MicroRNA‑124 suppresses the invasion and proliferation of breast cancer cells by targeting TFAP4

Cha,Nier; Cha,Nier; Jia,Baoqing; Jia,Baoqing; He,Yinzai; He,Yinzai; Luan,Wei; Luan,Wei; Bao,Wenhua; Bao,Wenhua; Han,Xiuhua; Han,Xiuhua; Gao,Weishi; Gao,Weishi; Gao,Yanwei; Gao,Yanwei

doi:10.3892/ol.2021.12532

MicroRNA‑124 suppresses the invasion and proliferation of breast cancer cells by targeting TFAP4

Authors:
- Nier Cha
- Baoqing Jia
- Yinzai He
- Wei Luan
- Wenhua Bao
- Xiuhua Han
- Weishi Gao
- Yanwei Gao
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Affiliations: Department of Surgical Oncology, Inner Mongolia People's Hospital, Hohhot, Inner Mongolia 010017, P.R. China, Department of Medical Oncology, Inner Mongolia People's Hospital, Hohhot, Inner Mongolia 010017, P.R. China
Published online on: February 9, 2021 https://doi.org/10.3892/ol.2021.12532
Article Number: 271
Copyright: © Cha et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNA (miRNA/miR)‑124 is widely accepted as the suppressor of different tumors. The present study aimed to improve understanding of the potential role of miR‑124 in breast cancer. The gene expression profile change derived from the overexpression of miR‑124 was investigated using RNA sequencing and bioinformatics analysis of the breast cancer cell line SKBR3. The results demonstrated that the gene expression profile of SKBR3 cells significantly changed. In addition, the transcription factor activating enhancer‑binding protein 4 (TFAP4) gene was identified among the top 10 differentially expressed genes, and was identified as a novel target gene of miR‑124 using a dual‑luciferase reporter assay. TFAP4 knockdown in notably impaired SKBR3 cell migration and proliferation, which was consistent with decreasing migration and proliferation ability following overexpression of miR‑124. Taken together, these results suggest that overexpression of miR‑124 can suppress the migration and proliferation of SKBR3 cells by tarsgeting TFAP4. Thus, TFAP4 may act as a novel therapeutic target of breast cancer.

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