Open Access

MicroRNA‑223‑5p suppresses the progression of nasopharyngeal carcinoma by targeting DCLK1

  • Authors:
    • Zhixuan Zhang
    • Haifeng Li
    • Jianqiang You
    • Haixiang Xue
    • Xiaoye Tan
    • Changjiang Chao
  • View Affiliations

  • Published online on: March 18, 2021     https://doi.org/10.3892/ol.2021.12657
  • Article Number: 396
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The aim of the present study was to investigate the function of microRNA (miR)‑223‑5p in the malignant biological behavior of nasopharyngeal carcinoma (NPC) and elucidate the underlying molecular mechanism. The expression levels of miR‑223‑5p and doublecortin‑like kinase 1 (DCLK1) were detected via reverse transcription‑quantitative PCR analysis. Cell viability was evaluated using Cell Counting Kit‑8 assay. Cell migration and invasion were measured via Transwell assays, while a luciferase reporter assay was conducted to identify the interaction between miR‑223‑5p and DCLK1. The results demonstrated that miR‑223‑5p expression was significantly downregulated, whereas DCLK1 expression was significantly upregulated in NPC tissues and cells. Moreover, both miR‑223‑5p overexpression and DCLK1 silencing markedly suppressed the progression of NPC. It was also observed that miR‑223‑5p directly targeted DCLK1 and decreased its expression. Furthermore, it was suggested that DCLK1 overexpression may partially reverse the suppressive effects of miR‑223‑5p on the progression of NPC. Collectively, the results of the present study indicated that miR‑223‑5p may suppress NPC progression by targeting DCLK1, thereby indicating a novel potential approach to the diagnosis and treatment of NPC.
View Figures
View References

Related Articles

Journal Cover

May-2021
Volume 21 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Z, Li H, You J, Xue H, Tan X and Chao C: MicroRNA‑223‑5p suppresses the progression of nasopharyngeal carcinoma by targeting DCLK1. Oncol Lett 21: 396, 2021
APA
Zhang, Z., Li, H., You, J., Xue, H., Tan, X., & Chao, C. (2021). MicroRNA‑223‑5p suppresses the progression of nasopharyngeal carcinoma by targeting DCLK1. Oncology Letters, 21, 396. https://doi.org/10.3892/ol.2021.12657
MLA
Zhang, Z., Li, H., You, J., Xue, H., Tan, X., Chao, C."MicroRNA‑223‑5p suppresses the progression of nasopharyngeal carcinoma by targeting DCLK1". Oncology Letters 21.5 (2021): 396.
Chicago
Zhang, Z., Li, H., You, J., Xue, H., Tan, X., Chao, C."MicroRNA‑223‑5p suppresses the progression of nasopharyngeal carcinoma by targeting DCLK1". Oncology Letters 21, no. 5 (2021): 396. https://doi.org/10.3892/ol.2021.12657