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Daphnoretin induces reactive oxygen species‑mediated apoptosis in melanoma cells

  • Authors:
    • Hui Wang
    • Xue Hu
    • Minjing Li
    • Zhaohai Pan
    • Defang Li
    • Qiusheng Zheng
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Xinjiang Phytomedicine Resource and Utilization, Ministry of Education, School of Pharmacy, Shihezi University, Shihezi, Xinjiang 832003, P.R. China, Yantai Key Laboratory of Pharmacology of Traditional Chinese Medicine in Tumor Metabolism, School of Integrated Traditional Chinese and Western Medicine, Binzhou Medical University, Yantai, Shandong 264003, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 453
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    Published online on: April 8, 2021
       https://doi.org/10.3892/ol.2021.12714
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Abstract

Research suggests that daphnoretin exhibits a diverse array of antitumor mechanisms and pharmacological activities. However, there is no definitive explanation for the antitumor mechanisms of daphnoretin in malignant melanoma. In the present study, MTT and colony formation assays demonstrated that daphnoretin significantly inhibited the proliferation of melanoma A375 and B16 cells. Following treatment with daphnoretin, apoptotic bodies were observed in A375 and B16 cells via Hoechst 33258 staining. Furthermore, western blot analysis revealed that the apoptosis‑related proteins cleaved caspase‑3, cleaved caspase‑9, Bax, cytochrome c and apoptotic protease‑activating factor 1 were significantly upregulated, while the expression levels of caspase‑3, caspase‑9 and Bcl‑2 were downregulated in A375 and B16 cells. Flow cytometry and fluorescence microscopy revealed that daphnoretin induced higher levels of reactive oxygen species (ROS). Therefore, the results of the present study indicated that daphnoretin induced ROS‑mediated mitochondria apoptosis in human (A375) and murine (B16) malignant melanoma cells.
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Copy and paste a formatted citation
Spandidos Publications style
Wang H, Hu X, Li M, Pan Z, Li D and Zheng Q: Daphnoretin induces reactive oxygen species‑mediated apoptosis in melanoma cells. Oncol Lett 21: 453, 2021.
APA
Wang, H., Hu, X., Li, M., Pan, Z., Li, D., & Zheng, Q. (2021). Daphnoretin induces reactive oxygen species‑mediated apoptosis in melanoma cells. Oncology Letters, 21, 453. https://doi.org/10.3892/ol.2021.12714
MLA
Wang, H., Hu, X., Li, M., Pan, Z., Li, D., Zheng, Q."Daphnoretin induces reactive oxygen species‑mediated apoptosis in melanoma cells". Oncology Letters 21.6 (2021): 453.
Chicago
Wang, H., Hu, X., Li, M., Pan, Z., Li, D., Zheng, Q."Daphnoretin induces reactive oxygen species‑mediated apoptosis in melanoma cells". Oncology Letters 21, no. 6 (2021): 453. https://doi.org/10.3892/ol.2021.12714
Copy and paste a formatted citation
x
Spandidos Publications style
Wang H, Hu X, Li M, Pan Z, Li D and Zheng Q: Daphnoretin induces reactive oxygen species‑mediated apoptosis in melanoma cells. Oncol Lett 21: 453, 2021.
APA
Wang, H., Hu, X., Li, M., Pan, Z., Li, D., & Zheng, Q. (2021). Daphnoretin induces reactive oxygen species‑mediated apoptosis in melanoma cells. Oncology Letters, 21, 453. https://doi.org/10.3892/ol.2021.12714
MLA
Wang, H., Hu, X., Li, M., Pan, Z., Li, D., Zheng, Q."Daphnoretin induces reactive oxygen species‑mediated apoptosis in melanoma cells". Oncology Letters 21.6 (2021): 453.
Chicago
Wang, H., Hu, X., Li, M., Pan, Z., Li, D., Zheng, Q."Daphnoretin induces reactive oxygen species‑mediated apoptosis in melanoma cells". Oncology Letters 21, no. 6 (2021): 453. https://doi.org/10.3892/ol.2021.12714
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