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Triptolide inhibits human telomerase reverse transcriptase by downregulating translation factors SP1 and c‑Myc in Epstein‑Barr virus‑positive B lymphocytes

  • Authors:
    • Cong Long
    • Qiu-Bo Xu
    • Li Ding
    • Liu Yang
    • Wei Ji
    • Feng Gao
    • Yong Ji
  • View Affiliations / Copyright

    Affiliations: Clinical Laboratory, Jingjiang People's Hospital, Jingjiang, Jiangsu 214500, P.R. China, Department of General Surgery, Jingjiang People's Hospital, Jingjiang, Jiangsu 214500, P.R. China
    Copyright: © Long et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 280
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    Published online on: February 10, 2021
       https://doi.org/10.3892/ol.2021.12541
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Abstract

Epstein‑Barr virus (EBV) mainly causes infectious mononucleosis and is associated with several neoplasms, including Burkitt's lymphoma, nasopharyngeal carcinoma and lymphoproliferative disease. Human telomerase reverse transcriptase (hTERT) regulates enzymatic activity of telomerase and is closely associated with tumorigenesis and senescence evasion. Triptolide (TP) is a diterpenoid triepoxide, with a broad‑spectrum anticancer and immunosuppressive bioactivity profile. The present study investigated whether TP inhibited hTERT expression and suppressed its activity. The mRNA and protein levels of hTERT were examined by reverse transcription‑quantitative PCR and western blotting. The activity of hTERT promoter was determined by dual‑luciferase reporter assay. Cell Counting Kit‑8 assays were performed to analyze cell proliferation. The present study used EBV‑positive B lymphoma cells as a model system, and the results demonstrated that TP significantly decreased hTERT transcription and protein expression. Mechanistically, TP attenuated the hTERT promoter activity by downregulating the expression levels of specificityprotein 1 and c‑Myc transcription factors. Consistently, inhibition of hTERT via shRNA transfection efficiently enhanced the suppression of cell proliferation by TP. Furthermore, TP increased virus latent replication and promoted the lytic cycle of EBV in EBV‑positive B lymphoma cells, increasing the number of lytic cells and inhibiting the viability of tumor cells. Taken together, the results of the present study revealed a molecular mechanism of the pharmacological inhibition of tumor cell proliferation by TP, encouraging the translation of TP‑based therapeutics in EBV‑positive B lymphoma treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Long C, Xu Q, Ding L, Yang L, Ji W, Gao F and Ji Y: Triptolide inhibits human telomerase reverse transcriptase by downregulating translation factors SP1 and c‑Myc in Epstein‑Barr virus‑positive B lymphocytes. Oncol Lett 21: 280, 2021.
APA
Long, C., Xu, Q., Ding, L., Yang, L., Ji, W., Gao, F., & Ji, Y. (2021). Triptolide inhibits human telomerase reverse transcriptase by downregulating translation factors SP1 and c‑Myc in Epstein‑Barr virus‑positive B lymphocytes. Oncology Letters, 21, 280. https://doi.org/10.3892/ol.2021.12541
MLA
Long, C., Xu, Q., Ding, L., Yang, L., Ji, W., Gao, F., Ji, Y."Triptolide inhibits human telomerase reverse transcriptase by downregulating translation factors SP1 and c‑Myc in Epstein‑Barr virus‑positive B lymphocytes". Oncology Letters 21.4 (2021): 280.
Chicago
Long, C., Xu, Q., Ding, L., Yang, L., Ji, W., Gao, F., Ji, Y."Triptolide inhibits human telomerase reverse transcriptase by downregulating translation factors SP1 and c‑Myc in Epstein‑Barr virus‑positive B lymphocytes". Oncology Letters 21, no. 4 (2021): 280. https://doi.org/10.3892/ol.2021.12541
Copy and paste a formatted citation
x
Spandidos Publications style
Long C, Xu Q, Ding L, Yang L, Ji W, Gao F and Ji Y: Triptolide inhibits human telomerase reverse transcriptase by downregulating translation factors SP1 and c‑Myc in Epstein‑Barr virus‑positive B lymphocytes. Oncol Lett 21: 280, 2021.
APA
Long, C., Xu, Q., Ding, L., Yang, L., Ji, W., Gao, F., & Ji, Y. (2021). Triptolide inhibits human telomerase reverse transcriptase by downregulating translation factors SP1 and c‑Myc in Epstein‑Barr virus‑positive B lymphocytes. Oncology Letters, 21, 280. https://doi.org/10.3892/ol.2021.12541
MLA
Long, C., Xu, Q., Ding, L., Yang, L., Ji, W., Gao, F., Ji, Y."Triptolide inhibits human telomerase reverse transcriptase by downregulating translation factors SP1 and c‑Myc in Epstein‑Barr virus‑positive B lymphocytes". Oncology Letters 21.4 (2021): 280.
Chicago
Long, C., Xu, Q., Ding, L., Yang, L., Ji, W., Gao, F., Ji, Y."Triptolide inhibits human telomerase reverse transcriptase by downregulating translation factors SP1 and c‑Myc in Epstein‑Barr virus‑positive B lymphocytes". Oncology Letters 21, no. 4 (2021): 280. https://doi.org/10.3892/ol.2021.12541
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