Daphnoretin induces reactive oxygen species‑mediated apoptosis in melanoma cells

Wang,Hui; Hu,Xue; Li,Minjing; Pan,Zhaohai; Li,Defang; Zheng,Qiusheng

doi:10.3892/ol.2021.12714

Daphnoretin induces reactive oxygen species‑mediated apoptosis in melanoma cells

Authors:
- Hui Wang
- Xue Hu
- Minjing Li
- Zhaohai Pan
- Defang Li
- Qiusheng Zheng
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Affiliations: Key Laboratory of Xinjiang Phytomedicine Resource and Utilization, Ministry of Education, School of Pharmacy, Shihezi University, Shihezi, Xinjiang 832003, P.R. China, Yantai Key Laboratory of Pharmacology of Traditional Chinese Medicine in Tumor Metabolism, School of Integrated Traditional Chinese and Western Medicine, Binzhou Medical University, Yantai, Shandong 264003, P.R. China
Published online on: April 8, 2021 https://doi.org/10.3892/ol.2021.12714
Article Number: 453
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Research suggests that daphnoretin exhibits a diverse array of antitumor mechanisms and pharmacological activities. However, there is no definitive explanation for the antitumor mechanisms of daphnoretin in malignant melanoma. In the present study, MTT and colony formation assays demonstrated that daphnoretin significantly inhibited the proliferation of melanoma A375 and B16 cells. Following treatment with daphnoretin, apoptotic bodies were observed in A375 and B16 cells via Hoechst 33258 staining. Furthermore, western blot analysis revealed that the apoptosis‑related proteins cleaved caspase‑3, cleaved caspase‑9, Bax, cytochrome c and apoptotic protease‑activating factor 1 were significantly upregulated, while the expression levels of caspase‑3, caspase‑9 and Bcl‑2 were downregulated in A375 and B16 cells. Flow cytometry and fluorescence microscopy revealed that daphnoretin induced higher levels of reactive oxygen species (ROS). Therefore, the results of the present study indicated that daphnoretin induced ROS‑mediated mitochondria apoptosis in human (A375) and murine (B16) malignant melanoma cells.

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