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Article Open Access

Inhibition of aquaporins as a potential adjunct to breast cancer cryotherapy

  • Authors:
    • Haifa Alkhalifa
    • Fatima Mohammed
    • Sebastien Taurin
    • Khaled Greish
    • Safa Taha
    • Salim Fredericks
  • View Affiliations / Copyright

    Affiliations: Department of Basic Medical Sciences, Royal College of Surgeons in Ireland, Medical University of Bahrain, Adliya 15503, Kingdom of Bahrain, Department of Molecular Medicine, College of Medicine and Medical Sciences, Princess Al‑Jawhara Centre for Molecular Medicine, Arabian Gulf University, Segaya, Manama 328, Kingdom of Bahrain
    Copyright: © Alkhalifa et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 458
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    Published online on: April 8, 2021
       https://doi.org/10.3892/ol.2021.12719
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Abstract

Cryoablation is an emerging type of treatment for cancer. The sensitization of tumors using cryosensitizing agents prior to treatment enhances ablation efficiency and may improve clinical outcomes. Water efflux, which is regulated by aquaporin channels, contributes to cancer cell damage achieved through cryoablation. An increase in aquaporin (AQP) 3 is cryoprotective, whereas its inhibition augments cryodamage. The present study aimed to investigate aquaporin (AQP1, AQP3 and AQP5) gene expression and cellular localization in response to cryoinjury. Cultured breast cancer cells (MDA‑MB‑231 and MCF‑7) were exposed to freezing to induce cryoinjury. RNA and protein extracts were then analyzed using reverse transcription‑quantitative PCR and western blotting, respectively. Localization of aquaporins was studied using immunocytochemistry. Additionally, cells were transfected with small interfering RNA to silence aquaporin gene expression and cell viability was assessed using the Sulforhodamine B assay. Cryoinjury did not influence gene expression of AQPs, except for a 4‑fold increase of AQP1 expression in MDA‑MD‑231 cells. There were no clear differences in AQP protein expression for either cell lines upon exposure to frozen and non‑frozen temperatures, with the exception of fainter AQP5 bands for non‑frozen MCF‑7 cells. The exposure of cancer cells to freezing temperatures altered the localization of AQP1 and AQP3 proteins in both MCF‑7 and MDA‑MD‑231 cells. The silencing of AQP1, AQP3 and AQP5 exacerbated MDA‑MD‑231 cell damage associated with freezing compared with control siRNA. This was also observed with AQP3 and AQP5 silencing in MCF‑7 cells. Inhibition of aquaporins may potentially enhance cryoinjury. This cryosensitizing process may be used as an adjunct to breast cancer cryotherapy, especially in the border area targeted by cryoablation where freezing temperatures are not cold enough to induce cellular damage.
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Copy and paste a formatted citation
Spandidos Publications style
Alkhalifa H, Mohammed F, Taurin S, Greish K, Taha S and Fredericks S: Inhibition of aquaporins as a potential adjunct to breast cancer cryotherapy. Oncol Lett 21: 458, 2021.
APA
Alkhalifa, H., Mohammed, F., Taurin, S., Greish, K., Taha, S., & Fredericks, S. (2021). Inhibition of aquaporins as a potential adjunct to breast cancer cryotherapy. Oncology Letters, 21, 458. https://doi.org/10.3892/ol.2021.12719
MLA
Alkhalifa, H., Mohammed, F., Taurin, S., Greish, K., Taha, S., Fredericks, S."Inhibition of aquaporins as a potential adjunct to breast cancer cryotherapy". Oncology Letters 21.6 (2021): 458.
Chicago
Alkhalifa, H., Mohammed, F., Taurin, S., Greish, K., Taha, S., Fredericks, S."Inhibition of aquaporins as a potential adjunct to breast cancer cryotherapy". Oncology Letters 21, no. 6 (2021): 458. https://doi.org/10.3892/ol.2021.12719
Copy and paste a formatted citation
x
Spandidos Publications style
Alkhalifa H, Mohammed F, Taurin S, Greish K, Taha S and Fredericks S: Inhibition of aquaporins as a potential adjunct to breast cancer cryotherapy. Oncol Lett 21: 458, 2021.
APA
Alkhalifa, H., Mohammed, F., Taurin, S., Greish, K., Taha, S., & Fredericks, S. (2021). Inhibition of aquaporins as a potential adjunct to breast cancer cryotherapy. Oncology Letters, 21, 458. https://doi.org/10.3892/ol.2021.12719
MLA
Alkhalifa, H., Mohammed, F., Taurin, S., Greish, K., Taha, S., Fredericks, S."Inhibition of aquaporins as a potential adjunct to breast cancer cryotherapy". Oncology Letters 21.6 (2021): 458.
Chicago
Alkhalifa, H., Mohammed, F., Taurin, S., Greish, K., Taha, S., Fredericks, S."Inhibition of aquaporins as a potential adjunct to breast cancer cryotherapy". Oncology Letters 21, no. 6 (2021): 458. https://doi.org/10.3892/ol.2021.12719
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