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Nuclear factor‑κB inhibitor Bay11‑7082 inhibits gastric cancer cell proliferation by inhibiting Gli1 expression

  • Authors:
    • Yan Yan
    • Heya Qian
    • Ying Cao
    • Tao Zhu
  • View Affiliations

  • Published online on: February 17, 2021     https://doi.org/10.3892/ol.2021.12562
  • Article Number: 301
  • Copyright: © Yan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Dysregulated nuclear factor (NF)‑κB signaling pathway is involved in gastric carcinogenesis. The present study aimed to investigate the antitumor effects of the NF‑κB inhibitor, Bay11‑7082, on gastric cancer (GC) and elucidate its underlying molecular mechanisms. The MTT assay was performed to assess the effects of Bay11‑7082 on the proliferation of HGC27 and MKN45 gastric cancer cells. In addition, the Transwell and wound healing assays were performed to determine cell migration and invasion, respectively. Reverse transcription‑quantitative PCR and western blot analyses were performed to detect the mRNA and protein expression levels of the target genes. The results demonstrated that the half‑maximal inhibitory concentration (IC50) of Bay11‑7082 in HGC27 cells was 24.88, 6.72 and 4.23 nM at 24, 48 and 72 h, respectively. Furthermore, the IC50 of Bay11‑7082 in MKN45 cells was 29.11, 11.22 and 5.88 nM at 24, 48 and 72 h, respectively. Treatment with Bay11‑7082 significantly suppressed the cell migratory and invasive abilities compared with the control group. Notably, Bay11‑7082 suppressed GLI Family Zinc Finger 1 (Gli1) mRNA and protein expression levels. Taken together, the results of the present study demonstrated that Bay11‑7082 inhibited GC cell proliferation, at least in part through inhibition of Gli1.
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April-2021
Volume 21 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Yan Y, Qian H, Cao Y and Zhu T: Nuclear factor‑κB inhibitor Bay11‑7082 inhibits gastric cancer cell proliferation by inhibiting Gli1 expression. Oncol Lett 21: 301, 2021
APA
Yan, Y., Qian, H., Cao, Y., & Zhu, T. (2021). Nuclear factor‑κB inhibitor Bay11‑7082 inhibits gastric cancer cell proliferation by inhibiting Gli1 expression. Oncology Letters, 21, 301. https://doi.org/10.3892/ol.2021.12562
MLA
Yan, Y., Qian, H., Cao, Y., Zhu, T."Nuclear factor‑κB inhibitor Bay11‑7082 inhibits gastric cancer cell proliferation by inhibiting Gli1 expression". Oncology Letters 21.4 (2021): 301.
Chicago
Yan, Y., Qian, H., Cao, Y., Zhu, T."Nuclear factor‑κB inhibitor Bay11‑7082 inhibits gastric cancer cell proliferation by inhibiting Gli1 expression". Oncology Letters 21, no. 4 (2021): 301. https://doi.org/10.3892/ol.2021.12562