T cell immune regulator 1 is a prognostic marker associated with immune infiltration in glioblastoma multiforme

Qi,Chunxiao; Qi,Chunxiao; Qi,Chunxiao; Qi,Chunxiao; Lei,Lei; Lei,Lei; Lei,Lei; Lei,Lei; Hu,Jinqu; Hu,Jinqu; Hu,Jinqu; Hu,Jinqu; Wang,Gang; Wang,Gang; Wang,Gang; Wang,Gang; Liu,Jiyuan; Liu,Jiyuan; Liu,Jiyuan; Liu,Jiyuan; Ou,Shaowu; Ou,Shaowu; Ou,Shaowu; Ou,Shaowu

doi:10.3892/ol.2021.12514

T cell immune regulator 1 is a prognostic marker associated with immune infiltration in glioblastoma multiforme

Authors:
- Chunxiao Qi
- Lei Lei
- Jinqu Hu
- Gang Wang
- Jiyuan Liu
- Shaowu Ou
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Affiliations: Department of Neurosurgery, The First Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China, Department of Rheumatology and Immunology, Dalian Municipal Central Hospital Affiliated of Dalian Medical University, Dalian, Liaoning 116033, P.R. China
Published online on: February 3, 2021 https://doi.org/10.3892/ol.2021.12514
Article Number: 252
Copyright: © Qi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Glioma is the most common primary brain tumor and glioblastoma multiforme (GBM) is the most malignant brain glioma with the worst prognosis. T cell immune regulator 1 (TCIRG1) constitutes the V₀a₃ subunit of vacuolar ATPase (V‑ATPase), and the function of V‑ATPase in malignant tumors, such as breast cancer, melanoma and hepatocellular carcinoma, has been reported. However, the effect of the TCIRG1 subunit on GBM remains to be fully elucidated. mRNA levels of TCIRG1 in different cancer types and the corresponding normal tissues were extracted from the Oncomine and Tumor Immune Estimation Resource (TIMER) databases. The Gene Expression Omnibus (access number: GSE16011), the Chinese Glioma Genome Atlas and The Cancer Genome Atlas were used to investigate the mRNA level of TCIRG1 in glioma. Protein level validation in glioma was performed using western blotting. The Database for Annotation, Visualization and Integrated Discovery was used to analyze Gene Ontology (GO) categories for genes correlated with TCIRG1 in GBM. Protein‑protein interaction (PPI) networks and module analyses were performed using Cytoscape software and the MCODE plugin. The correlation between tumor immune cell infiltration and TCIRG1 expression was explored using the TIMER database. Additionally, the correlation between TCIRG1 and the gene signature of immune infiltration was explored through TIMER and Gene Expression Profiling Interactive Analysis. External validation of TCIRG1 expression according to immune signatures in GBM was performed using the GSE16011 dataset with the GlioVis online tool. It was found that TCIRG1 expression was increased in GBM and numerous malignant tumors and may serve as a biomarker of the mesenchymal subtype of GBM. GO category analysis of positively correlated genes revealed that TCIRG1 was correlated with the immune response in GBM. PPI network and module analyses also supported the potential function of TCIRG1 in the local immune response. The expression of TCIRG1 was associated with various immune markers. It was therefore speculated that TCIRG1 is associated with glioma malignancy and may be a marker of unfavorable prognosis in patients with GBM, and it could be regarded as a prognostic biomarker and an indicator of immune infiltration in GBM.

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