, 256. https://doi.org/10.3892/ol.2021.12513
MLA
Sun, L., Zhang, M., Qu, H."lncRNA XIST regulates cell proliferation, migration and invasion via regulating miR‑30b and RECK in nasopharyngeal carcinoma". Oncology Letters 21.4 (2021): 256.
Chicago
Sun, L., Zhang, M., Qu, H."lncRNA XIST regulates cell proliferation, migration and invasion via regulating miR‑30b and RECK in nasopharyngeal carcinoma". Oncology Letters 21, no. 4 (2021): 256. https://doi.org/10.3892/ol.2021.12513
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