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miR‑20a‑5p inhibits endometrial cancer progression by targeting janus kinase 1

  • Authors:
    • Ying He
    • Hui Ma
    • Jing Wang
    • Yanhua Kang
    • Qianlong Xue
  • View Affiliations / Copyright

    Affiliations: Department of Gynaecology, The First Affiliated Hospital of Hebei North University, Zhangjiakou, Hebei 075000, P.R. China, Emergency Department, The First Affiliated Hospital of Hebei North University, Zhangjiakou, Hebei 075000, P.R. China
    Copyright: © He et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 427
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    Published online on: March 30, 2021
       https://doi.org/10.3892/ol.2021.12688
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Abstract

Endometrial cancer (EC) is a multi‑factorial disease of which pathogenesis has not been fully elucidated. The function and underlying mechanism of microRNA‑20a‑5p (miR‑20a‑5p) in EC remain poorly understood. The present study aimed to analyze the association between miR‑20a‑5p expression and the clinicopathological characteristics of patients with EC. Whether miR‑20a‑5p could inhibit EC progression by targeting janus kinase 1 (Jak1) was subsequently investigated. To do so, human EC tissues and paracancerous tissues were collected from 47 patients with EC. miR‑20a‑5p and Jak1 mRNA and protein expression was determined by reverse transcription quantitative PCR and western blotting, respectively. Cell proliferation, invasive ability and adhesion were investigated by MTT, Matrigel invasion and cell adhesion assays, respectively. Dual luciferase reporter assay was used to verify whether miR‑20a‑5p could directly target Jak1. The results demonstrated that miR‑20a‑5p was downregulated and that Jak1 was upregulated in EC tissues compared with paracancerous tissues. In addition, miR‑20a‑5p expression and Jak1 expression level were negatively correlated in EC tissues. miR‑20a‑5p expression was also significantly associated with the depth of myometrial invasion, FIGO stage, histologic grade and lymph node metastasis in patients with EC. Furthermore, Jak1 was identified as a new direct target of miR‑20a‑5p, and Jak1 overexpression was demonstrated to reverse the effects of miR‑20a‑5p‑mimic on EC cell proliferation, invasive ability and adhesion. Taken together, the results from this study revealed for the first time that miR‑20a‑5p expression was significantly associated with the clinicopathological characteristics of patients with EC. These findings suggested that miR‑20a‑5p may act as a tumor suppressor in EC, in part through decreasing Jak1 expression. miR‑20a‑5p and Jak1 may therefore serve as potential therapeutic targets in EC.
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1 

Braun MM, Overbeek-Wager EA and Grumbo RJ: Diagnosis and management of endometrial cancer. Am Fam Physician. 93:468–474. 2016.PubMed/NCBI

2 

McAlpine JN, Temkin SM and Mackay HJ: Endometrial cancer: Not your grandmother's cancer. Cancer. 122:2787–2798. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Tsikouras P, Bouchlariotou S, Vrachnis N, Dafopoulos A, Galazios G, Csorba R and von Tempelhoff GF: Endometrial cancer: Molecular and therapeutic aspects. Eur J Obstet Gynecol Reprod Biol. 169:1–9. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Siegel RL, Miller KD and Jemal A: Cancer statistics, 2018. CA Cancer J Clin. 68:7–30. 2018. View Article : Google Scholar : PubMed/NCBI

6 

Colombo N, Creutzberg C, Amant F, Bosse T, González-Martín A, Ledermann J, Marth C, Nout R, Querleu D, Mirza MR, et al: ESMO-ESGO-ESTRO consensus conference on endometrial cancer: Diagnosis, treatment and follow-up. Ann Oncol. 27:16–41. 2016. View Article : Google Scholar : PubMed/NCBI

7 

Lu TX and Rothenberg ME: MicroRNA. J Allergy Clin Immunol. 141:1202–1207. 2018. View Article : Google Scholar : PubMed/NCBI

8 

Mohr AM and Mott JL: Overview of microRNA biology. Semin Liver Dis. 35:3–11. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Hutt S, Tailor A, Ellis P, Michael A, Butler-Manuel S and Chatterjee J: The role of biomarkers in endometrial cancer and hyperplasia: A literature review. Acta Oncol. 58:342–352. 2019. View Article : Google Scholar : PubMed/NCBI

10 

Stope MB, Koensgen D, Weimer J, Paditz M, Burchardt M, Bauerschlag D and Mustea A: The future therapy of endometrial cancer: microRNA's functionality, capability, and putative clinical application. Arch Gynecol Obstet. 294:889–895. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Rižner TL: Discovery of biomarkers for endometrial cancer: Current status and prospects. Expert Rev Mol Diagn. 16:1315–1336. 2016. View Article : Google Scholar : PubMed/NCBI

12 

Li HL, Sun JJ, Ma H, Liu SJ, Li N, Guo SJ, Shi Y, Xu YY, Qi ZY, Wang YQ, et al: MicroRNA-23a inhibits endometrial cancer cell development by targeting SIX1. Oncol Lett. 18:3792–3802. 2019.PubMed/NCBI

13 

Kong J, He X, Wang Y and Li J: Effect of microRNA-29b on proliferation, migration, and invasion of endometrial cancer cells. J Int Med Res. 47:3803–3817. 2019. View Article : Google Scholar : PubMed/NCBI

14 

Wang J, Zhang L, Jiang W, Zhang R, Zhang B, Silayiding A and Duan X: MicroRNA-135a promotes proliferation, migration, invasion and induces chemoresistance of endometrial cancer cells. Eur J Obstet Gynecol Reprod Biol X. 5:1001032020. View Article : Google Scholar : PubMed/NCBI

15 

Fuziwara CS and Kimura ET: Insights into Regulation of the miR-17-92 Cluster of miRNAs in Cancer. Front Med (Lausanne). 2:642015.PubMed/NCBI

16 

Agrawal S, Tapmeier T, Rahmioglu N, Kirtley S, Zondervan K and Becker C: The miRNA Mirage: How close are we to finding a non-invasive diagnostic biomarker in endometriosis? A systematic review. Int J Mol Sci. 19:5992018. View Article : Google Scholar

17 

Ramón LA, Braza-Boïls A, Gilabert J, Chirivella M, España F, Estellés A and Gilabert-Estellés J: MicroRNAs related to angiogenesis are dysregulated in endometrioid endometrial cancer. Hum Reprod. 27:3036–3045. 2012. View Article : Google Scholar : PubMed/NCBI

18 

Schwartz DM, Bonelli M, Gadina M and O'Shea JJ: Type I/II cytokines, JAKs, and new strategies for treating autoimmune diseases. Nat Rev Rheumatol. 12:25–36. 2016. View Article : Google Scholar : PubMed/NCBI

19 

Kleppe M, Kwak M, Koppikar P, Riester M, Keller M, Bastian L, Hricik T, Bhagwat N, McKenney AS, Papalexi E, et al: JAK-STAT pathway activation in malignant and nonmalignant cells contributes to MPN pathogenesis and therapeutic response. Cancer Discov. 5:316–331. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Mitchell TJ and John S: Signal transducer and activator of transcription (STAT) signalling and T-cell lymphomas. Immunology. 114:301–312. 2005. View Article : Google Scholar : PubMed/NCBI

21 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

22 

Wang L, Huang W, Ren C, Zhao M, Jiang X, Fang X and Xia X: Analysis of serum microRNA profile by solexa sequencing in women with endometriosis. Reprod Sci. 23:1359–1370. 2016. View Article : Google Scholar : PubMed/NCBI

23 

Jia SZ, Yang Y, Lang J, Sun P and Leng J: Plasma miR-17-5p, miR-20a and miR-22 are down-regulated in women with endometriosis. Hum Reprod. 28:322–330. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Lin SC, Wang CC, Wu MH, Yang SH, Li YH and Tsai SJ: Hypoxia-induced microRNA-20a expression increases ERK phosphorylation and angiogenic gene expression in endometriotic stromal cells. J Clin Endocrinol Metab. 97:E1515–E1523. 2012. View Article : Google Scholar : PubMed/NCBI

25 

Eismann J, Hirschfeld M, Erbes T, Rücker G, Jäger M, Ritter A, Weiss D, Gitsch G and Mayer S: Hypoxia- and acidosis-driven aberrations of secreted microRNAs in endometrial cancer in vitro. Oncol Rep. 38:993–1004. 2017. View Article : Google Scholar : PubMed/NCBI

26 

Zhang Y, Zheng L, Ding Y, Li Q, Wang R, Liu T, Sun Q, Yang H, Peng S, Wang W and Chen L: miR-20a induces cell radioresistance by activating the PTEN/PI3K/Akt signaling pathway in hepatocellular carcinoma. Int J Radiat Oncol Biol Phys. 92:1132–1140. 2015. View Article : Google Scholar : PubMed/NCBI

27 

Huang D, Bian G, Pan Y, Han X, Sun Y, Wang Y, Shen G, Cheng M, Fang X and Hu S: miR-20a-5p promotes radio-resistance by targeting Rab27B in nasopharyngeal cancer cells. Cancer Cell Int. 17:322017. View Article : Google Scholar : PubMed/NCBI

28 

Zhao W, Geng D, Li S, Chen Z and Sun M: LncRNA HOTAIR influences cell growth, migration, invasion, and apoptosis via the miR-20a-5p/HMGA2 axis in breast cancer. Cancer Med. 7:842–855. 2018. View Article : Google Scholar : PubMed/NCBI

29 

Cheng D, Zhao S, Tang H, Zhang D, Sun H, Yu F, Jiang W, Yue B, Wang J, Zhang M, et al: MicroRNA-20a-5p promotes colorectal cancer invasion and metastasis by downregulating Smad4. Oncotarget. 7:45199–45213. 2016. View Article : Google Scholar : PubMed/NCBI

30 

Huang Y and Yang N: MicroRNA-20a-5p inhibits epithelial to mesenchymal transition and invasion of endometrial cancer cells by targeting STAT3. Int J Clin Exp Pathol. 11:5715–5724. 2018.PubMed/NCBI

31 

Chen LT and Jiang CY: MicroRNA expression profiles identify biomarker for differentiating the embolic stroke from thrombotic stroke. Biomed Res Int. 2018:45141782018. View Article : Google Scholar : PubMed/NCBI

32 

Roskoski R Jr: Janus kinase (JAK) inhibitors in the treatment of inflammatory and neoplastic diseases. Pharmacol Res. 111:784–803. 2016. View Article : Google Scholar : PubMed/NCBI

33 

Sexl V, Kovacic B, Piekorz R, Moriggl R, Stoiber D, Hoffmeyer A, Liebminger R, Kudlacek O, Weisz E, Rothammer K and Ihle JN: Jak1 deficiency leads to enhanced Abelson-induced B-cell tumor formation. Blood. 101:4937–4943. 2003. View Article : Google Scholar : PubMed/NCBI

34 

Yeh YT, Ou-Yang F, Chen IF, Yang SF, Su JH, Hou MF and Yuan SS: Altered p-JAK1 expression is associated with estrogen receptor status in breast infiltrating ductal carcinoma. Oncol Rep. 17:35–39. 2007.PubMed/NCBI

35 

van der Zee M, Sacchetti A, Cansoy M, Joosten R, Teeuwssen M, Heijmans-Antonissen C, Ewing-Graham PC, Burger CW, Blok LJ and Fodde R: IL6/JAK1/STAT3 signaling blockade in endometrial cancer affects the ALDHhi/CD126+ stem-like component and reduces tumor burden. Cancer Res. 75:3608–3622. 2015. View Article : Google Scholar : PubMed/NCBI

36 

Ren Y, Zhang Y, Liu RZ, Fenstermacher DA, Wright KL, Teer JK and Wu J: JAK1 truncating mutations in gynecologic cancer define new role of cancer-associated protein tyrosine kinase aberrations. Sci Rep. 3:30422013. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
He Y, Ma H, Wang J, Kang Y and Xue Q: miR‑20a‑5p inhibits endometrial cancer progression by targeting janus kinase 1. Oncol Lett 21: 427, 2021.
APA
He, Y., Ma, H., Wang, J., Kang, Y., & Xue, Q. (2021). miR‑20a‑5p inhibits endometrial cancer progression by targeting janus kinase 1. Oncology Letters, 21, 427. https://doi.org/10.3892/ol.2021.12688
MLA
He, Y., Ma, H., Wang, J., Kang, Y., Xue, Q."miR‑20a‑5p inhibits endometrial cancer progression by targeting janus kinase 1". Oncology Letters 21.5 (2021): 427.
Chicago
He, Y., Ma, H., Wang, J., Kang, Y., Xue, Q."miR‑20a‑5p inhibits endometrial cancer progression by targeting janus kinase 1". Oncology Letters 21, no. 5 (2021): 427. https://doi.org/10.3892/ol.2021.12688
Copy and paste a formatted citation
x
Spandidos Publications style
He Y, Ma H, Wang J, Kang Y and Xue Q: miR‑20a‑5p inhibits endometrial cancer progression by targeting janus kinase 1. Oncol Lett 21: 427, 2021.
APA
He, Y., Ma, H., Wang, J., Kang, Y., & Xue, Q. (2021). miR‑20a‑5p inhibits endometrial cancer progression by targeting janus kinase 1. Oncology Letters, 21, 427. https://doi.org/10.3892/ol.2021.12688
MLA
He, Y., Ma, H., Wang, J., Kang, Y., Xue, Q."miR‑20a‑5p inhibits endometrial cancer progression by targeting janus kinase 1". Oncology Letters 21.5 (2021): 427.
Chicago
He, Y., Ma, H., Wang, J., Kang, Y., Xue, Q."miR‑20a‑5p inhibits endometrial cancer progression by targeting janus kinase 1". Oncology Letters 21, no. 5 (2021): 427. https://doi.org/10.3892/ol.2021.12688
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