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Upregulation of glucosamine‑phosphate N‑acetyltransferase 1 is a promising diagnostic and predictive indicator for poor survival in patients with lung adenocarcinoma

  • Authors:
    • Pengyuan Zhu
    • Shaorui Gu
    • Haitao Huang
    • Chongjun Zhong
    • Zhenchuan Liu
    • Xin Zhang
    • Wenli Wang
    • Shiliang Xie
    • Kaiqin Wu
    • Tiancheng Lu
    • Yongxin Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic and Cardiovascular Surgery, The Second Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China, Department of Thoracic and Cardiovascular Surgery, Tongji Hospital, School of Medicine, Tongji University, Shanghai 200065, P.R. China
    Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 488
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    Published online on: April 22, 2021
       https://doi.org/10.3892/ol.2021.12750
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Abstract

Lung adenocarcinoma, a type of non‑small cell lung cancer, is the leading cause of cancer death worldwide. Great efforts have been made to identify the underlying mechanism of adenocarcinoma, especially in relation to oncogenes. The present study by integrating computational analysis with western blotting, aimed to understand the role of the upregulation of glucosamine‑phosphate N‑acetyltransferase 1 (GNPNAT1) in carcinogenesis. In the present study, publicly available gene expression profiles and clinical data were downloaded from The Cancer Genome Atlas to determine the role of GNPNAT1 in lung adenocarcinoma (LUAD). In addition, the association between LUAD susceptibility and GNPNAT1 upregulation were analyzed using Wilcoxon signed‑rank test and logistic regression analysis. In LUAD, GNPNAT1 upregulation was significantly associated with disease stage [odds ratio (OR)=2.92, stage III vs. stage I], vital status (dead vs. alive, OR=1.89), cancer status (tumor status vs. tumor‑free status, OR=1.85) and N classification (yes vs. no, OR=1.75). Cox regression analysis and the Kaplan‑Meier method were utilized to evaluate the association between GNPNAT1 expression and overall survival (OS) time in patients with LUAD. The results demonstrated that patients with increased GNPNAT1 expression levels exhibited a reduced survival rate compared with those with decreased expression levels (P=8.9x10‑5). In addition, Cox regression analysis revealed that GNPNAT1 upregulation was significantly associated with poor OS time [hazard ratio (HR): 1.07; 95% confidence interval (CI): 1.04‑1.10; P<0.001]. The gene set enrichment analysis revealed that ‘cell cycle’, ‘oocyte meiosis’, ‘pyrimidine mediated metabolism’, ‘ubiquitin mediated proteolysis’, ‘one carbon pool by folate’, ‘mismatch repair progesterone‑mediated oocyte maturation’ and ‘basal transcription factors purine metabolism’ were differentially enriched in the GNPNAT1 high‑expression samples compared with GNPNAT1 low‑expression samples. The aforementioned pathways are involved in the pathogenesis of LUAD. The findings of the present study suggested that GNPNAT1 upregulation may be considered as a promising diagnostic and prognostic biomarker in patients with LUAD. In addition, the aforementioned pathways may be pivotal pathways perturbed by the abnormal expression of GNPNAT1 in LUAD. The findings of the present study demonstrated the therapeutic value of the regulation of GNPNAT1 in lung adenocarcinoma.
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Copy and paste a formatted citation
Spandidos Publications style
Zhu P, Gu S, Huang H, Zhong C, Liu Z, Zhang X, Wang W, Xie S, Wu K, Lu T, Lu T, et al: Upregulation of glucosamine‑phosphate N‑acetyltransferase 1 is a promising diagnostic and predictive indicator for poor survival in patients with lung adenocarcinoma. Oncol Lett 21: 488, 2021.
APA
Zhu, P., Gu, S., Huang, H., Zhong, C., Liu, Z., Zhang, X. ... Zhou, Y. (2021). Upregulation of glucosamine‑phosphate N‑acetyltransferase 1 is a promising diagnostic and predictive indicator for poor survival in patients with lung adenocarcinoma. Oncology Letters, 21, 488. https://doi.org/10.3892/ol.2021.12750
MLA
Zhu, P., Gu, S., Huang, H., Zhong, C., Liu, Z., Zhang, X., Wang, W., Xie, S., Wu, K., Lu, T., Zhou, Y."Upregulation of glucosamine‑phosphate N‑acetyltransferase 1 is a promising diagnostic and predictive indicator for poor survival in patients with lung adenocarcinoma". Oncology Letters 21.6 (2021): 488.
Chicago
Zhu, P., Gu, S., Huang, H., Zhong, C., Liu, Z., Zhang, X., Wang, W., Xie, S., Wu, K., Lu, T., Zhou, Y."Upregulation of glucosamine‑phosphate N‑acetyltransferase 1 is a promising diagnostic and predictive indicator for poor survival in patients with lung adenocarcinoma". Oncology Letters 21, no. 6 (2021): 488. https://doi.org/10.3892/ol.2021.12750
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu P, Gu S, Huang H, Zhong C, Liu Z, Zhang X, Wang W, Xie S, Wu K, Lu T, Lu T, et al: Upregulation of glucosamine‑phosphate N‑acetyltransferase 1 is a promising diagnostic and predictive indicator for poor survival in patients with lung adenocarcinoma. Oncol Lett 21: 488, 2021.
APA
Zhu, P., Gu, S., Huang, H., Zhong, C., Liu, Z., Zhang, X. ... Zhou, Y. (2021). Upregulation of glucosamine‑phosphate N‑acetyltransferase 1 is a promising diagnostic and predictive indicator for poor survival in patients with lung adenocarcinoma. Oncology Letters, 21, 488. https://doi.org/10.3892/ol.2021.12750
MLA
Zhu, P., Gu, S., Huang, H., Zhong, C., Liu, Z., Zhang, X., Wang, W., Xie, S., Wu, K., Lu, T., Zhou, Y."Upregulation of glucosamine‑phosphate N‑acetyltransferase 1 is a promising diagnostic and predictive indicator for poor survival in patients with lung adenocarcinoma". Oncology Letters 21.6 (2021): 488.
Chicago
Zhu, P., Gu, S., Huang, H., Zhong, C., Liu, Z., Zhang, X., Wang, W., Xie, S., Wu, K., Lu, T., Zhou, Y."Upregulation of glucosamine‑phosphate N‑acetyltransferase 1 is a promising diagnostic and predictive indicator for poor survival in patients with lung adenocarcinoma". Oncology Letters 21, no. 6 (2021): 488. https://doi.org/10.3892/ol.2021.12750
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