Curcumol inhibits the viability and invasion of colorectal cancer cells via miR‑30a‑5p and Hippo signaling pathway

Yu,Dan; Liu,Haiping; Qin,Jianli; Huangfu,Mengjie; Guan,Xiao; Li,Xumei; Zhou,Luwei; Dou,Tong; Liu,Yisa; Wang,Lin; Fu,Minglei; Wang,Juan; Chen,Xu

doi:10.3892/ol.2021.12560

Curcumol inhibits the viability and invasion of colorectal cancer cells via miR‑30a‑5p and Hippo signaling pathway

Authors:
- Dan Yu
- Haiping Liu
- Jianli Qin
- Mengjie Huangfu
- Xiao Guan
- Xumei Li
- Luwei Zhou
- Tong Dou
- Yisa Liu
- Lin Wang
- Minglei Fu
- Juan Wang
- Xu Chen
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Affiliations: Department of Pharmacy, Guilin Medical University, Guilin, Guangxi Zhuang Autonomous Region 541001, P.R. China, Science and Technology Department, Guilin Medical University, Guilin, Guangxi Zhuang Autonomous Region 541199, P.R. China, Department of Pharmacology, Xiangya School of Medicine of Central South University, Changsha, Hunan 410008, P.R. China, Department of Dispensary, The Second Affiliated Hospital of Guilin Medical University, Guilin, Guangxi Zhuang Autonomous Region 541001, P.R. China
Published online on: February 17, 2021 https://doi.org/10.3892/ol.2021.12560
Article Number: 299
Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNA‑30a‑5p (miR‑30a‑5p), which functions as a tumor suppressor, has been reported to be downregulated in colorectal cancer (CRC) tissues and to be associated with cancer invasion. However, the detailed regulatory mechanism of curcumol in the malignant progression of CRC remains unknown. MTT, Transwell, scratch, western blotting and reverse transcription‑quantitative PCR assays were performed to examine how curcumol inhibited CRC cell viability, invasion and migration, and to detect the role of miR‑30a‑5p and curcumol in the invasion and Hippo signaling pathways of CRC cells. The present study revealed that miR‑30a‑5p expression was downregulated in human CRC tissues and cells. The results demonstrated that miR‑30a‑5p downregulation was accompanied by the inactivation of the Hippo signaling pathway, which was demonstrated to promote CRC cell viability, invasion and migration. Curcumol treatment was identified to increase miR‑30a‑5p expression and to activate the Hippo signaling pathway, which in turn inhibited the invasion and migration of CRC cells. Overexpression of miR‑30a‑5p enhanced the effects of curcumol on cell invasion and migration, and the Hippo signaling pathway in CRC cells. Furthermore, downregulation of miR‑30a‑5p reversed the effects of curcumol on cell invasion and migration, and the Hippo signaling pathway in CRC cells. These findings identified novel signaling pathways associated with miR‑30a‑5p and revealed the effects of curcumol on miR‑30a‑5p expression. Therefore, curcumol may serve as a potential therapeutic strategy to delay CRC progression.

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