Effects of targeted Notch1 silencing on the biological processes of the T24 and 5637 cells <em>in vitro</em>

Zheng,Kewen; Han,Xiaomin; Su,Yan; Wang,Qinghai; Ma,Qiang; Zheng,Kesi

doi:10.3892/ol.2021.12566

Effects of targeted Notch1 silencing on the biological processes of the T24 and 5637 cells in vitro

Authors:
- Kewen Zheng
- Xiaomin Han
- Yan Su
- Qinghai Wang
- Qiang Ma
- Kesi Zheng
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Affiliations: Department of Urology, The First Affiliated Hospital of Wenzhou Medical University, The First Clinical College of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China, Blood Conservation Institute, School of Basic and Forensic Medicine, Baotou Medical College, Baotou, Inner Mongolia Autonomous Region 014040, P.R. China, Department of Kidney Transplantation, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China, Department of Thyroid and Breast Surgery, Wenzhou People's Hospital, The Third Clinical College of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China
Published online on: February 21, 2021 https://doi.org/10.3892/ol.2021.12566
Article Number: 305
Copyright: © Zheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to investigate the roles of Notch1 in the biological processes of bladder cancer cells (BCCs) in vitro. Short hairpin (sh)RNA targeting Notch1 was designed and constructed, and the T24 and 5637 BCCs were selected for transfection. The cells were classified into two groups: shRNA negative control (NC) and Notch1 shRNA. MTT and Transwell assays, and flow cytometry were performed to examine the changes in cell proliferation, invasiveness, and apoptosis, respectively. In addition, reverse transcription‑quantitative PCR and western blot analysis was used to detect the mRNA and protein expression levels of apoptosis‑related proteins (Bax, Bid and Bcl2) and epithelial‑mesenchymal transition factors (vimentin and E‑ and N‑cadherin). Compared with that in the shRNA NC group, the Notch1 shRNA group showed significantly decreased cell proliferation rate and invasiveness; increased apoptotic rate; elevated mRNA expression levels of Bad, Bid and E‑cadherin; and reduced mRNA expression levels of Bcl2, N‑cadherin and vimentin. The trends for protein expression levels were the same as those for mRNA levels. Notch1 silencing inhibited invasion and promoted apoptosis of BCCs.

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