Activation of GPER by E2 promotes proliferation, invasion and migration of breast cancer cells by regulating the miR‑124/CD151 pathway
- Huaicheng Yang
- Congyu Wang
- Heqiang Liao
- Qi Wang
Affiliations: Department of General Surgery, The Frist Affiliated Hospital of Anhui University of Science and Technology, Huainan, Anhui 232007, P.R. China
- Published online on: March 31, 2021 https://doi.org/10.3892/ol.2021.12693
Copyright: © Yang
et al. This is an open access article distributed under the
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Breast cancer is one of the most common malignancies worldwide and is responsible for a high mortality rate. However, the underlying pathological mechanism of breast cancer remains unclear. MicroRNAs (miRNAs/miRs) play critical roles in the progression of breast cancer. Recent studies have reported that miR‑124/CD151 participates in the development of breast cancer. However, the exact molecular mechanism of miR‑124/CD151 action in 17β‑estradiol (E2)‑treated breast cancer cells remains unknown. Thus, the present study aimed to investigate miR‑124 and CD151 expression levels in MCF‑7 cells treated with E2 via reverse transcription‑quantitative PCR and western blot analyses. Bioinformatic analysis was performed to predict and identify whether CD151 is a potential target of miR‑124. The Cell Counting Kit‑8 and colony formation assays were performed to detect proliferation of MCF‑7 cells. In addition, the invasive and migratory abilities of MCF‑7 cells were assessed via the Transwell and wound healing assays, respectively. The results demonstrated that E2 downregulated miR‑124 expression, while upregulating G protein ‑coupled estrogen receptor (GPER) expression in MCF‑7 cells. Following treatment with the GPER antagonist, G15, miR‑124 expression was significantly enhanced and E2‑induced proliferation, invasion and migration of MCF‑7 cells were notably inhibited. In addition, CD151 was confirmed as a direct target of miR‑124. CD151 silencing remarkably suppressed the proliferation, invasion and migration of E2‑induced MCF‑7 cells. Taken together, these results suggest that upregulation of GPER expression induced by E2 promotes proliferation, invasion and migration of breast cancer cells by regulating the miR‑124/CD151 pathway. Thus, the results of the present study provide a potential novel method for the treatment and prognosis of breast cancer.