Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
May-2021 Volume 21 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
May-2021 Volume 21 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

MicroRNA‑26a inhibits cell proliferation and invasion by targeting FAM98A in breast cancer

  • Authors:
    • Tan Liu
    • Ziming Wang
    • Menghao Dong
    • Jingjing Wei
    • Yueyin Pan
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, Provincial Hospital Affiliated to Anhui Medical University, Hefei, Anhui 230032, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 367
    |
    Published online on: March 10, 2021
       https://doi.org/10.3892/ol.2021.12628
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

MicroRNAs (miRNAs/miRs) play key roles in cancer progression. Extensive research has revealed that miR‑26a is abnormally expressed and functions as a tumor suppressor in numerous types of cancer. Thus, the present study was undertaken to investigate the regulatory role and potential mechanism of action of miR‑26a in breast cancer. Furthermore, the present study aimed to examine the alterations in miR‑26a expression and its effects on human breast cancer cells. Reverse transcription‑quantitative PCR was conducted to assess the differences in miR‑26a expression between human breast cancer and normal breast specimens. A Cell Counting Kit‑8 assay and cloning experiments were used to detect cell proliferation and clone formation. Wound healing and Transwell assays were performed to examine cell migration and invasion. A luciferase activity experiment was utilized to validate the association between miR‑26a and family with sequence similarity 98 member A (FAM98A). Western blotting was conducted to detect the protein expression levels of FAM98A, sonic hedgehog signaling molecule (SHH), smoothened, frizzled class receptor (SMO) and GLI family zinc finger 1 (GLI1). The results indicated that miR‑26a expression was decreased in breast carcinoma tissues and cell lines. Moreover, overexpression of miR‑26a significantly suppressed cell proliferation, clone formation ability and metastasis, and it sensitized breast cancer cells to docetaxel. It was demonstrated that miR‑26a directly targeted FAM98A, and that FAM98A, SHH, SMO and GLI1 expression levels were decreased in cells transfected with miR‑26a mimics. Collectively, the results of the present study suggested that miR‑26a negatively regulated the expression of FAM98A, indicating that it may play a key role in the suppression of breast carcinogenesis.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Siegel RL, Miller KD and Jemal A: Cancer statistics, 2019. CA Cancer J Clin. 69:7–34. 2019. View Article : Google Scholar : PubMed/NCBI

2 

Baltzer PAT, Kapetas P, Marino MA and Clauser P: New diagnostic tools for breast cancer. Memo. 10:175–180. 2017. View Article : Google Scholar : PubMed/NCBI

3 

Gomez-Miragaya J, Moran S, Calleja-Cervantes ME, Collado-Sole A, Pare L, Gomez A, Serra V, Dobrolecki LE, Lewis MT, Diaz-Lagares A, et al: The altered transcriptome and DNA methylation profiles of docetaxel resistance in breast cancer PDX models. Mol Cancer Res. 17:2063–2076. 2019. View Article : Google Scholar : PubMed/NCBI

4 

Huang P, Li F, Li L, You Y, Luo S, Dong Z, Gao Q, Wu S, Brunner N and Stenvang J: lncRNA profile study reveals the mRNAs and lncRNAs associated with docetaxel resistance in breast cancer cells. Sci Rep. 8:179702018. View Article : Google Scholar : PubMed/NCBI

5 

Fan L, Strasser-Weippl K, Li JJ, St LJ, Finkelstein DM, Yu KD, Chen WQ, Shao ZM and Goss PE: Breast cancer in China. Lancet Oncol. 15:e279–e289. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Rupaimoole R and Slack FJ: MicroRNA therapeutics: Towards a new era for the management of cancer and other diseases. Nat Rev Drug Discov. 16:203–222. 2017. View Article : Google Scholar : PubMed/NCBI

7 

Guo H, Ingolia NT, Weissman JS and Bartel DP: Mammalian microRNAs predominantly act to decrease target mRNA levels. Nature. 466:835–840. 2010. View Article : Google Scholar : PubMed/NCBI

8 

Jeffries J, Zhou W, Hsu AY and Deng Q: miRNA-223 at the crossroads of inflammation and cancer. Cancer Lett. 451:136–141. 2019. View Article : Google Scholar : PubMed/NCBI

9 

Si W, Shen J, Zheng H and Fan W: The role and mechanisms of action of microRNAs in cancer drug resistance. Clin Epigenetics. 11:252019. View Article : Google Scholar : PubMed/NCBI

10 

He XH, Zhu W, Yuan P, Jiang S, Li D, Zhang HW and Liu MF: miR-155 downregulates ErbB2 and suppresses ErbB2-induced malignant transformation of breast epithelial cells. Oncogene. 35:6015–6025. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Jafri MA, Al-Qahtani MH and Shay JW: Role of miRNAs in human cancer metastasis: Implications for therapeutic intervention. Semin Cancer Biol. 44:117–131. 2017. View Article : Google Scholar : PubMed/NCBI

12 

Chen J, Zhang K, Xu Y, Gao Y, Li C, Wang R and Chen L: The role of microRNA-26a in human cancer progression and clinical application. Tumour Biol. 37:7095–7108. 2016. View Article : Google Scholar : PubMed/NCBI

13 

Huang ZM, Ge HF, Yang CC, Cai Y, Chen Z, Tian WZ and Tao JL: MicroRNA-26a-5p inhibits breast cancer cell growth by suppressing RNF6 expression. Kaohsiung J Med Sci. 35:467–473. 2019.PubMed/NCBI

14 

Akter KA, Mansour MA, Hyodo T, Ito S, Hamaguchi M and Senga T: FAM98A is a novel substrate of PRMT1 required for tumor cell migration, invasion, and colony formation. Tumour Biol. 37:4531–4539. 2016. View Article : Google Scholar : PubMed/NCBI

15 

Akter KA, Mansour MA, Hyodo T and Senga T: FAM98A associates with DDX1-C14orf166-FAM98B in a novel complex involved in colorectal cancer progression. Int J Biochem Cell Biol. 84:1–13. 2017. View Article : Google Scholar : PubMed/NCBI

16 

Li Z, Li N, Sun X and Wang J: FAM98A promotes cancer progression in endometrial carcinoma. Mol Cell Biochem. 459:131–139. 2019. View Article : Google Scholar : PubMed/NCBI

17 

Zheng R, Liu Q, Wang T, Wang L and Zhang Y: FAM98A promotes proliferation of non-small cell lung cancer cells via the P38-ATF2 signaling pathway. Cancer Manag Res. 10:2269–2278. 2018. View Article : Google Scholar : PubMed/NCBI

18 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

19 

Katoh M: Genomic testing, tumor microenvironment and targeted therapy of Hedgehog-related human cancers. Clin Sci (Lond). 133:953–970. 2019. View Article : Google Scholar : PubMed/NCBI

20 

Riaz SK, Khan JS, Shah STA, Wang F, Ye L, Jiang WG and Malik MFA: Involvement of hedgehog pathway in early onset, aggressive molecular subtypes and metastatic potential of breast cancer. Cell Commun Signal. 16:32018. View Article : Google Scholar : PubMed/NCBI

21 

Ma L: MicroRNA and Metastasis. Adv Cancer Res. 132:165–207. 2016. View Article : Google Scholar : PubMed/NCBI

22 

Braicu C, Raduly L, Morar-Bolba G, Cojocneanu R, Jurj A, Pop LA, Pileczki V, Ciocan C, Moldovan A, Irimie A, et al: Aberrant miRNAs expressed in HER-2 negative breast cancers patient. J Exp Clin Cancer Res. 37:2572018. View Article : Google Scholar : PubMed/NCBI

23 

Fridrichova I and Zmetakova I: MicroRNAs contribute to breast cancer invasiveness. Cells. 8:13612019. View Article : Google Scholar

24 

Biswas S: MicroRNAs as therapeutic agents: The future of the battle against cancer. Curr Top Med Chem. 18:2544–2554. 2018. View Article : Google Scholar : PubMed/NCBI

25 

Yang C, Tabatabaei SN, Ruan X and Hardy P: The dual regulatory role of MiR-181a in breast cancer. Cell Physiol Biochem. 44:843–856. 2017. View Article : Google Scholar : PubMed/NCBI

26 

Li Y, Wang P, Wu LL, Yan J, Pang XY and Liu SJ: miR-26a-5p inhibit gastric cancer cell proliferation and invasion through mediated Wnt5a. Onco Targets Ther. 13:2537–2550. 2020. View Article : Google Scholar : PubMed/NCBI

27 

Ma Y, Deng F, Li P, Chen G, Tao Y and Wang H: The tumor suppressive miR-26a regulation of FBXO11 inhibits proliferation, migration and invasion of hepatocellular carcinoma cells. Biomed Pharmacother. 101:648–655. 2018. View Article : Google Scholar : PubMed/NCBI

28 

Guo K, Zheng S, Xu Y, Xu A, Chen B and Wen Y: Loss of miR-26a-5p promotes proliferation, migration, and invasion in prostate cancer through negatively regulating SERBP1. Tumour Biol. 37:12843–12854. 2016. View Article : Google Scholar : PubMed/NCBI

29 

Wu F, Zhang Y, Sun B, McMahon AP and Wang Y: Hedgehog signaling: From basic biology to cancer therapy. Cell Chem Biol. 24:252–280. 2017. View Article : Google Scholar : PubMed/NCBI

30 

Hanna A and Shevde LA: Hedgehog signaling: Modulation of cancer properies and tumor mircroenvironment. Mol Cancer. 15:242016. View Article : Google Scholar : PubMed/NCBI

31 

Abe Y and Tanaka N: Fine-Tuning of GLI activity through arginine methylation: Its mechanisms and function. Cells. 9:19732020. View Article : Google Scholar

32 

Wang Y, Hsu JM, Kang Y, Wei Y, Lee PC, Chang SJ, Hsu YH, Hsu JL, Wang HL, Chang WC, et al: Oncogenic functions of gli1 in pancreatic adenocarcinoma are supported by its PRMT1-Mediated methylation. Cancer Res. 76:7049–7058. 2016. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Liu T, Wang Z, Dong M, Wei J and Pan Y: MicroRNA‑26a inhibits cell proliferation and invasion by targeting FAM98A in breast cancer. Oncol Lett 21: 367, 2021.
APA
Liu, T., Wang, Z., Dong, M., Wei, J., & Pan, Y. (2021). MicroRNA‑26a inhibits cell proliferation and invasion by targeting FAM98A in breast cancer. Oncology Letters, 21, 367. https://doi.org/10.3892/ol.2021.12628
MLA
Liu, T., Wang, Z., Dong, M., Wei, J., Pan, Y."MicroRNA‑26a inhibits cell proliferation and invasion by targeting FAM98A in breast cancer". Oncology Letters 21.5 (2021): 367.
Chicago
Liu, T., Wang, Z., Dong, M., Wei, J., Pan, Y."MicroRNA‑26a inhibits cell proliferation and invasion by targeting FAM98A in breast cancer". Oncology Letters 21, no. 5 (2021): 367. https://doi.org/10.3892/ol.2021.12628
Copy and paste a formatted citation
x
Spandidos Publications style
Liu T, Wang Z, Dong M, Wei J and Pan Y: MicroRNA‑26a inhibits cell proliferation and invasion by targeting FAM98A in breast cancer. Oncol Lett 21: 367, 2021.
APA
Liu, T., Wang, Z., Dong, M., Wei, J., & Pan, Y. (2021). MicroRNA‑26a inhibits cell proliferation and invasion by targeting FAM98A in breast cancer. Oncology Letters, 21, 367. https://doi.org/10.3892/ol.2021.12628
MLA
Liu, T., Wang, Z., Dong, M., Wei, J., Pan, Y."MicroRNA‑26a inhibits cell proliferation and invasion by targeting FAM98A in breast cancer". Oncology Letters 21.5 (2021): 367.
Chicago
Liu, T., Wang, Z., Dong, M., Wei, J., Pan, Y."MicroRNA‑26a inhibits cell proliferation and invasion by targeting FAM98A in breast cancer". Oncology Letters 21, no. 5 (2021): 367. https://doi.org/10.3892/ol.2021.12628
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team