Effect of the circCDR1as/miR‑641/XIAP regulatory axis on the proliferation and invasion of the prostate cancer PC‑3 cell line

Niu,Yulin; He,Jin Hua; Zhang,Yinglian; Li,Kun; Xing,Chungen

doi:10.3892/ol.2021.12730

Effect of the circCDR1as/miR‑641/XIAP regulatory axis on the proliferation and invasion of the prostate cancer PC‑3 cell line

Authors:
- Yulin Niu
- Jin Hua He
- Yinglian Zhang
- Kun Li
- Chungen Xing
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Affiliations: Department of Transplantation Surgery, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550004, P.R. China, Department of Laboratory Medicine, Central Hospital of Panyu District, Guangzhou, Guangdong 511400, P.R. China, Outpatient Department, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550004, P.R. China, Department of General Surgery, The Second Affiliated Hospital of Soochow University, Soochow, Jiangsu 215004, P.R. China
Published online on: April 13, 2021 https://doi.org/10.3892/ol.2021.12730
Article Number: 469
Copyright: © Niu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Prostate cancer is one of the most common malignant tumors in men. Patients with local infiltration and distant metastasis often have a poor prognosis. The present study aimed to investigate the expression and regulatory mechanism of the circular RNA cerebellar degeneration‑related protein 1, anti‑sense (circCDR1as) in prostate cancer cell lines. MicroRNAs (miRNAs) regulated by circCDR1as and target genes regulated by miRNAs were predicted using bioinformatics software. Prostate cancer cell lines (LNCaP, 22Rv1 and PC‑3), a normal prostate epithelial cell line (RWPE‑1) and a human embryonic kidney cell line (293T) were cultured. Relative gene expression was detected using reverse transcription PCR. Small interfering RNAs (siRNAs) targeting circCDR1as and X‑linked inhibitor of apoptosis protein (XIAP) and miRNA mimics were designed and transfected into the cell lines using Lipofectamine^® 3000. Cell invasion was determined using a Transwell assay, the cell proliferation rate was detected using an MTT assay and cell migration was examined using a scratch assay. Relative protein expression was detected using western blotting. Double fluorescent reporter gene vectors and an anti‑Ago2 RNA‑binding protein immunoprecipitation assay were used to verify binding. Bioinformatics analyses indicated that there was a binding site between miR‑641 and circCDR1as and between miR‑641 and XIAP. The expression of circCDR1as and XIAP was higher and the expression of miR‑641 was lower in the prostate cancer cell lines compared with the normal prostate epithelial cell line. After effectively reducing the expression of circCDR1as and XIAP and increasing the expression of miR‑641 in PC‑3 cells, the proliferation, invasion and migration of PC‑3 cells were effectively inhibited. circCDR1as could bind to miR‑641, which targeted the 3'‑untranslated region of XIAP. Reducing the expression of circCDR1 promoted the expression of miR‑641 and inhibited the expression of XIAP. Overall, the circCDR1as/miR‑641/XIAP regulatory axis plays a role in the invasion and migration of the prostate cancer PC‑3 cell line.

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1	Minimally invasive group of urology male genitourinary cancer professional committee of China anti cancer association, . Consensus of Chinese prostate cancer surgical treatment experts. Zhejiang Med. 40:217–220. 2018.
2	Welch HG and Albertsen PC: Reconsidering prostate cancer mortality-the Future of PSA screening. N Engl J Med. 382:1557–1563. 2020. View Article : Google Scholar : PubMed/NCBI
3	He JH, Zhang JZ, Han ZP, Wang L, Lv YB and Li YG: Reciprocal regulation of PCGEM1 and miR-145 promote proliferation of LNCaP prostate cancer cells. J Exp Clin Canc Res. 33:722014. View Article : Google Scholar : PubMed/NCBI
4	Huang S, Li X, Zheng H, Si X, Li B, Wei G, Li C, Chen Y, Chen Y, Liao W, et al: Loss of super-enhancer-regulated circRNA Nfix induces cardiac regeneration after myocardial infarction in adult mice. Circulation. 139:2857–2876. 2019. View Article : Google Scholar : PubMed/NCBI
5	Yang R, Xing L, Zheng X, Sun Y, Wang X and Chen J: The circRNA circAGFG1 acts as a sponge of miR-195-5p to promote triple-negative breast cancer progression through regulating CCNE1 expression. Mol Cancer. 18:42019. View Article : Google Scholar : PubMed/NCBI
6	Zhang K, Shi H, Xi H, Wu X, Cui J, Gao Y, Liang W, Hu C, Liu Y, Li J, et al: Genome-wide lncRNA microarray profiling identifies novel circulating lncRNAs for detection of gastric cancer. Theranostics. 7:213–227. 2017. View Article : Google Scholar : PubMed/NCBI
7	Zhang M, Ma L, Liu Y, He Y, Li G, An X and Cao B: CircRNA-006258 sponge-adsorbs miR-574-5p to regulate cell growth and milk synthesis via EVI5L in goat mammary epithelial cells. Genes (Basel). 11:7182020. View Article : Google Scholar : PubMed/NCBI
8	Xu L, Zhang M, Zheng X, Yi P, Lan C and Xu M: The circular RNA ciRS-7 (Cdr1as) acts as a risk factor of hepatic microvascular invasion in hepatocellular carcinoma. J Cancer Res Clin Oncol. 143:17–27. 2017. View Article : Google Scholar : PubMed/NCBI
9	Vo JN, Cieslik M, Zhang Y, Shukla S, Xiao L, Zhang Y, Wu YM, Dhanasekaran SM, Engelke CG, Cao X, et al: The landscape of circular RNA in cancer. Cell. 176:869–881.e13. 2019. View Article : Google Scholar : PubMed/NCBI
10	Do DN, Dudemaine PL, Fomenky BE and Ibeagha-Awemu EM: Integration of miRNA weighted gene co-expression network and miRNA-mRNA co-expression analyses reveals potential regulatory functions of miRNAs in calf rumen development. Genomics. 111:849–859. 2019. View Article : Google Scholar : PubMed/NCBI
11	Wang C, Zhou B, Liu M, Liu Y and Gao R: miR-126-5p restoration promotes cell apoptosis in cervical cancer by targeting Bcl2l2. Oncol Res. 25:463–470. 2017. View Article : Google Scholar : PubMed/NCBI
12	Sheng Y, Hu R, Zhang Y and Luo W: MicroRNA-4317 predicts the prognosis of breast cancer and inhibits tumor cell proliferation, migration, and invasion. Clin Exp Med. 20:417–425. 2020. View Article : Google Scholar : PubMed/NCBI
13	Liu T, Ye P, Ye Y, Lu S and Han B: Circular RNA hsa_circRNA_002178 silencing retards breast cancer progression via microRNA-328-3p-mediated inhibition of COL1A1. J Cell Mol Med. 24:21892020. View Article : Google Scholar : PubMed/NCBI
14	Barrera-Vázquez OS, Cancio-Lonches C, Hernández-González O, Chávez-Munguia B, Villegas-Sepúlveda N and Gutiérrez-Escolano AL: The feline calicivirus leader of the capsid protein causes survivin and XIAP downregulation and apoptosis. Virology. 527:146–158. 2019. View Article : Google Scholar
15	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
16	He JH, Han ZP, Zhou JB, Chen WM, Lv YB, He ML and Li YG: MiR-145 affected the circular RNA expression in prostate cancer LNCaP cells. J Cell Biochem. 119:9168–9177. 2018. View Article : Google Scholar : PubMed/NCBI
17	He JH, Han ZP, Zou MX, He ML, Li YG and Zheng L: CDX2/mir-145-5p/SENP1 pathways affect LNCaP cells invasion and migration. Front Oncol. 9:4772019. View Article : Google Scholar : PubMed/NCBI
18	Jiang XM, Li ZL, Li JL, Xu Y, Leng KM, Cui YF and Sun DJ: A novel prognostic biomarker for cholangiocarcinoma: circRNA Cdr1as. Eur Rev Med Pharmacol Sci. 22:365–371. 2018.PubMed/NCBI
19	Hansen TB, Jensen TI, Clausen BH, Bramsen JB, Finsen B, Damgaard CK and Kjems J: Natural RNA circles function as efficient microRNA sponges. Nature. 495:384–388. 2013. View Article : Google Scholar : PubMed/NCBI
20	Kong Q, Shu N, Li J and Xu N: miR-641 functions as a tumor suppressor by targeting MDM2 in human lung cancer. Oncol Res. 26:735–741. 2018. View Article : Google Scholar : PubMed/NCBI
21	Devi GR: XIAP as target for therapeutic apoptosis in prostate cancer. Drug News Perspect. 17:127–134. 2004. View Article : Google Scholar : PubMed/NCBI
22	Liu J, Li M and Xia S: Expression and clinical significance of antiapoptosis gene XIAP in prostate cancer. Zhonghua Nan Ke Xue. 10:832–835. 2004.(In Chinese). PubMed/NCBI
23	Berezovskaya O, Schimmer AD, Glinskii AB, Pinilla C, Hoffman RM, Reed JC and Glinsky GV: Increased expression of apoptosis inhibitor protein XIAP contributes to anoikis resistance of circulating human prostate cancer metastasis precursor cells. Cancer Res. 65:2378–2386. 2005. View Article : Google Scholar : PubMed/NCBI
24	Yu L, Gong X, Sun L, Zhou Q, Lu B and Zhu L: The circular RNA Cdr1as act as an oncogene in hepatocellular carcinoma through targeting miR-7 expression. PLoS One. 11:e01583472016. View Article : Google Scholar : PubMed/NCBI
25	Xu B, Yang T, Wang Z, Zhang Y, Liu S and Shen M: CircRNA CDR1as/miR-7 signals promote tumor growth of osteosarcoma with a potential therapeutic and diagnostic value. Cancer Manag Res. 10:4871–4880. 2018. View Article : Google Scholar : PubMed/NCBI
26	Yang W, Yang X, Wang X, Gu J, Zhou D, Wang Y, Yin B, Guo J and Zhou M: Silencing CDR1as enhances the sensitivity of breast cancer cells to drug resistance by acting as a miR-7 sponge to down-regulate REGγ. J Cell Mol Med. 23:4921–4932. 2019. View Article : Google Scholar : PubMed/NCBI
27	Chen H, Mao M, Jiang J, Zhu D and Li P: Circular RNA CDR1as acts as a sponge of miR-135b-5p to suppress ovarian cancer progression. Onco Targets Ther. 12:3869–3879. 2019. View Article : Google Scholar : PubMed/NCBI