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Inhibition of SUMO2/3 antagonizes isoflurane‑induced cancer‑promoting effect in hepatocellular carcinoma Hep3B cells

  • Authors:
    • Peng Wang
    • Na Xue
    • Chunyan Zhang
    • Shimin Shan
    • Zhongmin Jiang
    • Wenhan Wu
    • Xiaozhi Liu
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, The Fifth Central Hospital of Tianjin, Tianjin 300450, P.R. China, Tianjin Key Laboratory of Epigenetics for Organ Development in Preterm Infants, The Fifth Central Hospital of Tianjin, Tianjin 300450, P.R. China, Department of Pharmacy, Binhai New Area Hospital of Traditional Chinese Medicine, Tianjin 300450, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 274
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    Published online on: February 10, 2021
       https://doi.org/10.3892/ol.2021.12535
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Abstract

Surgery for patients with complicated liver cancer often results in a long exposure to anesthesia with an increase in side effects. Continued long‑term exposure to isoflurane may promote liver cancer progression. Small ubiquitin‑like modifier (SUMO) 2 and 3, also known as SUMO2/3, conjugates to substrate proteins when cells undergo acute stress. However, whether or not SUMO2/3 is involved in isoflurane‑mediated liver cancer progression is unknown. In the present study, hepatocellular carcinoma (HCC) cells were exposed to 2% isoflurane for 12 h, followed by 36 h of drug withdrawal, and the formation of SUMO2/3 conjugates and cancer behavioral characteristics were studied. The results demonstrated that the formation of SUMO2/3 conjugates was significantly increased following HCC cells being exposed to isoflurane for 0.5 h, and continued to increase for 48 h, even after the drug had been withdrawn. Furthermore, isoflurane‑exposed HCC cells exhibited increased proliferation and invasion activity during the subsequent observation period. SUMO specific protease 3 (SENP3), which inhibits the binding of SUMO2/3 to its target proteins, was overexpressed and it was discovered that isoflurane‑induced SUMOylation was significantly inhibited, and accordingly, the proliferation and invasion abilities of HCC cells were decreased to a certain extent. These findings indicated that SUMO2/3 is involved in the progression of HCC cells, at least in the Hep3B cell line, induced by the anesthetic isoflurane, and that inhibition of SUMO2/3 may antagonize the response. These results provided a novel target for decreasing the adverse reactions occurring in patients with HCC during anesthesia, particularly those who are exposed to isoflurane for long periods of time.
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Copy and paste a formatted citation
Spandidos Publications style
Wang P, Xue N, Zhang C, Shan S, Jiang Z, Wu W and Liu X: Inhibition of SUMO2/3 antagonizes isoflurane‑induced cancer‑promoting effect in hepatocellular carcinoma Hep3B cells. Oncol Lett 21: 274, 2021.
APA
Wang, P., Xue, N., Zhang, C., Shan, S., Jiang, Z., Wu, W., & Liu, X. (2021). Inhibition of SUMO2/3 antagonizes isoflurane‑induced cancer‑promoting effect in hepatocellular carcinoma Hep3B cells. Oncology Letters, 21, 274. https://doi.org/10.3892/ol.2021.12535
MLA
Wang, P., Xue, N., Zhang, C., Shan, S., Jiang, Z., Wu, W., Liu, X."Inhibition of SUMO2/3 antagonizes isoflurane‑induced cancer‑promoting effect in hepatocellular carcinoma Hep3B cells". Oncology Letters 21.4 (2021): 274.
Chicago
Wang, P., Xue, N., Zhang, C., Shan, S., Jiang, Z., Wu, W., Liu, X."Inhibition of SUMO2/3 antagonizes isoflurane‑induced cancer‑promoting effect in hepatocellular carcinoma Hep3B cells". Oncology Letters 21, no. 4 (2021): 274. https://doi.org/10.3892/ol.2021.12535
Copy and paste a formatted citation
x
Spandidos Publications style
Wang P, Xue N, Zhang C, Shan S, Jiang Z, Wu W and Liu X: Inhibition of SUMO2/3 antagonizes isoflurane‑induced cancer‑promoting effect in hepatocellular carcinoma Hep3B cells. Oncol Lett 21: 274, 2021.
APA
Wang, P., Xue, N., Zhang, C., Shan, S., Jiang, Z., Wu, W., & Liu, X. (2021). Inhibition of SUMO2/3 antagonizes isoflurane‑induced cancer‑promoting effect in hepatocellular carcinoma Hep3B cells. Oncology Letters, 21, 274. https://doi.org/10.3892/ol.2021.12535
MLA
Wang, P., Xue, N., Zhang, C., Shan, S., Jiang, Z., Wu, W., Liu, X."Inhibition of SUMO2/3 antagonizes isoflurane‑induced cancer‑promoting effect in hepatocellular carcinoma Hep3B cells". Oncology Letters 21.4 (2021): 274.
Chicago
Wang, P., Xue, N., Zhang, C., Shan, S., Jiang, Z., Wu, W., Liu, X."Inhibition of SUMO2/3 antagonizes isoflurane‑induced cancer‑promoting effect in hepatocellular carcinoma Hep3B cells". Oncology Letters 21, no. 4 (2021): 274. https://doi.org/10.3892/ol.2021.12535
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