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Article

Expression of canonical transient receptor potential channels in U-2 OS and MNNG-HOS osteosarcoma cell lines

  • Authors:
    • Florian Lässig
    • Anja Klann
    • Sander Bekeschus
    • Uwe Lendeckel
    • Carmen Wolke
  • View Affiliations / Copyright

    Affiliations: Institute of Medical Biochemistry and Molecular Biology, University Medicine Greifswald, D-17475 Greifswald, Germany, Institute of Forensic Medicine, University Medicine Greifswald, D-17489, Greifswald, Germany, Zentrum für Innovationskompetenz (ZIK) plasmatis, Leibniz Institute for Plasma Science and Technology (INP), D-17489 Greifswald, Germany
  • Article Number: 307
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    Published online on: February 21, 2021
       https://doi.org/10.3892/ol.2021.12568
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Abstract

In U-2 OS and MNNG-HOS osteosarcoma cells, small interfering RNA-mediated knockdown of the angiotensin-(1-7) receptor, Mas, increases cell proliferation. Whether alterations in canonical transient receptor potential channels (TRPC) expression contribute to this effect is not clear. In the present study, a basic description of TRPC subtype expression in osteosarcoma cell lines was provided. The pharmacological modulators of the angiotensin-(1-7) receptor, Mas, AVE0991 (agonist), or D-Ala7-Ang-(1-7) (antagonist) were applied to elucidate a possible role of Mas in the regulation of TRPC mRNA levels. The contribution of other G-protein coupled receptors (GPCR) or receptor tyrosine kinases to TRCP expression was studied by applying the selective pharmacological blockers of either PI3 kinase or MEK/Erk1/2 signaling, Ly294002 and PD98059. AVE0991 and D-Ala7-Ang-(1-7) exhibited no or marginal effects on TRPC mRNA expression. Ly294002 provoked a 9.6- and 5.9-fold increase in the amounts of TRPC5 mRNA in MNNG-HOS and U-2 OS cells, respectively. Additionally, Ly294002 increased TRPC6 mRNA levels; however, it had no effect on TRPCs 1, 3 and 4. Administration of PD98059 increased the amounts of TRPC6 and TRPC4 ~2-fold. In conclusion, the present study demonstrated that Mas-dependent alterations in osteosarcoma cell line proliferation were not mediated by any changes in TRPC subtype gene expression. The data shows in principle, and consistent with the literature, that the signaling pathways examined can regulate the expression of TRPCs at the mRNA level. Therefore, direct and signaling pathway-specific pharmacological targeting of TRPC subtypes may represent an option for improving the treatment of osteosarcoma.
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Copy and paste a formatted citation
Spandidos Publications style
Lässig F, Klann A, Bekeschus S, Lendeckel U and Wolke C: Expression of canonical transient receptor potential channels in U-2 OS and MNNG-HOS osteosarcoma cell lines. Oncol Lett 21: 307, 2021.
APA
Lässig, F., Klann, A., Bekeschus, S., Lendeckel, U., & Wolke, C. (2021). Expression of canonical transient receptor potential channels in U-2 OS and MNNG-HOS osteosarcoma cell lines. Oncology Letters, 21, 307. https://doi.org/10.3892/ol.2021.12568
MLA
Lässig, F., Klann, A., Bekeschus, S., Lendeckel, U., Wolke, C."Expression of canonical transient receptor potential channels in U-2 OS and MNNG-HOS osteosarcoma cell lines". Oncology Letters 21.4 (2021): 307.
Chicago
Lässig, F., Klann, A., Bekeschus, S., Lendeckel, U., Wolke, C."Expression of canonical transient receptor potential channels in U-2 OS and MNNG-HOS osteosarcoma cell lines". Oncology Letters 21, no. 4 (2021): 307. https://doi.org/10.3892/ol.2021.12568
Copy and paste a formatted citation
x
Spandidos Publications style
Lässig F, Klann A, Bekeschus S, Lendeckel U and Wolke C: Expression of canonical transient receptor potential channels in U-2 OS and MNNG-HOS osteosarcoma cell lines. Oncol Lett 21: 307, 2021.
APA
Lässig, F., Klann, A., Bekeschus, S., Lendeckel, U., & Wolke, C. (2021). Expression of canonical transient receptor potential channels in U-2 OS and MNNG-HOS osteosarcoma cell lines. Oncology Letters, 21, 307. https://doi.org/10.3892/ol.2021.12568
MLA
Lässig, F., Klann, A., Bekeschus, S., Lendeckel, U., Wolke, C."Expression of canonical transient receptor potential channels in U-2 OS and MNNG-HOS osteosarcoma cell lines". Oncology Letters 21.4 (2021): 307.
Chicago
Lässig, F., Klann, A., Bekeschus, S., Lendeckel, U., Wolke, C."Expression of canonical transient receptor potential channels in U-2 OS and MNNG-HOS osteosarcoma cell lines". Oncology Letters 21, no. 4 (2021): 307. https://doi.org/10.3892/ol.2021.12568
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