Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
May-2021 Volume 21 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
May-2021 Volume 21 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Downregulation of long non‑coding RNA MR4435‑2HG suppresses breast cancer progression via the Wnt/β‑catenin signaling pathway

  • Authors:
    • Deqin Chen
    • Pengting Tang
    • Yike Wang
    • Fang Wan
    • Jingpei Long
    • Jun Zhou
    • Ming Zhuang
    • Xin Chen
  • View Affiliations / Copyright

    Affiliations: Department of Surgery, The Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310006, P.R. China, Department of Surgery, Ninghai Maternity and Child Care Hospital, Ningbo, Zhejiang 315600, P.R. China, Department of Breast Surgery, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200092, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 373
    |
    Published online on: March 13, 2021
       https://doi.org/10.3892/ol.2021.12634
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Extensive research has contributed to the current understanding of the critical roles played by long non‑coding RNAs in various types of cancer. The present study aimed to investigate the function and mechanism of the long non‑coding RNA, MIR4435‑2HG (also termed LINC00978), in breast cancer growth and metastasis. Using Gene Expression Profiling Interactive Analysis, an online web tool, it was revealed that MIR4435‑2HG was upregulated in breast cancer tissue, and its high expression was associated with poor prognosis based on The Cancer Genome Atlas database. MIR4435‑2HG knockdown increased cell apoptosis but decreased cell proliferation, migration and invasion. MIR4435‑2HG knockdown increased pro‑apoptotic protein expression but decreased anti‑apoptotic protein expression. In addition, MIR4435‑2HG knockdown leads to dysregulation of epithelial‑to‑mesenchymal transition‑associated genes. Furthermore, knockdown of MIR4435‑2HG results in inactivation of the Wnt/β‑catenin signaling pathway. The results of the present study demonstrate the tumor‑promoting role of MIR4435‑2HG in breast cancer progression.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Ferlay J, Shin HR, Bray F, Forman D, Mathers C and Parkin DM: Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int J Cancer. 127:2893–2917. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J and Jemal A: Global cancer statistics, 2012. CA Cancer J Clin. 65:87–108. 2015. View Article : Google Scholar : PubMed/NCBI

3 

Ribeiro Pereira ACP, Koifman RJ and Bergmann A: Incidence and risk factors of lymphedema after breast cancer treatment: 10 years of follow-up. Breast. 36:67–73. 2017. View Article : Google Scholar : PubMed/NCBI

4 

Lemler DJ, Lynch ML, Tesfay L, Deng Z, Paul BT, Wang X, Hegde P, Manz DH, Torti SV and Torti FM: DCYTB is a predictor of outcome in breast cancer that functions via iron-independent mechanisms. Breast Cancer Res. 19:252017. View Article : Google Scholar : PubMed/NCBI

5 

Cuzick J, DeCensi A, Arun B, Brown PH, Castiglione M, Dunn B, Forbes JF, Glaus A, Howell A, von Minckwitz G, et al: Preventive therapy for breast cancer: A consensus statement. Lancet Oncol. 12:496–503. 2011. View Article : Google Scholar : PubMed/NCBI

6 

Weigelt B, Peterse JL and van't Veer LJ: Breast cancer metastasis: Markers and models. Nat Rev Cancer. 5:591–602. 2005. View Article : Google Scholar : PubMed/NCBI

7 

Rinn JL and Chang HY: Genome regulation by long noncoding RNAs. Annu Rev Biochem. 81:145–166. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Hu YP, Jin YP, Wu XS, Yang Y, Li YS, Li HF, Xiang SS, Song XL, Jiang L, Zhang YJ, et al: LncRNA-HGBC stabilized by HuR promotes gallbladder cancer progression by regulating miR-502-3p/SET/AKT axis. Mol Cancer. 18:1672019. View Article : Google Scholar : PubMed/NCBI

9 

Lin C and Yang L: Long noncoding RNA in cancer: Wiring signaling circuitry. Trends Cell Biol. 28:287–301. 2018. View Article : Google Scholar : PubMed/NCBI

10 

Wang Z, Wang P, Cao L, Li F, Duan S, Yuan G, Xiao L, Guo L, Yin H, Xie D, et al: Long intergenic non-coding RNA 01121 promotes breast cancer cell proliferation, migration, and invasion via the miR-150-5p/HMGA2 axis. Cancer Manag Res. 11:10859–10870. 2019. View Article : Google Scholar : PubMed/NCBI

11 

Guo S, Jian L, Tao K, Chen C, Yu H and Liu S: Novel breast-specific long non-coding RNA LINC00993 acts as a tumor suppressor in triple-negative breast cancer. Front Oncol. 9:13252019. View Article : Google Scholar : PubMed/NCBI

12 

Hu Q, Li C, Wang S, Li Y, Wen B, Zhang Y, Liang K, Yao J, Ye Y, Hsiao H, et al: LncRNAs-directed PTEN enzymatic switch governs epithelial-mesenchymal transition. Cell Res. 29:286–304. 2019. View Article : Google Scholar : PubMed/NCBI

13 

Jin C, Yan B, Lu Q, Lin Y and Ma L: Reciprocal regulation of Hsa-miR-1 and long noncoding RNA MALAT1 promotes triple-negative breast cancer development. Tumour Biol. 37:7383–7394. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Xu X, Gu J, Ding X, Ge G, Zang X, Ji R, Shao M, Mao Z, Zhang Y, Zhang J, et al: LINC00978 promotes the progression of hepatocellular carcinoma by regulating EZH2-mediated silencing of p21 and E-cadherin expression. Cell Death Dis. 10:7522019. View Article : Google Scholar : PubMed/NCBI

15 

Qian H, Chen L, Huang J, Wang X, Ma S, Cui F, Luo L, Ling L, Luo K and Zheng G: The lncRNA MIR4435-2HG promotes lung cancer progression by activating β-catenin signalling. J Mol Med (Berl). 96:753–764. 2018. View Article : Google Scholar : PubMed/NCBI

16 

Li X, Ren Y and Zuo T: Long noncoding RNA LINC00978 promotes cell proliferation and invasion in nonsmall cell lung cancer by inhibiting miR-6754-5p. Mol Med Rep. 18:4725–4732. 2018.PubMed/NCBI

17 

Wang H, Wu M, Lu Y, He K, Cai X, Yu X, Lu J and Teng L: LncRNA MIR4435-2HG targets desmoplakin and promotes growth and metastasis of gastric cancer by activating Wnt/β-catenin signaling. Aging (Albany NY). 11:6657–6673. 2019. View Article : Google Scholar : PubMed/NCBI

18 

Luo P, Wu SG, Ji KB, Yuan X, Li HM, Chen JP, Tian YF, Qiu Y and Zhong XM: LncRNA MIR4435-2HG mediates cisplatin resistance in HCT116 cells by regulating Nrf2 and HO-1. PLoS One. 15:e02230352020. View Article : Google Scholar : PubMed/NCBI

19 

Zhu LJ, Wang AH, Gao M, Duan XY and Li ZH: LncRNA MIR4435-2HG triggers ovarian cancer progression by regulating miR-128-3p/CKD14 axis. Cancer Cell Int. 20:1452020. View Article : Google Scholar : PubMed/NCBI

20 

Deng LL, Chi YY, Liu L, Huang NS, Wang L and Wu J: LINC00978 predicts poor prognosis in breast cancer patients. Sci Rep. 6:379362016. View Article : Google Scholar : PubMed/NCBI

21 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

22 

Tang Z, Li C, Kang B, Gao G, Li C and Zhang Z: GEPIA: A web server for cancer and normal gene expression profiling and interactive analyses. Nucleic Acids Res. 45:W98–W102. 2017. View Article : Google Scholar : PubMed/NCBI

23 

Pohl SG, Brook N, Agostino M, Arfuso F, Kumar AP and Dharmarajan A: Wnt signaling in triple-negative breast cancer. Oncogenesis. 6:e3102017. View Article : Google Scholar : PubMed/NCBI

24 

Liu S, Qiao Z, Ma Q, Liu X and Ma X: LncRNA CYTOR and MIR4435-2HG in ovarian cancer and its relationship with clinicopathological features. Panminerva Med. 2019.(Epub ahead of print).

25 

Zhang H, Meng H, Huang X, Tong W, Liang X, Li J, Zhang C and Chen M: lncRNA MIR4435-2HG promotes cancer cell migration and invasion in prostate carcinoma by upregulating TGF-β1. Oncol Lett. 18:4016–4021. 2019.PubMed/NCBI

26 

Hengartner MO: The biochemistry of apoptosis. Nature. 407:770–776. 2000. View Article : Google Scholar : PubMed/NCBI

27 

McIlwain DR, Berger T and Mak TW: Caspase functions in cell death and disease. Cold Spring Harb Perspect Biol. 5:a0086562013. View Article : Google Scholar : PubMed/NCBI

28 

Degterev A, Boyce M and Yuan J: A decade of caspases. Oncogene. 22:8543–8567. 2003. View Article : Google Scholar : PubMed/NCBI

29 

Suliman A, Lam A, Datta R and Srivastava RK: Intracellular mechanisms of TRAIL: Apoptosis through mitochondrial-dependent and -independent pathways. Oncogene. 20:2122–2133. 2001. View Article : Google Scholar : PubMed/NCBI

30 

Lamouille S, Xu J and Derynck R: Molecular mechanisms of epithelial-mesenchymal transition. Nat Rev Mol Cell Biol. 15:178–196. 2014. View Article : Google Scholar : PubMed/NCBI

31 

Thiery JP, Acloque H, Huang RY and Nieto MA: Epithelial-mesenchymal transitions in development and disease. Cell. 139:871–890. 2009. View Article : Google Scholar : PubMed/NCBI

32 

Kalluri R and Weinberg RA: The basics of epithelial-mesenchymal transition. J Clin Invest. 119:1420–1428. 2009. View Article : Google Scholar : PubMed/NCBI

33 

Wang YP, Guo PT, Zhu Z, Zhang H, Xu Y, Chen YZ, Liu F and Ma SP: Pleomorphic adenoma gene like-2 induces epithelial-mesenchymal transition via Wnt/β-catenin signaling pathway in human colorectal adenocarcinoma. Oncol Rep. 37:1961–1970. 2017. View Article : Google Scholar : PubMed/NCBI

34 

Zhang P, Zhao X, Zhang W, He A, Lei B, Zhang W and Chen Y: Leukemia-associated gene MLAA-34 reduces arsenic trioxide-induced apoptosis in HeLa cells via activation of the Wnt/β-catenin signaling pathway. PLoS One. 12:e01868682017. View Article : Google Scholar : PubMed/NCBI

35 

Shao HM, Ma JH, Guo TH and Hu RR: Triptolide induces apoptosis of breast cancer cells via a mechanism associated with the Wnt/β-catenin signaling pathway. Exp Ther Med. 8:505–508. 2014. View Article : Google Scholar : PubMed/NCBI

36 

Knobloch J, Schmitz I, Götz K, Schulze-Osthoff K and Rüther U: Thalidomide induces limb anomalies by PTEN stabilization, akt suppression, and stimulation of caspase-dependent cell death. Mol Cell Biol. 28:529–538. 2008. View Article : Google Scholar : PubMed/NCBI

37 

Yang M, He X, Huang X, Wang J, He Y and Wei L: LncRNA MIR4435-2HG-mediated upregulation of TGF-beta1 promotes migration and proliferation of nonsmall cell lung cancer cells. Environ Toxicol. 35:582–590. 2020. View Article : Google Scholar : PubMed/NCBI

38 

Kong Q, Liang C, Jin Y, Pan Y, Tong D, Kong Q and Zhou J: The lncRNA MIR4435-2HG is upregulated in hepatocellular carcinoma and promotes cancer cell proliferation by upregulating miRNA-487a. Cell Mol Biol Lett. 24:262019. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Chen D, Tang P, Wang Y, Wan F, Long J, Zhou J, Zhuang M and Chen X: Downregulation of long non‑coding RNA MR4435‑2HG suppresses breast cancer progression via the Wnt/β‑catenin signaling pathway. Oncol Lett 21: 373, 2021.
APA
Chen, D., Tang, P., Wang, Y., Wan, F., Long, J., Zhou, J. ... Chen, X. (2021). Downregulation of long non‑coding RNA MR4435‑2HG suppresses breast cancer progression via the Wnt/β‑catenin signaling pathway. Oncology Letters, 21, 373. https://doi.org/10.3892/ol.2021.12634
MLA
Chen, D., Tang, P., Wang, Y., Wan, F., Long, J., Zhou, J., Zhuang, M., Chen, X."Downregulation of long non‑coding RNA MR4435‑2HG suppresses breast cancer progression via the Wnt/β‑catenin signaling pathway". Oncology Letters 21.5 (2021): 373.
Chicago
Chen, D., Tang, P., Wang, Y., Wan, F., Long, J., Zhou, J., Zhuang, M., Chen, X."Downregulation of long non‑coding RNA MR4435‑2HG suppresses breast cancer progression via the Wnt/β‑catenin signaling pathway". Oncology Letters 21, no. 5 (2021): 373. https://doi.org/10.3892/ol.2021.12634
Copy and paste a formatted citation
x
Spandidos Publications style
Chen D, Tang P, Wang Y, Wan F, Long J, Zhou J, Zhuang M and Chen X: Downregulation of long non‑coding RNA MR4435‑2HG suppresses breast cancer progression via the Wnt/β‑catenin signaling pathway. Oncol Lett 21: 373, 2021.
APA
Chen, D., Tang, P., Wang, Y., Wan, F., Long, J., Zhou, J. ... Chen, X. (2021). Downregulation of long non‑coding RNA MR4435‑2HG suppresses breast cancer progression via the Wnt/β‑catenin signaling pathway. Oncology Letters, 21, 373. https://doi.org/10.3892/ol.2021.12634
MLA
Chen, D., Tang, P., Wang, Y., Wan, F., Long, J., Zhou, J., Zhuang, M., Chen, X."Downregulation of long non‑coding RNA MR4435‑2HG suppresses breast cancer progression via the Wnt/β‑catenin signaling pathway". Oncology Letters 21.5 (2021): 373.
Chicago
Chen, D., Tang, P., Wang, Y., Wan, F., Long, J., Zhou, J., Zhuang, M., Chen, X."Downregulation of long non‑coding RNA MR4435‑2HG suppresses breast cancer progression via the Wnt/β‑catenin signaling pathway". Oncology Letters 21, no. 5 (2021): 373. https://doi.org/10.3892/ol.2021.12634
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team