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Epigenetic silencing of microRNA‑199a‑5p promotes the proliferation of non‑small cell lung cancer cells by increasing AKAP1 expression

  • Authors:
    • Nengli Yang
    • Yafeng Liang
    • Tianqi Zhu
    • Yanxiao Long
    • Zhe Chen
    • Xuezheng Zhang
    • Liuming Jiang
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China, Department of Pediatric Intensive Care Unit, The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 434
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    Published online on: March 31, 2021
       https://doi.org/10.3892/ol.2021.12695
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Abstract

MicroRNA (miR)‑199a‑5p expression is downregulated in a variety of malignancies, including non‑small cell lung cancer (NSCLC), and its low expression is associated with a poor prognosis. However, to the best of our knowledge, the mechanism underlying miR‑199a‑5p downregulation in NSCLC and its target effectors remain to be elucidated. The present study revealed the downregulation of miR‑199a‑5p expression in NSCLC tissues and cell lines compared with in para‑carcinoma tissues and a lung epithelial cell line. Further experiments indicated that the methylation levels of the miR‑199a promoter were markedly higher in NSCLC tissues compared with in para‑carcinoma tissues. The DNA methyltransferase inhibitor 5‑Aza‑2'‑deoxycytidine markedly increased the expression levels of miR‑199a‑5p in NSCLC cells. Furthermore, it was identified that miR‑199a‑5p mimics transfection decreased the expression levels of A‑kinase anchoring protein 1 (AKAP1) at both the mRNA and protein levels by targeting the 3' untranslated region of AKAP1 mRNA. The in vitro experiments demonstrated that miR‑199a‑5p overexpression inhibited the proliferation and tumorigenicity of NSCLC cells, whereas overexpression of AKAP1 partially recovered the malignant phenotypes, suggesting that AKAP1 may be a downstream effector targeted by miR‑199a‑5p. Collectively, the present findings indicated that miR‑199a‑5p may be a novel regulator of AKAP1, and that miR‑199a‑5p may be a potential tumor suppressor in NSCLC.
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Copy and paste a formatted citation
Spandidos Publications style
Yang N, Liang Y, Zhu T, Long Y, Chen Z, Zhang X and Jiang L: Epigenetic silencing of microRNA‑199a‑5p promotes the proliferation of non‑small cell lung cancer cells by increasing AKAP1 expression. Oncol Lett 21: 434, 2021.
APA
Yang, N., Liang, Y., Zhu, T., Long, Y., Chen, Z., Zhang, X., & Jiang, L. (2021). Epigenetic silencing of microRNA‑199a‑5p promotes the proliferation of non‑small cell lung cancer cells by increasing AKAP1 expression. Oncology Letters, 21, 434. https://doi.org/10.3892/ol.2021.12695
MLA
Yang, N., Liang, Y., Zhu, T., Long, Y., Chen, Z., Zhang, X., Jiang, L."Epigenetic silencing of microRNA‑199a‑5p promotes the proliferation of non‑small cell lung cancer cells by increasing AKAP1 expression". Oncology Letters 21.6 (2021): 434.
Chicago
Yang, N., Liang, Y., Zhu, T., Long, Y., Chen, Z., Zhang, X., Jiang, L."Epigenetic silencing of microRNA‑199a‑5p promotes the proliferation of non‑small cell lung cancer cells by increasing AKAP1 expression". Oncology Letters 21, no. 6 (2021): 434. https://doi.org/10.3892/ol.2021.12695
Copy and paste a formatted citation
x
Spandidos Publications style
Yang N, Liang Y, Zhu T, Long Y, Chen Z, Zhang X and Jiang L: Epigenetic silencing of microRNA‑199a‑5p promotes the proliferation of non‑small cell lung cancer cells by increasing AKAP1 expression. Oncol Lett 21: 434, 2021.
APA
Yang, N., Liang, Y., Zhu, T., Long, Y., Chen, Z., Zhang, X., & Jiang, L. (2021). Epigenetic silencing of microRNA‑199a‑5p promotes the proliferation of non‑small cell lung cancer cells by increasing AKAP1 expression. Oncology Letters, 21, 434. https://doi.org/10.3892/ol.2021.12695
MLA
Yang, N., Liang, Y., Zhu, T., Long, Y., Chen, Z., Zhang, X., Jiang, L."Epigenetic silencing of microRNA‑199a‑5p promotes the proliferation of non‑small cell lung cancer cells by increasing AKAP1 expression". Oncology Letters 21.6 (2021): 434.
Chicago
Yang, N., Liang, Y., Zhu, T., Long, Y., Chen, Z., Zhang, X., Jiang, L."Epigenetic silencing of microRNA‑199a‑5p promotes the proliferation of non‑small cell lung cancer cells by increasing AKAP1 expression". Oncology Letters 21, no. 6 (2021): 434. https://doi.org/10.3892/ol.2021.12695
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