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Article

MicroRNA‑133a‑3p suppresses malignant behavior of non‑small cell lung cancer cells by negatively regulating ERBB2

  • Authors:
    • Yanhui Xu
    • Lei Zhang
    • Lilong Xia
    • Xinhai Zhu
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic Surgery, Zhejiang Hospital, Hangzhou, Zhejiang 310030, P.R. China
  • Article Number: 457
    |
    Published online on: April 8, 2021
       https://doi.org/10.3892/ol.2021.12718
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Abstract

Non‑small cell lung cancer (NSCLC) has high morbidity and mortality rates worldwide, and tumor metastasis is generally associated with poor prognosis. Chemotherapy resistance aggravates the challenges associated with treating NSCLC. Therefore, identifying effective targets and developing therapies based on these findings could bring novel perspectives for patients with metastatic NSCLC. The expression levels of receptor tyrosine‑protein kinase erbB‑2 (ERBB2) are associated with NSCLC progression. Differential microRNA (miR) expression profiles have been identified in tumors and can be used to identify multiple malignant phenotypes. miR‑133a‑3p expression is dysregulated in a variety of tumors. However, to the best of our knowledge, the association between miR‑133a‑3p and the NSCLC pathogenesis process has not been demonstrated yet. The present study revealed a decrease in miR‑133a‑3p expression in both tissues and cell lines, which was detected using reverse transcription‑quantitative (RT‑q)PCR, and western blotting and RT‑qPCR demonstrated ERBB2 levels were increased at both protein and mRNA levels. Bioinformatics analysis and dual‑luciferase reporter assays demonstrated that ERBB2 was a direct target of miR‑133a‑3p. Furthermore, MTT, wound healing and Transwell assays revealed that overexpression of miR‑133a‑3p suppressed proliferation, invasion and migration of NSCLC cells, respectively, effects that were inhibited following ERBB2 overexpression. In addition, immunofluorescence assays demonstrated that overexpression of ERBB2 upregulated N‑cadherin expression, while E‑cadherin expression was downregulated. In conclusion, the present data demonstrated that miR‑133a‑3p acted as a tumor suppressor by negatively regulating ERBB2 expression. The miR‑133a‑3p/ERBB2 axis may be a potential target for the diagnosis and treatment of NSCLC in the future.
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Copy and paste a formatted citation
Spandidos Publications style
Xu Y, Zhang L, Xia L and Zhu X: MicroRNA‑133a‑3p suppresses malignant behavior of non‑small cell lung cancer cells by negatively regulating ERBB2. Oncol Lett 21: 457, 2021.
APA
Xu, Y., Zhang, L., Xia, L., & Zhu, X. (2021). MicroRNA‑133a‑3p suppresses malignant behavior of non‑small cell lung cancer cells by negatively regulating ERBB2. Oncology Letters, 21, 457. https://doi.org/10.3892/ol.2021.12718
MLA
Xu, Y., Zhang, L., Xia, L., Zhu, X."MicroRNA‑133a‑3p suppresses malignant behavior of non‑small cell lung cancer cells by negatively regulating ERBB2". Oncology Letters 21.6 (2021): 457.
Chicago
Xu, Y., Zhang, L., Xia, L., Zhu, X."MicroRNA‑133a‑3p suppresses malignant behavior of non‑small cell lung cancer cells by negatively regulating ERBB2". Oncology Letters 21, no. 6 (2021): 457. https://doi.org/10.3892/ol.2021.12718
Copy and paste a formatted citation
x
Spandidos Publications style
Xu Y, Zhang L, Xia L and Zhu X: MicroRNA‑133a‑3p suppresses malignant behavior of non‑small cell lung cancer cells by negatively regulating ERBB2. Oncol Lett 21: 457, 2021.
APA
Xu, Y., Zhang, L., Xia, L., & Zhu, X. (2021). MicroRNA‑133a‑3p suppresses malignant behavior of non‑small cell lung cancer cells by negatively regulating ERBB2. Oncology Letters, 21, 457. https://doi.org/10.3892/ol.2021.12718
MLA
Xu, Y., Zhang, L., Xia, L., Zhu, X."MicroRNA‑133a‑3p suppresses malignant behavior of non‑small cell lung cancer cells by negatively regulating ERBB2". Oncology Letters 21.6 (2021): 457.
Chicago
Xu, Y., Zhang, L., Xia, L., Zhu, X."MicroRNA‑133a‑3p suppresses malignant behavior of non‑small cell lung cancer cells by negatively regulating ERBB2". Oncology Letters 21, no. 6 (2021): 457. https://doi.org/10.3892/ol.2021.12718
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