Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
April-2021 Volume 21 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
April-2021 Volume 21 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML

  • Supplementary Files
    • Supplementary_Data.pdf
Article Open Access

MicroRNA‑29a inhibits cell proliferation and arrests cell cycle by modulating p16 methylation in cervical cancer

  • Authors:
    • Anjin Wang
    • Qiying Xu
    • Rengaowa Sha
    • Tonghui Bao
    • Xiaoli Xi
    • Guilan Guo
  • View Affiliations / Copyright

    Affiliations: School of Basic Medical Sciences, Wuhan University, Wuhan, Hubei 430072, P.R. China, Department of Gynecology, Affiliated Hospital of Qinghai University, Xining, Qinghai 810100, P.R. China, Department of Medicine, Qinghai University, Xining, Qinghai 810016, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 272
    |
    Published online on: February 9, 2021
       https://doi.org/10.3892/ol.2021.12533
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Cervical cancer is the second most common gynecological malignancy. Accumulating evidence has suggested that microRNAs (miRNAs) are involved in the occurrence and development of cervical cancer. The present study aimed to investigate the function and underlying molecular mechanism of microRNA (miRNA/miR)‑29a in cervical cancer. Reverse transcription‑quantitative PCR and methylation‑specific PCR were used to examine the expression of miR‑29a and methylated status of p16 promoter, respectively. Cell Counting Kit‑8 analysis and flow cytometry were performed to evaluate cell viability and cycle, respectively. Dual‑luciferase reporter assay was performed to verify the interaction between miR‑29a and its targets. Western blot analysis was performed to detect the protein levels of DNA methyltransferases (DNMT)3A and DNMT3B. The results demonstrated that miR‑29a expression was downregulated in cervical cancer tissues and cells, and negatively correlated with p16 promoter hypermethylation. Furthermore, cell experiments confirmed that miR‑29a suppressed cell proliferation and induced cell cycle arrest in HeLa and C‑33A cells. Mechanically, miR‑29a restored normal methylation pattern of the p16 gene by sponging DNMT3A and DNMT3B. Taken together, the results of the present study demonstrated the epigenetic regulation of tumor suppressor p16 by miR‑29a as a unique mechanism, thus providing a rationale for the development of miRNA‑based strategies in the treatment of cervical cancer.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Freddie B, Ferlay J, Soerjomataram I, Siegel RL, Torre LA and Jemal A: Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 68:394–424. 2018. View Article : Google Scholar : PubMed/NCBI

2 

Razavi ZS, Tajiknia V, Majidi S, Ghandali M, Mirzaei HR, Rahimian N, Hamblin MR and Mirzaei H: Gynecologic cancers and non-coding RNAs: Epigenetic regulators with emerging roles. Crit Rev Oncol Hematol. 157:1031922020. View Article : Google Scholar : PubMed/NCBI

3 

Burd EM: Human papillomavirus and cervical cancer. Clin Microbiol Rev. 16:1–17. 2003. View Article : Google Scholar : PubMed/NCBI

4 

Menezo Y, Clement P, Clement A and Elder K: Methylation: An ineluctable biochemical and physiological process essential to the transmission of life. Int J Mol Sci. 21:93112020. View Article : Google Scholar

5 

Zhu H, Zhu H, Tian M, Wang D, He J and Xu T: DNA methylation and hydroxymethylation in cervical cancer: Diagnosis, prognosis and treatment. Front Genet. 9:3472020. View Article : Google Scholar

6 

Urbano A, Smith J, Weeks RJ and Chatterjee A: Gene-Specific targeting of DNA methylation in the mammalian genome. Cancers (Basel). 11:15152019. View Article : Google Scholar

7 

Jiao Y, Feng Y and Wang X: Regulation of tumor suppressor gene CDKN2A and Encoded p16-INK4a protein by covalent modifications. Biochemistry (Mosc). 83:1289–1298. 2018. View Article : Google Scholar : PubMed/NCBI

8 

Wang FL, Yang Y, Liu ZY, Qin Y and Jin T: Correlation between methylation of the p16 promoter and cervical cancer incidence. Eur Rev Med Pharmacol Sci. 21:2351–2356. 2017.PubMed/NCBI

9 

O'Neill CJ and McCluggage WG: p16 expression in the female genital tract and its value in diagnosis. Adv Anat Pathol. 13:8–15. 2006. View Article : Google Scholar : PubMed/NCBI

10 

Han YD, Wang XB, Cui NH, Zhang S, Wang C and Zheng F: Associations of P16INK4a promoter hypermethylation with squamous intra-epithelial lesion, cervical cancer and their clinicopathological features: A meta-analysis. Oncotarget. 8:1871–1883. 2017. View Article : Google Scholar : PubMed/NCBI

11 

Kohler F and Rodríguez-Paredes M: DNA methylation in epidermal differentiation, aging, and cancer. J Invest Dermatol. 140:38–47. 2020. View Article : Google Scholar : PubMed/NCBI

12 

Gujar H, Weisenberger DJ and Liang G: The roles of human DNA methyltransferases and their isoforms in shaping the epigenome. Genes (Basel). 10:1722019. View Article : Google Scholar

13 

Hassouna MM, Naguib M, Radwan EM, Abdel-Samiee M, Estaphan S and Abdelsameea E: DNA methyltransferases as potential biomarkers for HCV related hepatocellular carcinoma. Asian Pac J Cancer Prev. 1:3357–3363. 2020. View Article : Google Scholar

14 

Zhu A, Hopkins KM, Friedman RA, Bernstock JD, Broustas CG and Lieberman HB: DNMT1 and DNMT3B regulate tumorigenicity of human prostate cancer cells by controlling RAD9 expression through targeted methylation. Carcinogenesis. 11:bgaa0882020. View Article : Google Scholar

15 

Cervena K, Siskova A, Buchler T, Vodicka P and Vymetalkova V: Methylation-based therapies for colorectal cancer. Cells. 24:15402020. View Article : Google Scholar

16 

Liu H, Song Y, Qiu H, Liu Y, Luo K, Yi Y, Jiang G, Lu M, Zhang Z, Yin J, et al: Downregulation of FOXO3a by DNMT1 promotes breast cancer stem cell properties and tumorigenesis. Cell Death Differ. 27:966–983. 2020. View Article : Google Scholar : PubMed/NCBI

17 

Piyathilake CJ, Badiga S, Borak SG, Weragoda J, Bae S, Matthews R, Bell WC and Partridge EE: A higher degree of expression of DNA methyl transferase 1 in cervical cancer is associated with poor survival outcome. Int J Womens Health. 9:413–420. 2017. View Article : Google Scholar : PubMed/NCBI

18 

Sundaram MK, Hussain A, Haque S, Raina R and Afroze N: Quercetin modifies 5′CpG promoter methylation and reactivates various tumor suppressor genes by modulating epigenetic marks in human cervical cancer cells. J Cell Biochem. 120:18357–18369. 2019. View Article : Google Scholar : PubMed/NCBI

19 

Sundaram MK, Ansari MZ, Al Mutery A, Ashraf M, Nasab R, Rai S, Rais N and Hussain A: Genistein induces alterations of epigenetic modulatory signatures in human cervical cancer cells. Anticancer Agents Med Chem. 18:412–421. 2018. View Article : Google Scholar : PubMed/NCBI

20 

Khan MA, Sundaram MK, Hamza A, Quraishi U, Gunasekera D, Ramesh L, Goala P, Al Alami U, Ansari MZ, Rizvi TA, et al: Sulforaphane reverses the expression of various tumor suppressor genes by targeting DNMT3B and HDAC1 in human cervical cancer cells. Evid Based Complement Alternat Med. 2015:4121492015.PubMed/NCBI

21 

Lu TX and Rothenberg ME: MicroRNA. J Allergy Clin Immunol. 141:1202–1207. 2018. View Article : Google Scholar : PubMed/NCBI

22 

Kabekkodu SP, Shukla V, Varghese VK, D'Souza J, Chakrabarty S and Satyamoorthy K: Clustered miRNAs and their role in biological functions and diseases. Biol Rev Camb Philos Soc. 93:1955–1986. 2018. View Article : Google Scholar : PubMed/NCBI

23 

Condrat CE, Thompson DC, Barbu MG, Bugnar OL, Boboc A, Cretoiu D, Suciu N, Cretoiu SM and Voinea SC: miRNAs as biomarkers in disease: Latest findings regarding their role in diagnosis and prognosis. Cells. 9:2762020. View Article : Google Scholar

24 

Yang Y, Dodbele S, Park T, Glass R, Bhat K, Sulman EP, Zhang Y and Abounader R: MicroRNA-29a inhibits glioblastoma stem cells and tumor growth by regulating the PDGF pathway. J Neurooncol. 145:23–34. 2019. View Article : Google Scholar : PubMed/NCBI

25 

Liu YB, Wang Y, Zhang MD, Yue W and Sun CN: MicroRNA-29a functions as a tumor suppressor through targeting STAT3 in laryngeal squamous cell carcinoma. Exp Mol Pathol. 116:1045212020. View Article : Google Scholar : PubMed/NCBI

26 

Gong HL, Tao Y, Mao XZ, Song DY, You D and Ni JD: MicroRNA-29a suppresses the invasion and migration of osteosarcoma cells by regulating the SOCS1/NF-κB signalling pathway through negatively targeting DNMT3B. Int J Mol Med. 44:1219–1232. 2019.PubMed/NCBI

27 

Nan P, Niu Y, Wang X and Li Q: miR-29a function as tumor suppressor in cervical cancer by targeting SIRT1 and predict patient prognosis. Onco Targets Ther. 26:6917–6925. 2019. View Article : Google Scholar

28 

Zhang Y, Yang L, Wang S, Liu Z and Xiu M: miR-29a suppresses cell proliferation by targeting SIRT1 in hepatocellular carcinoma. Cancer Biomark. 22:151–159. 2018. View Article : Google Scholar : PubMed/NCBI

29 

Liu ZJ, Chen SG, Yang YZ, Lu SJ, Zhao XM, Hu B and Zhang L: miR-29a inhibits adhesion, migration, and invasion of osteosarcoma cells by suppressing CDC42. Int J Clin Exp Pathol. 12:4171–4180. 2019.PubMed/NCBI

30 

Chen R and Zhang L: miR-29a inhibits cell proliferation and migration by targeting the CDC42/PAK1 signaling pathway in cervical cancer. Anticancer Drugs. 30:579–587. 2019. View Article : Google Scholar : PubMed/NCBI

31 

Shi C, Ren L, Sun C, Yu L, Bian X, Zhou X, Wen Y, Hua D, Zhao S, Luo W, et al: miR-29a/b/c function as invasion suppressors for gliomas by targeting CDC42 and predict the prognosis of patients. Br J Cancer. 117:1036–1047. 2017. View Article : Google Scholar : PubMed/NCBI

32 

Zhang M, Guo W, Qian J and Wang B: Negative regulation of CDC42 expression and cell cycle progression by miR-29a in breast cancer. Open Med (Wars). 11:78–82. 2016. View Article : Google Scholar : PubMed/NCBI

33 

Yamamoto N, Kinoshita T, Nohata N, Yoshino H, Itesako T, Fujimura L, Mitsuhashi A, Usui H, Enokida H, Nakagawa M, et al: Tumor-suppressive microRNA-29a inhibits cancer cell migration and invasion via targeting HSP47 in cervical squamous cell carcinoma. Int J Oncol. 43:1855–1863. 2013. View Article : Google Scholar : PubMed/NCBI

34 

Bibaki E, Tsitoura E, Vasarmidi E, Margaritopoulos G, Trachalaki A, Koutoulaki C, Georgopoulou T, Spandidos DA, Tzanakis N and Antoniou KM: miR-185 and miR-29a are similarly expressed in the bronchoalveolar lavage cells in IPF and lung cancer but common targets DNMT1 and COL1A1 show disease specific patterns. Mol Med Rep. 17:7105–7112. 2018.PubMed/NCBI

35 

Kogure T, Kondo Y, Kakazu E, Ninomiya M, Kimura O and Shimosegawa T: Involvement of miRNA-29a in epigenetic regulation of transforming growth factor-beta-induced epithelial-mesenchymal transition in hepatocellular carcinoma. Hepatol Res. 44:907–919. 2014. View Article : Google Scholar : PubMed/NCBI

36 

Cicchini C, de Nonno V, Battistelli C, Cozzolino AM, De Santis Puzzonia M, Ciafrè SA, Brocker C, Gonzalez FJ, Amicone L and Tripodi M: Epigenetic control of EMT/MET dynamics: HNF4α impacts DNMT3s through miRs-29. Biochim Biophys Acta. 1849:919–929. 2015. View Article : Google Scholar : PubMed/NCBI

37 

Robaina MC, Mazzoccoli L, Arruda VO, de Souza Reis FD, Apa AG, de Rezende LM and Klumb CE: Deregulation of DNMT1, DNMT3B and miR-29s in burkitt lymphoma suggests novel contribution for disease pathogenesis. Exp Mol Pathol. 98:200–207. 2015. View Article : Google Scholar : PubMed/NCBI

38 

Oliveira LH, Schiavinato JL, Fráguas MS, Lucena-Araujo AR, Haddad R, Araújo AG, Dalmazzo LF, Rego EM, Covas DT, Zago MA and Panepucci RA: Potential roles of microRNA-29a in the molecular pathophysiology of T-cell acute lymphoblastic leukemia. Cancer Sci. 106:1264–1277. 2015. View Article : Google Scholar : PubMed/NCBI

39 

Zhou X, Zhao F, Wang ZN, Song YX, Chang H, Chiang Y and Xu HM: Altered expression of miR-152 and miR-148a in ovarian cancer is related to cell proliferation. Oncol Rep. 27:447–454. 2012.PubMed/NCBI

40 

Vasaikar SV, Straub P, Wang J and Zhang B: LinkedOmics: Analyzing multi-omics data within and across 32 cancer types. Nucleic Acids Res. 46:D956–D963. 2018. View Article : Google Scholar : PubMed/NCBI

41 

Gao L, Anteneh H and Song J: Dissect the DNMT3A- and DNMT3B-mediated DNA Co-methylation through a covalent complex approach. J Mol Biol. 17:569–575. 2020. View Article : Google Scholar

42 

Di Leva G, Garofalo M and Croce CM: MicroRNAs in cancer. Annu Rev Pathol. 9:287–314. 2014. View Article : Google Scholar : PubMed/NCBI

43 

Chen X, Li M, Zhou H and Zhang L: miR-132 Targets FOXA1 and exerts tumor-suppressing functions in thyroid cancer. Oncol Res. 29:431–437. 2019. View Article : Google Scholar

44 

Wang JY and Chen LJ: The role of miRNAs in the invasion and metastasis of cervical cancer. Biosci Rep. 15:BSR201813772019. View Article : Google Scholar

45 

Li J, Liu Q, Clark LH, Qiu H, Bae-Jump VL and Zhou C: Deregulated miRNAs in human cervical cancer: Functional importance and potential clinical use. Future Oncol. 13:743–753. 2017. View Article : Google Scholar : PubMed/NCBI

46 

Wang Y, Han J, Lv Y and Zhang G: miR-29a inhibits proliferation, invasion, and migration of papillary thyroid cancer by targeting DPP4. Onco Targets Ther. 12:4225–4233. 2019. View Article : Google Scholar : PubMed/NCBI

47 

Huang C, Wang L, Song H and Wu C: miR-29a inhibits the progression of oral squamous cell carcinoma by targeting wnt/β-catenin signalling pathway. Artif Cells Nanomed Biotechnol. 47:3037–3042. 2019. View Article : Google Scholar : PubMed/NCBI

48 

Liu X, Lv X, Yang Q, Jin H, Zhou W and Fan Q: MicroRNA-29a functions as a tumor suppressor and increases cisplatin sensitivity by targeting NRAS in lung cancer. Technol Cancer Res Treat. 17:15330338187589052018. View Article : Google Scholar : PubMed/NCBI

49 

Li Y, Wang F, Xu J, Ye F, Shen Y, Zhou J, Lu W, Wan X, Ma D and Xie X: Progressive miRNA expression profiles in cervical carcinogenesis and identification of HPV-related target genes for miR-29. J Pathol. 224:484–495. 2011. View Article : Google Scholar : PubMed/NCBI

50 

Zhu Y, Huang Y, Liu M, Yan Q, Zhao W, Yang P, Gao Q, Wei J, Zhao W and Ma L: Epigallocatechin gallate inhibits cell growth and regulates miRNA expression in cervical carcinoma cell lines infected with different high-risk human papillomavirus subtypes. Exp Ther Med. 17:1742–1748. 2019.PubMed/NCBI

51 

Gong Y, Wan JH, Zou W, Lian GY, Qin JL and Wang QM: miR-29a inhibits invasion and metastasis of cervical cancer via modulating methylation of tumor suppressor SOCS1. Future Oncol. 15:1729–1744. 2019. View Article : Google Scholar : PubMed/NCBI

52 

Zhang CY, Bao W and Wang LH: Downregulation of p16(ink4a) inhibits cell proliferation and induces G1 cell cycle arrest in cervical cancer cells. Int J Mol Med. 33:1577–1585. 2014. View Article : Google Scholar : PubMed/NCBI

53 

Suzuki N, Onda T, Yamamoto N, Katakura A, Mizoe Je and Shibahara T: Mutation of the p16/CDKN2 gene and loss of heterozygosity in malignant mucosal melanoma and adenoid cystic carcinoma of the head and neck. Int J Oncol. 31:1061–1067. 2007.PubMed/NCBI

54 

Aesif SW, Aubry MC, Yi ES, Kloft-Nelson SM, Jenkins SM, Spears GM, Greipp PT, Sukov WR and Roden AC: Loss of p16INK4A expression and homozygous CDKN2A deletion are associated with worse outcome and younger age in thymic carcinomas. J Thorac Oncol. 12:860–871. 2017. View Article : Google Scholar : PubMed/NCBI

55 

Demokan S, Chuang A, Suoğlu Y, Ulusan M, Yalnız Z, Califano JA and Dalay N: Promoter methylation and loss of p16(INK4a) gene expression in head and neck cancer. Head Neck. 34:1470–1475. 2012. View Article : Google Scholar : PubMed/NCBI

56 

Allameh A, Moazeni-Roodi A, Harirchi I, Ravanshad M, Motiee-Langroudi M, Garajei A, Hamidavi A and Mesbah-Namin SA: Promoter DNA methylation and mRNA expression level of p16 gene in oral squamous cell carcinoma: Correlation with clinicopathological characteristics. Pathol Oncol Res. 25:1535–1543. 2019. View Article : Google Scholar : PubMed/NCBI

57 

Zhang ZM, Lu R, Wang P, Yu Y, Chen D, Gao L, Liu S, Ji D, Rothbart SB, Wang Y, et al: Structural basis for DNMT3A-mediated de novo DNA methylation. Nature. 554:387–391. 2018. View Article : Google Scholar : PubMed/NCBI

58 

Jin B and Robertson KD: DNA methyltransferases, DNA damage repair, and cancer. Adv Exp Med Biol. 754:3–29. 2013. View Article : Google Scholar : PubMed/NCBI

59 

Liu D, Wu K, Yang Y, Zhu D, Zhang C and Zhao S: Long noncoding RNA ADAMTS9-AS2 suppresses the progression of esophageal cancer by mediating CDH3 promoter methylation. Mol Carcinog. 59:32–44. 2020. View Article : Google Scholar : PubMed/NCBI

60 

de Silanes IL, Gorospe M, Taniguchi H, Abdelmohsen K, Srikantan S, Alaminos M, Berdasco M, Urdinguio RG, Fraga MF, Jacinto FV and Esteller M: The RNA-binding protein HuR regulates DNA methylation through stabilization of DNMT3b mRNA. Nucleic Acids Res. 37:2658–2671. 2009. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Wang A, Xu Q, Sha R, Bao T, Xi X and Guo G: MicroRNA‑29a inhibits cell proliferation and arrests cell cycle by modulating p16 methylation in cervical cancer. Oncol Lett 21: 272, 2021.
APA
Wang, A., Xu, Q., Sha, R., Bao, T., Xi, X., & Guo, G. (2021). MicroRNA‑29a inhibits cell proliferation and arrests cell cycle by modulating p16 methylation in cervical cancer. Oncology Letters, 21, 272. https://doi.org/10.3892/ol.2021.12533
MLA
Wang, A., Xu, Q., Sha, R., Bao, T., Xi, X., Guo, G."MicroRNA‑29a inhibits cell proliferation and arrests cell cycle by modulating p16 methylation in cervical cancer". Oncology Letters 21.4 (2021): 272.
Chicago
Wang, A., Xu, Q., Sha, R., Bao, T., Xi, X., Guo, G."MicroRNA‑29a inhibits cell proliferation and arrests cell cycle by modulating p16 methylation in cervical cancer". Oncology Letters 21, no. 4 (2021): 272. https://doi.org/10.3892/ol.2021.12533
Copy and paste a formatted citation
x
Spandidos Publications style
Wang A, Xu Q, Sha R, Bao T, Xi X and Guo G: MicroRNA‑29a inhibits cell proliferation and arrests cell cycle by modulating p16 methylation in cervical cancer. Oncol Lett 21: 272, 2021.
APA
Wang, A., Xu, Q., Sha, R., Bao, T., Xi, X., & Guo, G. (2021). MicroRNA‑29a inhibits cell proliferation and arrests cell cycle by modulating p16 methylation in cervical cancer. Oncology Letters, 21, 272. https://doi.org/10.3892/ol.2021.12533
MLA
Wang, A., Xu, Q., Sha, R., Bao, T., Xi, X., Guo, G."MicroRNA‑29a inhibits cell proliferation and arrests cell cycle by modulating p16 methylation in cervical cancer". Oncology Letters 21.4 (2021): 272.
Chicago
Wang, A., Xu, Q., Sha, R., Bao, T., Xi, X., Guo, G."MicroRNA‑29a inhibits cell proliferation and arrests cell cycle by modulating p16 methylation in cervical cancer". Oncology Letters 21, no. 4 (2021): 272. https://doi.org/10.3892/ol.2021.12533
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team